Corticotropin-releasing hormone inhibits in vitro oocyte maturation in mice

Kiapekou, Erasmia; Zapanti, Evangelia; Voukelatou, Dionysia; Mavreli, Theodora; Stefanidis, Konstantinos; Drakakis, Peter; Mastorakos, George; Loutradis, Dimitris

doi:10.1016/j.fertnstert.2010.12.023

Cited by 13 publications

(10 citation statements)

References 24 publications

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“…In our previous study, we demonstrated CRH-associated inhibition in the resumption of meiosis in mouse oocytes through activation of CRH-Rs, which lead to adenylate cyclase activation and subsequent cAMP elevation (16). Herein, although we did not directly assess fertilization rate via two-pronuclei-stage tracing, we found a significant difference in the percentage of two-cell-stage embryos between CRH and control group, suggesting a negative effect of CRH on oocyte cytoplasmic maturation reflected by early embryo development.…”

Section: Discussionmentioning

confidence: 95%

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The Effect of CRH and Its Inhibitor, Antalarmin, on in Vitro Growth of Preantral Mouse Follicles, Early Embryo Development, and Steroidogenesis

et al. 2013

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In vitro growth systems of preantral follicles allow studying the effect of various endocrine, paracrine, and autocrine factors on follicular growth and oocyte maturation. CRH is a 41-amino-acid neuropeptide responsible for endocrine, autonomic, immunological, and behavioral responses of mammals to stress and has two receptors, CRH receptor type 1 (CRH-R1) and CRH-R2. Antalarmin, a CRH-R1 antagonist, has been used to elucidate the role of CRH in stress, inflammation, and reproduction. The present study describes in vitro growth of mouse preantral follicles, early embryo development, and steroidogenesis in the presence of CRH and its antagonist antalarmin. We cultured 732 follicles in control media, 1306 in CRH 10(-7) mol/liter, and 1202 in CRH 10(-7) plus antalarmin 10(-6) mol/liter. The culture medium was assayed on alternate days for 17β-estradiol, progesterone, and β-human chorionic gonadotropin. Total RNA was extracted from preantral follicles as well as early preimplantation embryos and was assessed by real-time RT-PCR for the expression of CRH-R1 and CRH-R2 mRNAs. Hormone analysis showed that the CRH group had lower levels of 17β-estradiol, progesterone, and β-human chorionic gonadotropin as the culture progressed, in comparison with the other two groups. RT-PCR demonstrated the presence of CRH-R1 and CRH-R2 in all stages of preantral follicle culture. Morula/blastocyst-stage embryos expressed only CRH-R1. In conclusion, CRH has an inhibitory effect on in vitro fertilized oocytes, resulting from cultured preantral follicles at all stages of preimplantation embryo development. Furthermore, the presence of CRH in the culture medium inhibits steroidogenesis by preantral mouse follicles cultured in vitro.

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Section: Discussionmentioning

confidence: 95%

“…Our previous study demonstrated that CRH inhibits in vitro oocyte maturation in mice at the concentration of 10 Ϫ7 mol/liter, and antalarmin reverses this effect at the concentration of 10 Ϫ6 mol/liter (16). In addition, the concentration of CRH 10 Ϫ7 mol/liter is capable of suppressing FSH-stimulated (1 IU/liter) estrogen release by about 30% (17).…”

mentioning

confidence: 95%

The Effect of CRH and Its Inhibitor, Antalarmin, on in Vitro Growth of Preantral Mouse Follicles, Early Embryo Development, and Steroidogenesis

et al. 2013

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“…The CRH1 receptor is also found in a number of peripheral tissues, for example, in the female reproductive tract (Nappi & Rivest 1995, Kiapekou et al 2011, skin (Slominiski et al 1995), adrenal gland (Willenberg et al 2006, Squillacioti et al 2011) and gastrointestinal (GI) tract (Chatzaki et al 2004). Among teleost fish, the Crh1 receptor is expressed in a number of tissues.…”

