Corticotropin-Releasing Hormone-Binding Protein and its Possible Role in Neuroendocrinological Research

Zhao, Xin-Yue; Hoheisel, G; Schauer, Jenna; Bornstein, S. R.

doi:10.1055/s-2007-979058

Cited by 16 publications

(6 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rodent CRF-BP blocks CRF-induced adrenocorticotropic hormone (ACTH) release from pituitary cells in vitro (Potter et al 1991, Cortright et al 1995. Also, CRF released by the human placenta rises exponentially during pregnancy (McLean et al 1995), but most plasma CRF is bound to its binding protein and therefore unable to bind to its receptors (Zhao et al 1997). The binding of CRF to plasma CRF-BP would prevent inappropriate stimulation of the stress axis by placental CRF (Petraglia et al 1993).…”

Section: Introductionmentioning

confidence: 99%

Stress elevates corticotropin-releasing factor (CRF) and CRF-binding protein mRNA levels in rainbow trout (Oncorhynchus mykiss)

Doyon

Trudeau

Moon³

2005

Journal of Endocrinology

View full text Add to dashboard Cite

The objectives of this study were to characterize rainbow trout (Oncorhynchus mykiss) corticotropin-releasing factor (CRF)-binding protein (CRF-BP) cDNA and to examine the variations in CRF-BP and CRF mRNA levels in response to different intensities of stress. Trout were physically disturbed by a single or three consecutive periods of chasing until exhaustion followed by 2 h of recovery. The pituitary CRF-BP and preoptic area CRF1 mRNA contents were significantly increased only after repeated chasing events. Physical disturbance increased plasma cortisol levels with the largest change occurring in the group of trout that were exposed to repeated chasing events. Trout were also individually isolated in 120 l tanks or confined to 1·5 l boxes for 4, 24 or 72 h. CRF-BP mRNA levels in confined fish were greater than those of isolated fish at 72 h although there were no differences compared with the control group. CRF1 mRNA levels in the preoptic area were greater and remained elevated for a longer period in confined compared with isolated trout. Isolation led to a transient increase in plasma cortisol levels, but the higher cortisol values developed in the confined fish suggest that this treatment was more stressful than isolation. These results demonstrate that the intensity and duration of stress are important factors regulating CRF and CRF-BP mRNA levels in rainbow trout. We hypothesize that pituitary CRF-BP is involved in regulating the activity of the stress axis, possibly by reducing access to CRF1 receptors in the corticotropes.

show abstract

Section: Introductionmentioning

confidence: 99%

Stress elevates corticotropin-releasing factor (CRF) and CRF-binding protein mRNA levels in rainbow trout (Oncorhynchus mykiss)

Doyon

Trudeau

Moon³

2005

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…The corticotropin-releasing hormone (CRH), its binding protein (CRH-BP), and receptors (CRH-R, types 1 and 2) act as a central regulatory system of the HPA axis (4,7). Pro-CRH processing into CRH appears to be similar at the central and peripheral levels, including the skin (4).…”

mentioning

confidence: 99%

Corticotropin-releasing hormone: An autocrine hormone that promotes lipogenesis in human sebocytes

