Corrigendum to “Identification of genes involved in the switch between the smooth and rugose phenotypes of Vibrio cholerae”

Rashid, Mohammed H.; Rajanna, Chythanya; Ali, Afsar; Karaolis, David K.R.

doi:10.1016/s0378-1097(03)00926-1-i1

Cited by 37 publications

(64 citation statements)

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“…The cyclic dinucleotide c-di-GMP is now recognized as a widespread intracellular signaling molecule in prokaryotes (2,3,(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). We have explored the extracellular biological effects of c-di-GMP and its potential use as a therapeutic agent.…”

Section: Discussionmentioning

confidence: 99%

“…The level of intracellular c-di-GMP in some species is believed to be regulated by the opposing effects of diguanylate cyclases for c-di-GMP synthesis from GTP and c-di-GMP phosphodiesterase for breakdown to 5Ј-GMP and each controlling the level of c-di-GMP in the cell. c-di-GMP has been shown to influence bacterial cell survival, cell differentiation, colonization and biofilm formation, as well as bacteria-host interactions (2,3,(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). Although the role of intracellular c-di-GMP in bacteria is now recognized and is becoming an intense field of research, we have been studying the effect of this microbial molecule in the context of other biological systems and its potential clinical and therapeutic use for the prevention and inhibition of infection and disease (19 -22).…”

Section: Bacterial C-di-gmp Is An Immunostimulatory Moleculementioning

confidence: 99%

“…We have previously demonstrated that exogenous c-di-GMP significantly reduces in vitro cell-cell interactions and biofilm formation of Staphylococcus aureus, including human methicillin-resistant S. aureus strains and animal clinical isolates (6,19). We have also previously demonstrated in vivo clinical proof-of-concept efficacy that treatment with c-di-GMP (200 nmol) significantly attenuates S. aureus infection and reduces cell numbers (Ͼ10,000-fold; p Ͻ 0.001) in a mouse model of infection (21).…”

Section: Bacterial C-di-gmp Is An Immunostimulatory Moleculementioning

confidence: 99%

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Bacterial c-di-GMP Is an Immunostimulatory Molecule

Karaolis

Means

Yang

et al. 2007

The Journal of Immunology

203

239

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Cyclic diguanylate (c-di-GMP) is a bacterial intracellular signaling molecule. We have shown that treatment with exogenous c-di-GMP inhibits Staphylococcus aureus infection in a mouse model. We now report that c-di-GMP is an immodulator and immunostimulatory molecule. Intramammary treatment of mice with c-di-GMP 12 and 6 h before S. aureus challenge gave a protective effect and a 10,000-fold reduction in CFUs in tissues (p < 0.001). Intramuscular vaccination of mice with c-di-GMP coinjected with S. aureus clumping factor A (ClfA) Ag produced serum with significantly higher anti-ClfA IgG Ab titers (p < 0.001) compared with ClfA alone. Intraperitoneal injection of mice with c-di-GMP activated monocyte and granulocyte recruitment. Human immature dendritic cells (DCs) cultured in the presence of c-di-GMP showed increased expression of costimulatory molecules CD80/CD86 and maturation marker CD83, increased MHC class II and cytokines and chemokines such as IL-12, IFN-γ, IL-8, MCP-1, IFN-γ-inducible protein 10, and RANTES, and altered expression of chemokine receptors including CCR1, CCR7, and CXCR4. c-di-GMP-matured DCs demonstrated enhanced T cell stimulatory activity. c-di-GMP activated p38 MAPK in human DCs and ERK phosphorylation in human macrophages. c-di-GMP is stable in human serum. We propose that cyclic dinucleotides like c-di-GMP can be used clinically in humans and animals as an immunomodulator, immune enhancer, immunotherapeutic, immunoprophylactic, or vaccine adjuvant.

