Corrigendum to “Relation between growth dynamics and diffusional limitations in Saccharomyces cerevisiae cells growing as entrapped in an insolubilised gelatin gel” [FEMS Microbiol. Lett. 195 (2001) 245–251]

A strategic study using mutant‐strain entrapment in calcium alginate for the production of Saccharomyces cerevisiae cells with high invertase activity

“…Given the similarity of the diffusion coefficient of sucrose in water (5.68 × 10 −1 m 2 · s −1 ) and in calcium alginate gel (4.22 × 10 −1 m 2 s −1 ), Polakovic et al [21], Hannoun and Stephanopoulos [7] and Tanaka et al [6] suggest that in rich medium the entrapped cells in calcium alginate are not submitted to nutritional limitation, in agreement with de Alteriis et al [22]. The decrease of sugar-consumption rate in these entrapped cells may be due to non-adaptation of these cells to micro-environmental stress conditions inside of the matrix, but this problem may be resolved by previous cellular growth inside of the matrix.…”

“…The existence of chemical environmental gradients in entrapped-cell systems has been verified experimentally with various microprobe techniques [22,23]. The formation of a thick biofilm adjacent to the surface of the matrix can generate a gradient of nutrients and oxygen.…”

“…The growth of entrapped cells occurs under environmental conditions that may determine a different physiological response from the freely suspended cells. Further, during the incubation, massive colonization of the matrix leads to the formation of the particular microenvironment characterized by extreme cell proximity [22]. The S. cerevisiae invertase seems to be favoured by cellular growth in the entrapment gel.…”

“…Comparison of the SMC-1B and SMC-1B/3 strains shows that the cellular growth within the matrix reduces the catabolite-repression effect on invertase expression. Moreover, the combination of cell entrapment and nutrient gradient produces a spatial organization of gel-entrapped growing cells, which leads to the creation of a unique micro-environmental condition [22]. On the contrary, freely suspended cell cultures represent a transient system in which a continuously changing environment affects metabolic dynamics, such as those concerning invertase.…”