Cortical Bone Regeneration With a Synthetic Cell-Binding Peptide: A Histologic and Histomorphometric Pilot Study

Scarano, Antônio; Iezzi, Giovanna; Petrone, Giovanna; Orsini, Giovanna; Degidi, Marco; Strocchi, R.; Piattelli, Adriano

doi:10.1097/01.id.0000095467.48241.68

Cited by 39 publications

(50 citation statements)

References 10 publications

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“…Furthermore, the 9.0 ± 7.3% of residual ABM/P-15 revealed by histomorphometry may have continued to form new bone in vivo and added to the percentage of new bone formation, thus making the difference between the mean scores of the treatments even smaller with additional time (Table 3). The successful bone formation results achieved in this study with ABM/P-15 are consistent with results obtained in other preclinical models [14][15][16], dental models [17][18][19][20][21][22][23][24][25][26], and the pilot orthopaedic model [9].…”

Section: Discussionsupporting

confidence: 89%

“…Thus, patient satisfaction in lumbar fusions would likely rise if bone graft harvesting could be avoided by using a synthetic agent, like ABM/P-15, that is as effective as autologous bone. P-15 has been shown to be effective in several preclinical models [14][15][16]. A study by Thorwarth et al [15] studied the effectiveness of P-15 in porcine skull defects by creating four different treatment groups: autogenous bone, a porous algae-derived hydroxyapatite (adHA), P-15 and adHA, and P-15 and adHA combined with 25% autogenous bone.…”

Section: Discussionmentioning

confidence: 99%

“…This led to the conclusion that adHA/P-15 is an appropriate bone graft substitute for the correction of larger bony defects. In another study, Scarano et al [14] tested the effectiveness of ABM/P-15 in 8-mm tibial defects in rabbits versus no added agent. Using histomorphometric analysis at 4 weeks post surgery, they found a statistical significance in favor of ABM/P-15 for its ability to generate new bone.…”

Section: Discussionmentioning

confidence: 99%

“…Overall, the properties of ABM/P-15 provide a novel approach to facilitating bone growth while avoiding the additional morbidities associated with the harvest of autograft bone or the potential side effects and high cost of osteoinductive agents. P-15 has been shown to be effective in several preclinical models [14][15][16]. A study by Thorwarth et al [15] studied the effectiveness of P-15 in porcine skull defects while Scarano et al [14] studied ABM/P-15 in 8-mm tibial defects in rabbits.…”

Section: Introductionmentioning

confidence: 99%

“…P-15 has been shown to be effective in several preclinical models [14][15][16]. A study by Thorwarth et al [15] studied the effectiveness of P-15 in porcine skull defects while Scarano et al [14] studied ABM/P-15 in 8-mm tibial defects in rabbits. Both studies concluded that P-15 is an appropriate bone graft substitute to create new bone.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of ABM/P-15 versus autogenous bone in an ovine lumbar interbody fusion model

et al. 2010

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A prospective, randomized study was performed in an ovine model to compare the efficacy of an anorganic bovine-derived hydroxyapatite matrix combined with a synthetic 15 amino acid residue (ABM/P-15) in facilitating lumbar interbody fusion when compared with autogenous bone harvested from the iliac crest. P-15 is a biomimetic to the cell-binding site of Type-I collagen for bone-forming cells. When combined with ABM, it creates the necessary scaffold to initiate cell invasion, binding, and subsequent osteogenesis. In this study, six adult ewes underwent anterior-lateral interbody fusion at L3/L4 and L4/L5 using PEEK interbody rings filled with autogenous bone at one level and ABM/P-15 at the other level and no additional instrumentation. Clinical CT scans were obtained at 3 and 6 months; micro-CT scans and histomorphometry analyses were performed after euthanization at 6 months. Clinical CT scan analysis showed that all autograft and ABM/P-15 treated levels had radiographically fused outside of the rings at the 3-month study time point. Although the clinical CT scans of the autograft treatment group showed significantly better fusion within the PEEK rings than ABM/P-15 at 3 months, micro-CT scans, clinical CT scans, and histomorphometric analyses showed there were no statistical differences between the two treatment groups at 6 months. Thus, ABM/P-15 was as successful as autogenous bone graft in producing lumbar spinal fusion in an ovine model, and it should be further evaluated in clinical studies.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of ABM/P-15 versus autogenous bone in an ovine lumbar interbody fusion model

et al. 2010

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Regenerative Dentistry Using Stem Cells and Nanotechnology

Mitsiadis

Orsini

2016

Nanoscience and Nanotechnology for Human Health

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Efficacy of a small cell-binding peptide coated hydroxyapatite substitute on bone formation and implant fixation in sheep

Ding

Andreasen

Dencker

et al. 2014

J. Biomed. Mater. Res.

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Cylindrical critical size defects were created at the distal femoral condyles bilaterally of eight female adult sheep. Titanium implants with 2-mm concentric gaps were inserted and the gaps were filled with one of the four materials: allograft; a synthetic 15-amino acid cell-binding peptide coated hydroxyapatite (ABM/P-15); hydroxyapatite + βtricalciumphosphate+ Poly-Lactic-Acid (HA/βTCP-PDLLA); or ABM/P-15+HA/βTCP-PDLLA. After nine weeks, bone-implant blocks were harvested and sectioned for micro-CT scanning, push-out test, and histomorphometry. Significant bone formation and implant fixation could be observed in all four groups. Interestingly, the microarchitecture of the ABM/P-15 group was significantly different from the control group. Tissue volume fraction and thickness were significantly greater in the ABM/P-15 group than in the allograft group. Bone formation and bone ingrowth to porous titanium implant were not significantly different among the four groups. The ABM/P-15 group had similar shear mechanical properties on implant fixation as the allograft group. Adding HA/βTCP-PDLLA to ABM/P-15 did not significantly change these parameters. This study revealed that ABM/P-15 had significantly bone formation in concentric gap, and its enhancements on bone formation and implant fixation were at least as good as allograft. It is suggested that ABM/P-15 might be a good alternative biomaterial for bone implant fixation in this well-validated critical-size defect gap model in sheep. Nevertheless, future clinical researches should focus on prospective, randomized, controlled trials in order to fully elucidate whether ABM/P-15 could be a feasible candidate for bone substitute material in orthopedic practices.

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Cortical Bone Regeneration With a Synthetic Cell-Binding Peptide: A Histologic and Histomorphometric Pilot Study

Cited by 39 publications

References 10 publications

Evaluation of ABM/P-15 versus autogenous bone in an ovine lumbar interbody fusion model

Evaluation of ABM/P-15 versus autogenous bone in an ovine lumbar interbody fusion model

Regenerative Dentistry Using Stem Cells and Nanotechnology

Efficacy of a small cell-binding peptide coated hydroxyapatite substitute on bone formation and implant fixation in sheep

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