Corrigendum to “Biosynthesis of the diterpenoid steviol, an ent-kaurene derivative from Stevia rebaudiana Bertoni, via the methylerythritol phosphate pathway”

Totté, Nicole; Charon, Lionel; Rohmer, Michel; Compernolle, Frans; Baboeuf, Ilse; Geuns, Jan

doi:10.1016/s0040-4039(00)01297-1

Cited by 2 publications

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“…Both are expected to be synthesized mainly via the MEP way. For steviol glycosides, Hanson and White showed an incorporation of 0.83% into (−)-kaurene after feeding the cut stems of the plant with d , l -[2- 14 C]sodium mevalonate, which demonstrates a limited participation of the MVA pathway, but Totté et al , demonstrated that the MEP pathway was exclusively used for steviol glycoside biosynthesis by using labeling experiments with [1- 13 C]-glucose.…”

Section: Introductionmentioning

confidence: 99%

Methylerythritol and Mevalonate Pathway Contributions to Biosynthesis of Mono-, Sesqui-, and Diterpenes in Glandular Trichomes and Leaves of Stevia rebaudiana Bertoni

Wölwer‐Rieck

May

Lankes

et al. 2014

J. Agric. Food Chem.

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The biosynthesis of the diterpenoid steviol glycosides rebaudioside A and stevioside in nonrooted cuttings of Stevia rebaudiana was investigated by feeding experiments using the labeled key precursors [5,5-(2)H2]-mevalonic acid lactone (d2-MVL) and [5,5-(2)H2]-1-deoxy-d-xylulose (d2-DOX). Labeled glycosides were extracted from the leaves and stems and were directly analyzed by LC-(-ESI)-MS/MS and by GC-MS after hydrolysis and derivatization of the resulting isosteviol to the corresponding TMS-ester. Additionally, the incorporation of the proffered d2-MVL and d2-DOX into volatile monoterpenes, sesquiterpenes, and diterpenes in glandular trichomes on leaves and stems was investigated by headspace-solid phase microextraction-GC-MS (HS-SPME-GC-MS). Incorporation of the labeled precursors indicated that diterpenes in leaves and monoterpenes and diterpenes in glandular trichomes are predominately biosynthesized via the methylerythritol phosphate (MEP) pathway, whereas both the MEP and mevalonate (MVA) pathways contribute to the biosynthesis of sesquiterpenes at equal rates in glandular trichomes. These findings give evidence for a transport of MEP pathway derived farnesyl diphosphate precursors from plastids to the cytosol. Contrarily, the transport of MVA pathway derived geranyl diphosphate and geranylgeranyl diphosphate precursors from the cytosol to the plastid is limited.

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Section: Introductionmentioning

confidence: 99%

Methylerythritol and Mevalonate Pathway Contributions to Biosynthesis of Mono-, Sesqui-, and Diterpenes in Glandular Trichomes and Leaves of Stevia rebaudiana Bertoni

Wölwer‐Rieck

May

Lankes

et al. 2014

J. Agric. Food Chem.

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“…Steviol glycosides (SVglys) are ent -kaurenoic diterpenoids produced and accumulated in Stevia rebaudiana leaves up to 25% of the dry matter 1 . Biosynthesis of SVglys starts with the formation of geranyl-geranyl diphosphate (GGDP) in the chloroplast from the methyl-erythritol-4-phosphate (MEP) pathway 2 – 4 . GGDP then undergoes two cyclization steps, catalyzed by terpene cyclases to give ent -kaurene 5 .…”

Section: Introductionmentioning

confidence: 99%

Development of screening methods for functional characterization of UGTs from Stevia rebaudiana

Petit

Camborde

Vallejo

et al. 2020

Sci Rep

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Glycosylation is a key modification that contributes to determine bioactivity and bioavailability of plant natural products, including that of terpenoids and steviol glycosides (SVglys). It is mediated by uridine-diphosphate glycosyltransferases (UGTs), that achieve their activity by transferring sugars on small molecules. Thus, the diversity of SVglys is due to the number, the position and the nature of glycosylations on the hydroxyl groups in C-13 and C-19 of steviol. Despite the intense sweetener property of SVglys and the numerous studies conducted, the SVglys biosynthetic pathway remains largely unknown. More than 60 SVglys and 68 putative UGTs have been identified in Stevia rebaudiana. This study aims to provide methods to characterize UGTs putatively involved in SVglys biosynthesis. After agroinfiltration-based transient gene expression in Nicotiana benthamiana, functionality of the recombinant UGT can be tested simply and directly in plants expressing it or from a crude extract. The combined use of binary vectors from pGWBs series to produce expression vectors containing the stevia's UGT, enables functionality testing with many substrates as well as other applications for further analysis, including subcellular localization.

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Corrigendum to “Biosynthesis of the diterpenoid steviol, an ent-kaurene derivative from Stevia rebaudiana Bertoni, via the methylerythritol phosphate pathway”

Cited by 2 publications

References 0 publications

Methylerythritol and Mevalonate Pathway Contributions to Biosynthesis of Mono-, Sesqui-, and Diterpenes in Glandular Trichomes and Leaves of Stevia rebaudiana Bertoni

Methylerythritol and Mevalonate Pathway Contributions to Biosynthesis of Mono-, Sesqui-, and Diterpenes in Glandular Trichomes and Leaves of Stevia rebaudiana Bertoni

Development of screening methods for functional characterization of UGTs from Stevia rebaudiana

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