Cortisol assays and diagnostic laboratory procedures in human biological fluids

Gatti, Rosalba; Antonelli, Giorgia; Prearo, Maddalena; Spinella, Paolo; Cappellin, Enrico; Palo, Elio Franco De

doi:10.1016/j.clinbiochem.2009.04.011

Cited by 174 publications

(161 citation statements)

References 114 publications

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“…Other studies have detected cortisol in hair from dairy cows using radioimmunoassay with validation tests (Comin et al, 2011 andGonzález-de-la-Vara et al, 2011). Nevertheless, validation tests cannot be generalised or extrapolated from one technique to another or after using different extraction protocols (Buchanan and Goldsmith, 2004;Gatti et al, 2009;Stalder and Kirschbaum, 2012).…”

Section: Introductionmentioning

confidence: 99%

Hair cortisol detection in dairy cattle by using EIA: protocol validation and correlation with faecal cortisol metabolites

Talló-Parra

Manteca

Sabés-Alsina

et al. 2015

Animal

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Hair may be a useful matrix to detect cumulative cortisol concentrations in studies of animal welfare and chronic stress. The aim of this study was to validate a protocol for cortisol detection in hair from dairy cattle by enzyme immunoassay (EIA). Seventeen adult Holstein-Friesian dairy cows were used during the milking period. Hair cortisol concentration was assessed in 25-day-old hair samples taken from the frontal region of the head, analysing black and white coloured hair separately. Concentrations of cortisol metabolites were determined in faeces collected twice a week during the same period of time. There was a high correlation between cortisol values in faeces and cortisol in white colour hair samples but such correlation was not significant with the black colour hair samples. The intra-and inter-assay coefficients of variation were 4.9% and 10.6%, respectively. The linearity showed R 2 = 0.98 and mean percentage error of −10.8 ± 1.55%. The extraction efficiency was 89.0 ± 23.52% and the parallelism test showed similar slopes. Cortisol detection in hair by using EIA seems to be a valid method to represent long-term circulating cortisol levels in dairy cattle.Keywords: animal welfare, cortisol, dairy cattle, EIA, hair ImplicationsThe main implication of this study is the validation of a methodology for hair cortisol detection by enzyme immunoassay in dairy cattle. Hair cortisol detection is a promising methodology able to give information on long-term retrospective cortisol levels, which cannot be provided by any other established matrix. This study opens the possibility to use this methodology in chronic stress studies in dairy cattle welfare research, which is nowadays a hot topic.

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Section: Introductionmentioning

confidence: 99%

Hair cortisol detection in dairy cattle by using EIA: protocol validation and correlation with faecal cortisol metabolites

Talló-Parra

Manteca

Sabés-Alsina

et al. 2015

Animal

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“…The most commonly used assays to detect cortisol levels in these samples include radioimmunoassays (RIAs), liquid chromatography-mass spectrometry (LC-MS/MS) and enzyme-linked immunosorbent assays (ELISA) (Gatti et al, 2009). Although plasma samples provide a measurement of cortisol levels at a single point in time, they can only be used to assess acute stress, and are susceptible to physiological fluctuations.…”

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confidence: 99%

Social rank and cortisol among female rhesus macaques ( Macaca mulatta )

Qin¹,

Rizak²,

Feng³

et al. 2013

Zoological Research

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Abstract:In animal societies, some stressful events can lead to higher levels of physiological stress. Such stressors, like social rank, also predict an increased vulnerability to an array of diseases. However, the physiological relationship between social rank and stress varies between different species, as well as within groups of a single species. For example, dominant individuals are more socially stressed at times, while at other times it is the subordinate ones who experience this stress. Together, these variations make it difficult to assess disease vulnerability as connected to social interactions. In order to learn more about how physiological rank relationships vary between groups of a single species, cortisol measurements from hair samples were used to evaluate the effects of dominance rank on long-term stress levels in despotic and less stringent female rhesus macaque hierarchal groups. In despotic groups, cortisol levels were found not to be correlated with social rank, but a negative correlation was found between social rank and cortisol levels in less stringent hierarchies. Low ranking monkeys in less stringent groups secreted elevated levels of cortisol compared to higher ranking animals. These data suggest that variations in the strictness of the dominance hierarchy are determining factors in rank related stress physiology. The further consideration of nonhuman primate social system diversity and the linear degree of their hierarchies may allow for the development of valid rank-related stress models that will help increase our understanding and guide the development of new therapeutics for diseases related to human socioeconomic status.

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“…However, the basal and ACTH-stimulated FC levels of these patients remained within normal ranges (10). The measurement of the level of FC is technically difficult and expensive and is not routinely used (11). The level of FC can be calculated using the levels of TC and CBG (12).…”

Section: Introductionmentioning

confidence: 99%