Section: Journal Of Molecular Endocrinologymentioning

confidence: 99%

“…CRH1 and CRH2 receptors cluster as discrete groups and represent together a sister lineage of the insect DH receptors. Species abbreviation, species name and accession name are indicated: ADOME: Acheta domesticus, Q16983.1; AAEGYI: Aedes aegypti, ABX57919.1; development in vitro likely through a CRH1-mediated receptor action (Kiapekou et al 2011). In monkeys, expression of CRH, UCN and the CRH1 receptor in luteal cells varies as a function of the monkey's menstrual cycle, suggesting that the CRH system and its requisite receptors act, in part, to regulate follicular growth and development (Xu et al 2007).…”

Section: :3 T52mentioning

confidence: 99%

MOLECULAR EVOLUTION OF GPCRS: CRH/CRH receptors

Lovejoy¹,

Chang²,

Lovejoy³

et al. 2014

Journal of Molecular Endocrinology

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Corticotrophin-releasing hormone (CRH) is the pivotal neuroendocrine peptide hormone associated with the regulation of the stress response in vertebrates. However, CRH-like peptides are also found in a number of invertebrate species. The origin of this peptide can be traced to a common ancestor of lineages leading to chordates and to arthropods, postulated to occur some 500 million years ago. Evidence indicates the presence of a single CRH-like receptor and a soluble binding protein system that acted to transduce and regulate the actions of the early CRH peptide. In vertebrates, genome duplications led to the divergence of CRH receptors into CRH1 and CRH2 forms in tandem with the development of four paralogous ligand lineages that included CRH; urotensin I/urocortin (Ucn), Ucn2 and Ucn3. In addition, taxon-specific genome duplications led to further local divergences in CRH ligands and receptors. Functionally, the CRH ligand-receptor system evolved initially as a molecular system to integrate early diuresis and nutrient acquisition. As multicellular organisms evolved into more complex forms, this ligand-receptor system became integrated with the organismal stress response to coordinate homoeostatic challenges with internal energy usage. In vertebrates, CRH and the CRH1 receptor became associated with the hypothalamo-pituitary-adrenal/interrenal axis and the initial stress response, whereas the CRH2 receptor was selected to play a greater role in diuresis, nutrient acquisition and the latter aspects of the stress response.

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“…Recently we examined the role of CRH and the CRH antagonist antalarmin in long term mouse follicle cultures 34 . We found by employing RT‐PCR, that CRH‐R1 mRNA is expressed in mouse preantral follicles, COCs, GV stage oocytes and PB oocytes.…”

Section: Role Of Ovarian Crh In Oocyte Growth and Maturationmentioning

confidence: 99%

Update on the role of ovarian corticotropin‐releasing hormone

Kiapekou

Zapanti

Mastorakos

et al. 2010

Annals of the New York Academy of Sciences

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Corticotropin-releasing hormone (CRH) is a 41-amino acid peptide synthesized by neurons of the parvocellular and paraventricular hypothalamic nuclei. Central CRH is the principal regulator of the stress system influencing several systems in the brain and influenced by them. It activates the secretion of glucocorticoids and indirectly regulates the immune system and the immune response. Peripheral CRH is secreted by postganglionic sympathetic and unmyelinated sensory afferent neurons and has been identified in several peripheral tissues and organs, including those of the reproductive system (ovary, endometrium, placenta, and testis). In the human ovary, receptors are detected in thecal and stromal cells and in follicular fluid. Ovarian CRH regulates ovarian steroidogenesis and is involved in follicular maturation, ovulation, and luteolysis. In this concise review we briefly discuss the role of ovarian CRH in reproduction, emphasizing its role in oocyte maturation.

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Corticotropin-releasing hormone inhibits in vitro oocyte maturation in mice

Cited by 13 publications

References 24 publications

The Effect of CRH and Its Inhibitor, Antalarmin, on in Vitro Growth of Preantral Mouse Follicles, Early Embryo Development, and Steroidogenesis

The Effect of CRH and Its Inhibitor, Antalarmin, on in Vitro Growth of Preantral Mouse Follicles, Early Embryo Development, and Steroidogenesis

MOLECULAR EVOLUTION OF GPCRS: CRH/CRH receptors

Update on the role of ovarian corticotropin‐releasing hormone

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