Cortisol promotes stress tolerance via DAF-16 in Caenorhabditis elegans

Yasuda, Kensuke; Kubo, Yûki; Murata, Hiroki; Sakamoto, Kazuichi

doi:10.1016/j.bbrep.2021.100961

Cited by 7 publications

(8 citation statements)

References 31 publications

(29 reference statements)

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“…In N2 worms, nuclear DAF-16 accumulates in older but not in young animals [ 24 ]. The accumulation of DAF-16 is accelerated earlier under external stress conditions such as high temperature and oxidative stress [ 42 ]. This suggests that the activation of DAF-16 might be driven by aging.…”

Section: Discussionmentioning

confidence: 99%

Theanine Improves High-Dose Epigallocatechin-3-Gallate-Induced Lifespan Reduction in Caenorhabditis elegans

Peng

Dai

et al. 2021

Foods

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Epigallocatechin-3-gallate (EGCG) is the most abundant polyphenol in green tea. Our previous report showed that induced hormesis was a critical determinant for the promotion of a healthy lifespan in Caenorhabditis elegans. In the present study, we investigated the anti-aging effects of the main active ingredients in green tea. We found that galloylated catechins (EGCG and epicatechin gallate) could extend the lifespan of C. elegans, while their metabolites (gallic acid, epicatechin, and epigallocatechin) could not. Interestingly, the combination with theanine, not caffeine, could alleviate the adverse effects induced by high-dose EGCG, including the promotion of lifespan and locomotor ability. This was due to the attenuation of the excess production of reactive oxygen species and the activation of DAF-16. These findings will facilitate further studies on the health benefits of tea active components and their interactions.

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Section: Discussionmentioning

confidence: 99%

Theanine Improves High-Dose Epigallocatechin-3-Gallate-Induced Lifespan Reduction in Caenorhabditis elegans

Peng

Dai

et al. 2021

Foods

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“…Paratyphi A could significantly increase the nuclear localization of DAF-16 ( Figure 4C ; Supplementary Figure 2 ). We also analyzed the expression of daf-16 and its downstream target genes sod-3 , hsp-12.6 and dod-19 ( 44 ). The results indicated that S .…”

Section: Resultsmentioning

confidence: 99%

“…In addition, the results from transgenic strain TJ356 zIs356 [daf-16p::daf-16a/b:: GFP +rol-6(su1006)] indicated that S. Paratyphi A could significantly increase the nuclear localization of DAF-16 (Figure 4C; Supplementary Figure 2). We also analyzed the expression of daf-16 and its downstream target genes sod-3, hsp-12.6 and dod-19 (44). The results indicated that S. Paratyphi A significantly upregulated the expression of sod-3, hsp-12.6 and dod-19 (Figure 4D; Supplementary Table 12).…”

Section: Defense Against S Paratyphi a May Require The Participation ...mentioning

confidence: 91%

Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16

Ding

Zhang

et al. 2023

Front. Immunol.

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Salmonella enterica serovar Paratyphi A (S. Paratyphi A) is a pathogen that can cause enteric fever. According to the recent epidemic trends of typhoid fever, S. Paratyphi A has been the major important causative factor in paratyphoid fever. An effective vaccine for S. Paratyphi A has not been developed, which made it a tricky public health concern. Until now, how S. Paratyphi A interacts with organisms remain unknown. Here using lifespan assay, we found that S. Paratyphi A could infect Caenorhabditis elegans (C. elegans) at 25°C, and attenuate thermotolerance. The immune response of C. elegans was mediated by tir-1, nsy-1, sek-1, pmk-1, mpk-1, skn-1, daf-2 and daf-16, suggesting that S. Paratyphi A could regulate the MAPK and insulin pathways. Furthermore, we observed several phenotypical changes when C. elegans were fed S. Paratyphi A, including an accelerated decline in body movement, reduced the reproductive capacity, shortened spawning cycle, strong preference for OP50, arrested pharyngeal pumping and colonization of the intestinal lumen. The virulence of S. Paratyphi A requires living bacteria and is not mediated by secreting toxin. Using hydrogen peroxide analysis and quantitative RT-PCR, we discovered that S. Paratyphi A could increase oxidative stress and regulate the immune response in C. elegans. Our results sheds light on the infection mechanisms of S. Paratyphi A and lays a foundation for drugs and vaccine development.

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“…After 3 days of treatment, nematodes were transferred onto the fresh NGM plates (80 worms on each plate) and incubated at 35 °C for 10 h [ 48 ]. Numbers of survival nematodes were counted after the heat shock and the survival rate was calculated.…”

Section: Methodsmentioning

confidence: 99%

Novel Antioxidant Peptides Identified from Arthrospira platensis Hydrolysates Prepared by a Marine Bacterium Pseudoalteromonas sp. JS4-1 Extracellular Protease

et al. 2023

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Crude enzymes produced by a marine bacterium Pseudoalteromonas sp. JS4-1 were used to hydrolyze phycobiliprotein. Enzymatic productions showed good performance on DPPH radical and hydroxyl radical scavenging activities (45.14 ± 0.43% and 65.11 ± 2.64%, respectively), especially small peptides with MWCO <3 kDa. Small peptides were fractioned to four fractions using size-exclusion chromatography and the second fraction (F2) had the highest activity in hydroxyl radical scavenging ability (62.61 ± 5.80%). The fraction F1 and F2 both exhibited good antioxidant activities in oxidative stress models in HUVECs and HaCaT cells. Among them, F2 could upregulate the activities of SOD and GSH-Px and reduce the lipid peroxidation degree to scavenge the ROS to protect Caenorhabditis elegans under adversity. Then, 25 peptides total were identified from F2 by LC-MS/MS, and the peptide with the new sequence of INSSDVQGKY as the most significant component was synthetized and the ORAC assay and cellular ROS scavenging assay both illustrated its excellent antioxidant property.

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Cortisol promotes stress tolerance via DAF-16 in Caenorhabditis elegans

Cited by 7 publications

References 31 publications

Theanine Improves High-Dose Epigallocatechin-3-Gallate-Induced Lifespan Reduction in Caenorhabditis elegans

Theanine Improves High-Dose Epigallocatechin-3-Gallate-Induced Lifespan Reduction in Caenorhabditis elegans

Salmonella enterica serovar Paratyphi A-induced immune response in Caenorhabditis elegans depends on MAPK pathways and DAF-16

Novel Antioxidant Peptides Identified from Arthrospira platensis Hydrolysates Prepared by a Marine Bacterium Pseudoalteromonas sp. JS4-1 Extracellular Protease

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