Cortical Microtubule Arrays Lose Uniform Alignment Between Cells and are Oryzalin Resistant in the Arabidopsis Mutant, radially swollen 6

Bannigan, Alex; Wiedemeier, Allison M. D.; Williamson, R. E.; Overall, Robyn L.; Baskin, Tobias I.

doi:10.1093/pcp/pcj067

Cited by 48 publications

(37 citation statements)

References 42 publications

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“…4B). Under higher magnification, this phenotype resembled the previously reported radially swollen mutants (19,20). No obvious shoot phenotype was detected, possibly based on the less severe RanGAP1 depletion in shoots.…”

Section: Inducible Rangap Depletion In Roots Leads To Incomplete and supporting

confidence: 88%

RanGAP1 is a continuous marker of the Arabidopsis cell division plane

Zhao

Rodrigo-Peiris

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

120

167

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In higher plants, the plane of cell division is faithfully predicted by the preprophase band (PPB). The PPB, a cortical ring of microtubules and F-actin, disassembles upon nuclear-envelope breakdown. During cytokinesis, the expanding cell plate fuses with the plasma membrane at the cortical division site, the site of the former PPB. The nature of the ''molecular memory'' that is left behind by the PPB and is proposed to guide the cell plate to the cortical division site is unknown. RanGAP is the GTPase activating protein of the small GTPase Ran, which provides spatial information for nucleocytoplasmic transport and various mitotic processes in animals. Here, we show that, in dividing root cells, Arabidopsis RanGAP1 concentrates at the PPB and remains associated with the cortical division site during mitosis and cytokinesis, requiring its N-terminal targeting domain. In a fass/ton2 mutant, which affects PPB formation, RanGAP1 recruitment to the PPB site is lost, while its PPB retention is microtubule-independent. RanGAP1 persistence at the cortical division site, but not its initial accumulation at the PPB requires the 2 cytokinesis-regulating kinesins POK1 and POK2. Depletion of RanGAP by inducible RNAi leads to oblique cell walls and cell-wall stubs in root cell files, consistent with cytokinesis defects. We propose that Arabidopsis RanGAP, a continuous positive protein marker of the plant division plane, has a role in spatial signaling during plant cell division.cytokinesis ͉ Ran cycle ͉ preprophase band ͉ nuclear pore

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Section: Inducible Rangap Depletion In Roots Leads To Incomplete and supporting

confidence: 88%

RanGAP1 is a continuous marker of the Arabidopsis cell division plane

Zhao

Rodrigo-Peiris

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

120

167

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“…Seedlings were grown for 5 d on 1% sucrose and then transferred onto plates containing 1% sucrose, 4.5% sucrose, or 1% sucrose plus 50 mM NaCl for 3 d. Seedlings were fixed, stained for microtubules, and imaged, all as described (Bannigan et al, 2006). Briefly, the fixative contained 4% paraformaldehyde, 1% glutaraldehyde, 50 mM PIPES, and 1 mM CaCl 2 .…”

Section: Analysis Of Microtubulesmentioning

confidence: 99%

Two Leucine-Rich Repeat Receptor Kinases Mediate Signaling, Linking Cell Wall Biosynthesis and ACC Synthase in Arabidopsis

et al. 2008

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The plant cell wall is a dynamic structure that changes in response to developmental and environmental cues through poorly understood signaling pathways. We identified two Leu-rich repeat receptor-like kinases in Arabidopsis thaliana that play a role in regulating cell wall function. Mutations in these FEI1 and FEI2 genes (named for the Chinese word for fat) disrupt anisotropic expansion and the synthesis of cell wall polymers and act additively with inhibitors or mutations disrupting cellulose biosynthesis. While FEI1 is an active protein kinase, a kinase-inactive version of FEI1 was able to fully complement the fei1 fei2 mutant. The expansion defect in fei1 fei2 roots was suppressed by inhibition of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, an enzyme that converts Ado-Met to ACC in ethylene biosynthesis, but not by disruption of the ethylene response pathway. Furthermore, the FEI proteins interact directly with ACC synthase. These results suggest that the FEI proteins define a novel signaling pathway that regulates cell wall function, likely via an ACC-mediated signal.

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“…Plant growth was as described (Bannigan et al, 2006). The rsw4 line was described previously (Wiedemeier et al, 2002).…”

Section: Plant Materials and Growthmentioning

confidence: 99%

“…The rsw4 line was described previously (Wiedemeier et al, 2002). The GFP-tubulin reporter line expresses GFP fused to the A. thaliana -tubulin 6 (TUB6) gene (Bannigan et al, 2006). The GFP-histone lines express GFP fused to the A. thaliana histone H3.3 gene (At5g10980) and were made by Keith Earley and Craig Pikaard (Washington University of St Louis).…”

Section: Plant Materials and Growthmentioning

confidence: 99%

“…For whole-mount observations, roots were fixed and treated as described for microtubule observation (Bannigan et al, 2006). For labeling DNA and microtubules, after the secondary antibody, seedlings were rinsed in PBS and treated with 1 g/ml RNase A for 1 hour at 37°C, followed by a PBS rinse and incubation in 3 M propidium iodide for 1 hour at room temperature.…”

Section: Microscopy and Histologymentioning

confidence: 99%

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A conditional mutation in Arabidopsis thaliana separase induces chromosome non-disjunction, aberrant morphogenesis and cyclin B1;1 stability

Scheible

Schindelasch

et al. 2010

Development

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SUMMARYThe caspase family protease, separase, is required at anaphase onset to cleave the cohesin complex, which joins sister chromatids. However, among eukaryotes, separases have acquired novel functions. Here, we show that Arabidopsis thaliana radially swollen 4 (rsw4), a temperature-sensitive mutant isolated previously on the basis of root swelling, harbors a mutation in At4g22970, the A. thaliana separase. Loss of separase function in rsw4 at the restrictive temperature is indicated by the widespread failure of replicated chromosomes to disjoin. Surprisingly, rsw4 has neither pronounced cell cycle arrest nor anomalous spindle formation, which occur in other eukaryotes upon loss of separase activity. However, rsw4 roots have disorganized cortical microtubules and accumulate the mitosis-specific cyclin, cyclin B1;1, excessive levels of which have been associated with altered microtubules and morphology. Cyclin B1;1 also accumulates in certain backgrounds in response to DNA damage, but we find no evidence for aberrant responses to DNA damage in rsw4. Our characterization of rsw4 leads us to hypothesize that plant separase, in addition to cleaving cohesin, regulates cyclin B1;1, with profound ramifications for morphogenesis.

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Cortical Microtubule Arrays Lose Uniform Alignment Between Cells and are Oryzalin Resistant in the Arabidopsis Mutant, radially swollen 6

Cited by 48 publications

References 42 publications

RanGAP1 is a continuous marker of the Arabidopsis cell division plane

RanGAP1 is a continuous marker of the Arabidopsis cell division plane

Two Leucine-Rich Repeat Receptor Kinases Mediate Signaling, Linking Cell Wall Biosynthesis and ACC Synthase in Arabidopsis

A conditional mutation in Arabidopsis thaliana separase induces chromosome non-disjunction, aberrant morphogenesis and cyclin B1;1 stability

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