Zouboulis

Seltmann²,

Hiroi³

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

261

225

View full text Add to dashboard Cite

Sebaceous glands may be involved in a pathway conceptually similar to that of the hypothalamic-pituitary-adrenal (HPA) axis. Such a pathway has been described and may occur in human skin and lately in the sebaceous glands because they express neuropeptide receptors. Corticotropin-releasing hormone (CRH) is the most proximal element of the HPA axis, and it acts as central coordinator for neuroendocrine and behavioral responses to stress. To further examine the probability of an HPA equivalent pathway, we investigated the expression of CRH, CRH-binding protein (CRH-BP), and CRH receptors (CRH-R) in SZ95 sebocytes in vitro and their regulation by CRH and several other hormones. CRH, CRH-BP, CRH-R1, and CRH-R2 were detectable in SZ95 sebocytes at the mRNA and protein levels: CRH-R1 was the predominant type (CRH-R1͞CRH-R2 ‫؍‬ 2). CRH was biologically active on human sebocytes: it induced biphasic increase in synthesis of sebaceous lipids with a maximum stimulation at 10 ؊7 M and up-regulated mRNA levels of 3␤-hydroxysteroid dehydrogenase͞⌬ 5-4 isomerase, although it did not affect cell viability, cell proliferation, or IL-1␤-induced IL-8 release. CRH, dehydroepiandrosterone, and 17␤-estradiol did not modulate CRH-R expression, whereas testosterone at 10 ؊7 M down-regulated CRH-R1 and CRH-R2 mRNA expression at 6 to 24 h, and growth hormone (GH) switched CRH-R1 mRNA expression to CRH-R2 at 24 h. Based on these findings, CRH may be an autocrine hormone for human sebocytes that exerts homeostatic lipogenic activity, whereas testosterone and growth hormone induce CRH negative feedback. The findings implicate CRH in the clinical development of acne, seborrhea, androgenetic alopecia, skin aging, xerosis, and other skin disorders associated with alterations in lipid formation of sebaceous origin.

show abstract

“…In this respect, the significant differences between the CRH pro− moter haplotypes for dexamethasone−mediated suppression of LUC activity we observed may be attributed to the varying re− sponsiveness on cAMP stimulation as described above. Thus, the polymorphic promoter sequences we studied may play a role in fine−tuning the response of the CRH gene expression to various stimuli together with potential additional factors [43].…”

Section: Discussionmentioning

confidence: 99%

Promoter Polymorphisms Regulating Corticotrophin-releasing Hormone TranscriptionIn Vitro

Wagner

Wahle²,

Moritz³

et al. 2006

Horm Metab Res

View full text Add to dashboard Cite

To investigate whether polymorphisms in the corticotrophin-releasing hormone (CRH) promoter are associated with altered CRH gene regulation, we studied the reactivity of three recently described promoter variants in vitro. The 3625 bp variants A1B1, A2B1 and A2B2 of the human CRH promoter were cloned in the 5' region to a luciferase reporter gene and transiently transfected into both mouse anterior pituitary cells AtT-20D16vF2 and pheochromocytoma cells PC12. Incubation with 8-Br-cAMP alone or in combination with cytokines significantly enhanced the promoter activity in both cell lines studied by up to 22-fold. However, dexamethasone antagonised cAMP effects on CRH expression in AtT-20 cells while showing no effect on PC12 cells, indicating that tissue-specific factors play a crucial role. Among the haplotypes studied, A1B1 exhibited the greatest reactivity on various stimuli. Electric mobility shift assay (EMSA) was performed to study whether the described polymorphic nucleotide sequences in the 5' region of the hCRH gene interfere with binding of nuclear proteins. A specific DNA protein complex was detected at position -2353 bp for the wild type sequence only, possibly interfering with a binding site for the activating transcription factor 6 (ATF6). Taken together, this is the first study to demonstrate that CRH promoter reactivity varies between the compound promoter alleles.

show abstract

Corticotropin-Releasing Hormone-Binding Protein and its Possible Role in Neuroendocrinological Research

Cited by 16 publications

References 26 publications

Stress elevates corticotropin-releasing factor (CRF) and CRF-binding protein mRNA levels in rainbow trout (Oncorhynchus mykiss)

Stress elevates corticotropin-releasing factor (CRF) and CRF-binding protein mRNA levels in rainbow trout (Oncorhynchus mykiss)

Corticotropin-releasing hormone: An autocrine hormone that promotes lipogenesis in human sebocytes

Promoter Polymorphisms Regulating Corticotrophin-releasing Hormone TranscriptionIn Vitro

Contact Info

Product

Resources

About