Corrigendum to “Molecular and macro-scale analysis of enzyme-crosslinked silk hydrogels for rational biomaterial design” [Acta Biomater. 63 (2017) 76–84]

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Full‐thickness skin wounds, associated with deep burns or chronic wounds pose a major clinical problem. Herein, the development of in situ forming hydrogel using a natural silk fibroin (SF) biomaterial for treating burn wounds is reported. Blends of SF solutions isolated from Bombyx mori and Antheraea assama show inherent self‐assembly between silk proteins and lead to irreversible gelation at body temperature. Investigation of the gelation mechanism reveals crosslinking due to formation of β‐sheet structures as examined by X‐ray diffraction and Fourier transform infrared spectroscopy. The SF hydrogel supports proliferation of primary human dermal fibroblasts and migration of keratinocytes comparable to collagen gel (Col) as examined under in vitro conditions. The SF hydrogel also provides an instructive and supportive matrix to the full‐thickness third‐degree burn wounds in vivo. A 3‐week comparative study with Col indicates that SF hydrogel not only promotes wound healing but also shows transitions from inflammation to proliferation stage as observed through the expression of TNF‐α and CD163 genes. Further, deposition and remodeling of collagen type I and III fibers suggests an enhanced overall tissue regeneration. Comparable results with Col demonstrate the SF hydrogel as an effective and inexpensive formulation toward a potential therapeutic approach for burn wound treatment.

Section: Resultssupporting

confidence: 69%

In Situ Forming Injectable Silk Fibroin Hydrogel Promotes Skin Regeneration in Full Thickness Burn Wounds

Chouhan

Lohe

Samudrala

2018

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128

“…2% w/v SELP hydrogels of S2E8Y 6mer, 8mer, and 12mer constructions were fabricated using horseradish peroxidase (HRP) mediated tyrosine crosslinking. [ 12 ] Following completion of the crosslinking reaction, the hydrogels were incubated in water at room temperature (≈20 °C) to swell the gels, as well as to remove residual HRP. The gels were then incubated at 65 °C (above the T t for all three protein gels) for 60 min to induce the thermal transition and the size changes from the initial room temperature conditions.…”

Section: Resultsmentioning

confidence: 99%

Smart Material Hydrogel Transfer Devices Fabricated with Stimuli‐Responsive Silk‐Elastin‐Like Proteins

Parker

Cairns

et al. 2020

Self Cite

Three‐dimensional organoid tissue culture models are a promising approach for the study of biological processes including diseases. Advances in these tissue culture technologies improve in vitro analysis compared to standard 2D cellular approaches and are more representative of the physiological environment. However, a major challenge associated with organoid systems stems from the laborious processing involved in the analysis of large numbers of organoids. Here the design, characterization, and application of silk‐elastin‐like protein‐based smart carrier arrays for processing organoids is presented. Fabrication of hydrogel‐based carrier systems at room temperature result in organized arrays of organoids that maintain tissue culture plate orientation and could be processed simultaneously for histology. The system works by transfer of the organoids to the hydrogel arrays after which the material is subjected to 65 °C to induce hydrogel contraction to secure the organoids, resulting in multisample constructs and allowing for placement on a microscope slide. Histological processing and immunostaining of these arrayed cerebral organoids analyzed within the contracted silk‐elastin‐like proteins (SELP) show retention of native organoid features compared to controls without the hydrogel carrier system, thus avoiding any artifacts. These SELP carriers present a useful approach for improving efficiency of scaled organoid screening and processing.

“…We hypothesize that the scaffolds memory‐shape feature observed can be related to both scaffolds architecture (micro‐ and macro‐porosity) and molecular changes resulting from enzymatic polymerization of tyrosine residues, which promote an increase in crosslinking density and ultimately led to an increase of the scaffolds elasticity. McGill et al reinforced the benefits and the tuning possibility of this HRP‐mediated crosslinking mechanism by modulating the silk and hydrogen peroxide concentration. The authors concluded that silk hydrogels have tunable matrices and by changing the reaction conditions, is possible to adjust not only the mechanical properties but also the diffusion behavior.…”

mentioning

confidence: 99%

Fast Setting Silk Fibroin Bioink for Bioprinting of Patient‐Specific Memory‐Shape Implants

Costa

Silva‐Correia

Oliveira

et al. 2017

The pursuit for the "perfect" biomimetic and personalized implant for musculoskeletal tissue regeneration remains a big challenge. 3D printing technology that makes use of a novel and promising biomaterials can be part of the solution. In this study, a fast setting enzymatic-crosslinked silk fibroin (SF) bioink for 3D bioprinting is developed. Their properties are fine-tuned and different structures with good resolution, reproducibility, and reliability can be fabricated. Many potential applications exist for the SF bioinks including 3D bioprinted scaffolds and patient-specific implants exhibiting unique characteristics such as good mechanical properties, memory-shape feature, suitable degradation, and tunable pore architecture and morphology.