Corticosteroidtherapie bei essentieller Eisenmangelanämie

Flenker, I; Neuhausen, P.; Ricken, D; Sabin, Gerald A.

doi:10.1007/978-3-642-85453-8_14

Cited by 1 publication

(1 citation statement)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Obviously macro creatine kinases are also protected from in vivo degradation. Free creatine kinase BB and macro creatine kinase in patients' sera are the two well documented causes for a false positive "CK-MB" test by the immunoinhibition procedure (4,5,6,7,8,23,24,25); therefore it may be of diagnostic interest to separate both of the enzyme forms. By the heating procedure macro creatine kinases decreased to a minimum of 63 percent of the initial activity, whereas the isoenzymes BB (MB) showed a residual activity of 9 (38) percent at the most.…”

Section: Discussionmentioning

confidence: 99%

Stability of Macro Creatine Kinases and Creatine Kinase Isoenzymes Compared: Heat Inactivation Test for Determination of Thermostable Creatine Kinases

Bohner¹,

Stein²,

Renn³

et al. 1981

CCLM

View full text Add to dashboard Cite

Summary: Creatine kinase isoenzymes showed decreasing thermal stability and increasing lability towards pH changes in the order: MM, MB, and BB. The three isoenzymes exhibited their highest stability between pH 6.5 and 7.0. At 37 °C and an almost physiological pH of 7.5 the decay constants were 0.025, 0.164, and 0.580 h" 1 (MM, MB, and BB isoenzyme), respectively.In contrast to free creatine kinase BB, immunoglobulin-linked creatine kinase BB (macro creatine kinase BB, type 1 macro creatine kinase) showed a markedly higher stability; this accounts for the persistence of creatine kinase BB activity in macro creatine kinasaemia. In addition we identified a second type of macro creatine kinase in patients' sera, which is also thermostable.A simple heat inactivation test (20 minutes, 45 °C, immunoinhibition of the M-subunits) differentiates thermostable macro creatine kinases from thermolabile creatine kinases and thus completes isoenzyme diagnosis.

show abstract