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Section: Discussionmentioning

confidence: 99%

Section: Bacterial C-di-gmp Is An Immunostimulatory Moleculementioning

confidence: 99%

Section: Bacterial C-di-gmp Is An Immunostimulatory Moleculementioning

confidence: 99%

See 1 more Smart Citation

Bacterial c-di-GMP Is an Immunostimulatory Molecule

Karaolis

Means

Yang

et al. 2007

The Journal of Immunology

203

239

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“…It is thought that the adeptness of Vibrio cells at changing back and forth from either phenotypic variant would favor the variant derivative that is more fit for survival under the prevailing environment conditions, such as those encountered in a human host or on an environmental surface. Regulation of rugosity seems to be multifactorial and highly complex (5,17,26,36,37). Although there are some similarities, great differences are observed among organisms capable of expressing rugosity (3,36,37).…”

Section: Discussionmentioning

confidence: 99%

“…The presence of elongated cells within a rugose colony is also suggestive that these cells have down-regulated the need to divide and form cell wall septa. Rashid et al (26) reported that elongated cells of V. cholerae O1, which were deficient in recycling of cell wall material due to the loss of N-acetylmuramoyl-L-alanine amidase activity, also lacked the ability to form rugose colonies. Could these cells than be the source of the cells which make up the "ooze" material that we occasionally see with G. hollisae rugose colonies?…”

Section: Discussionmentioning

confidence: 99%

Rugosity in Grimontia hollisae

Curtis

Kothary

Blodgett

et al. 2007

Appl Environ Microbiol

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Grimontia hollisae, formerly Vibrio hollisae, produces both smooth and rugose colonial variants. The rugose colony phenotype is characterized by wrinkled colonies producing copious amounts of exopolysaccharide. Cells from a rugose colony grown at 30°C form rugose colonies, while the same cells grown at 37°C form smooth colonies, which are characterized by a nonwrinkled, uncrannied appearance. Stress response studies revealed that after exposure to bleach for 30 min, rugose survivors outnumbered smooth survivors.

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Genetic determinants of swimming motility in the squid light‐organ symbiont Vibrio fischeri

et al. 2013

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Bacterial flagellar motility is a complex cellular behavior required for the colonization of the light-emitting organ of the Hawaiian bobtail squid, Euprymna scolopes, by the beneficial bioluminescent symbiont Vibrio fischeri. We characterized the basis of this behavior by performing (i) a forward genetic screen to identify mutants defective in soft-agar motility, as well as (ii) a transcriptional analysis to determine the genes that are expressed downstream of the flagellar master regulator FlrA. Mutants with severe defects in soft-agar motility were identified due to insertions in genes with putative roles in flagellar motility and in genes that were unexpected, including those predicted to encode hypothetical proteins and cell division–related proteins. Analysis of mutants for their ability to enter into a productive symbiosis indicated that flagellar motility mutants are deficient, while chemotaxis mutants are able to colonize a subset of juvenile squid to light-producing levels. Thirty-three genes required for normal motility in soft agar were also downregulated in the absence of FlrA, suggesting they belong to the flagellar regulon of V. fischeri. Mutagenesis of putative paralogs of the flagellar motility genes motA motB, and fliL revealed that motA1 motB1, and both fliL1 and fliL2, but not motA2 and motB2, likely contribute to soft-agar motility. Using these complementary approaches, we have characterized the genetic basis of flagellar motility in V. fischeri and furthered our understanding of the roles of flagellar motility and chemotaxis in colonization of the juvenile squid, including identifying 11 novel mutants unable to enter into a productive light-organ symbiosis.

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Corrigendum to “Identification of genes involved in the switch between the smooth and rugose phenotypes of Vibrio cholerae”

Cited by 37 publications

References 0 publications

Bacterial c-di-GMP Is an Immunostimulatory Molecule

Bacterial c-di-GMP Is an Immunostimulatory Molecule

Rugosity in Grimontia hollisae

Genetic determinants of swimming motility in the squid light‐organ symbiont Vibrio fischeri

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