Corticosterone effects on differentiation and X-ray-induced transformation of C3H/10T1/2 mouse cells

Guernsey, Duane L.; Schmidt, Thomas J.

doi:10.1016/0045-6039(88)90067-x

Cited by 5 publications

(1 citation statement)

References 16 publications

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“…At least part of this discrepancy relates to the different culture conditions applied, corticosteroids probably being an important source of difference between early and recent culture systems. Corticosteroids initiate the differentiation of some fibroblastoid cells to adipogenic cells and subsequently to adipocytes (47,48). Preadipocytes may influence the haematopoietic process by virtue of their ability to secrete large amounts of fibronectin and laminin in the early stages of the adipogenesis.…”

Section: Effect Of In Vitro Irradiation On the Structural And Functiomentioning

confidence: 99%

The radiation response and recovery of bone marrow stroma with particular reference to long‐term bone marrow cultures

Bierkens

Hendry

Testa

1989

European J of Haematology

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There is evidence for long-term haematopoietic dysfunction in some patients treated with radiotherapy. Although the underlying mechanisms are unclear, both stem cell and environmental defects have been implicated. In the present article we review the evidence concerning the role of stromal cells. According to the endpoints used, a wide range of radiosensitivities for the stroma have been reported. Long-term bone marrow cultures provide a system in which both functional and regenerative aspects of the stroma can be studied. A dose of 5 Gy applied prior to the establishment of long-term bone marrow cultures decreases both the formation of a confluent adherent stromal layer and its capacity to support haematopoiesis. In contrast, in its fully established phase, the adherent layer displays a high radioresistance due to the low proliferative stress applied to its stromal populations. A dose of 10 Gy given to a fully established adherent layer does not prevent haematopoietic engraftment and sustained haematopoiesis. At doses above 100 Gy a macrophage-like and epithelioid cell-type become dominant, which preserve their ability of producing growth regulatory molecules at doses as high as 500 Gy. These data suggest that the main effect on the stroma is a delayed expression of irradiation damage due to the slow rate of turnover of stromal cells. So far, there is little evidence for persistent deficiencies in the functional roles of stromal cell populations.

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Section: Effect Of In Vitro Irradiation On the Structural And Functiomentioning

confidence: 99%

The radiation response and recovery of bone marrow stroma with particular reference to long‐term bone marrow cultures

Bierkens

Hendry

Testa

1989

European J of Haematology

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Dependence of mesenchymal cell responses on duration of exposure to bone morphogenetic protein-2 in vitro

Puleo

1997

J. Cell. Physiol.

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Bone morphogenetic proteins (BMPs) induce osteoblastic responses in cultures of pluripotent mesenchymal cells. The effects of chronic treatment of these cells with BMPs and of withdrawal following exposure, however, have not been fully elucidated. Thus, the aim of this study was to obtain information about the duration of exposure to recombinant human BMP-2 (rhBMP-2) required for expression and retention of osteoblastic characteristics with subsequent formation of a mineralized extracellular matrix in mesenchymal cell cultures. C3H1OT1/2 cells and bone marrow stromal cells were cultured with 1 mug/ml rhBMP-2 for either 0, 7, 14, 21, or 28 days, with the remainder of the 4 week total culture period in the absence of rhBMP-2. Growth and expression of osteoblastic characteristics were examined at the end of each week. C3H1OT1/2 cells responded to increasing duration of exposure to rhBMP-2 with increased cell growth. Additionally, the longer the cells were exposed to rhBMP-2, the more fully they expressed and sustained osteoblastic traits, i.e., they exhibited duration of exposure-dependent higher levels of alkaline phosphatase and osteocalcin and larger total amounts of mineral in the matrix. In comparison, exposure of bone marrow stromal cells to rhBMP-2 for at least 14 days restrained cell growth and prevented detachment. With respect to osteoblastic traits, stromal cells exposed to rhBMP-2 also exhibited a dependence on the duration of exposure, however, cultures treated for 14, 21, or 28 days exhibited similar levels of alkaline phosphatase activity and comparable amounts of calcium in the mineralizing matrix.

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Mesodermal cell lineage determination and proto-oncogene expression

Ketelaar

Utesch

Guernsey

et al. 1988

Cell Differentiation and Development

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Corticosterone effects on differentiation and X-ray-induced transformation of C3H/10T1/2 mouse cells

Cited by 5 publications

References 16 publications

The radiation response and recovery of bone marrow stroma with particular reference to long‐term bone marrow cultures

The radiation response and recovery of bone marrow stroma with particular reference to long‐term bone marrow cultures

Dependence of mesenchymal cell responses on duration of exposure to bone morphogenetic protein-2 in vitro

Mesodermal cell lineage determination and proto-oncogene expression

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