Corynebacterium acnes in rheumatoid arthritis. I. Isolation and antibody studies.

Bartholomew, Lee E.; Nelson, Fred R.T.

doi:10.1136/ard.31.1.22

Cited by 20 publications

(11 citation statements)

References 26 publications

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“…Many species, e.g., P. acnes and S. epidermidis, were found in both the RA and non-RA patient groups, implying that their presence in synovium is not disease specific and that they are likely to be opportunistic colonizers of already diseased and compromised tissue. P. acnes is part of the normal skin microflora and has previously been isolated by culture from RA synovial fluid (6). Antigen from this organism has also been detected in synovial fluid leukocytes (7), implying an intracellular location.…”

Section: Discussionmentioning

confidence: 99%

Reverse Transcriptase-PCR Analysis of Bacterial rRNA for Detection and Characterization of Bacterial Species in Arthritis Synovial Tissue

Kempsell¹,

Cox

Hurle³

et al. 2000

Infect Immun

119

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Onset of rheumatoid arthritis (RA) is widely believed to be preceded by exposure to some environmental trigger such as bacterial infectious agents. The influence of bacteria on RA disease onset or pathology has to date been controversial, due to inconsistencies between groups in the report of bacterial species isolated from RA disease tissue. Using a modified technique of reverse transcriptase-PCR amplification, we have detected bacterial rRNA in the synovial tissue of late-stage RA and non-RA arthritis controls. This may be suggestive of the presence of live bacteria. Sequencing of cloned complementary rDNA (crDNA) products revealed a number of bacterial sequences in joint tissue from each patient, and from these analyses a comprehensive profile of the organisms present was compiled. This revealed a number of different organisms in each patient, some of which are common to both RA and non-RA controls and are probably opportunistic colonizers of previously diseased tissue and others which are unique species. These latter organisms may be candidates for a specific role in disease pathology and require further investigation to exclude them as causative agents in the complex bacterial millieu. In addition, many of the detected bacterial species have not been identified previously from synovial tissue or fluid from arthritis patients. These may not be easily cultivable, since they were not revealed in previous studies using conventional in vitro bacterial culture methods. In situ hybridization analyses have revealed the joint-associated bacterial rRNA to be both intra- and extracellular. The role of viable bacteria or their nucleic acids as triggers in disease onset or pathology in either RA or non-RA arthritis controls is unclear and requires further investigation.

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Section: Discussionmentioning

confidence: 99%

Reverse Transcriptase-PCR Analysis of Bacterial rRNA for Detection and Characterization of Bacterial Species in Arthritis Synovial Tissue

Kempsell¹,

Cox

Hurle³

et al. 2000

Infect Immun

119

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“…In addition there are reports of the isolation of diphtheroid-like organisms from tissue or blood of patients with rheumatoid arthritis (13,19). Because these reports are generally sporadic or unconfirmed, the differentiation of pathogenic from contaminant roles for isolated organisms is difficult.…”

Section: Discussionmentioning

confidence: 99%

Specific detection and semiquantitation of microorganisms in tissue by nucleic acid hybridization. II. Investigation of synovia from pigs with chronic erysipelothrix arthritis

Steinman

Hsu

1976

Arthritis & Rheumatism

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Synovial tissues from animals with chronic Erysipelothrix arthritis were examined by a technique based on RNA-DNA hybridization in an attempt to detect the inducing organism, Erysipelothrix insidiosa (EI). Twelve synovial specimens from 5 animals whose joints lacked culturable EI also lacked EI detectable by this technique. Because the technique is capable of detecting approximately one organism per 50 mammalian cells, it is comcluded that no more than this number were present in the involved tissue. Implications of these results for proposed pathogenetic mechanisms in this disorder and in human rheumatoid arthritis are discussed.In its chronic stage porcine Erysipelothrix arritis shares many features with human rheumatoid arthritis (1). The most notable difference is that a known bacterium, Erysipelothrix insidiosa (EI), is required to induce the animal disorder. But, as with rheumatoid

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“…P acnes was grown in BHI without serum as described previously (1). The washed organisms were heat-killed, sonicated, and extracted.…”

Section: Methodsmentioning

confidence: 99%

“…From the Division of Rheumatology, Department of Medi-We have previously reported the isolation of a phenol-water extractable antigen immunologically identical to a Proprionibacterium acnes antigen in rheumatoid (RA) synovial fluid and synovial leukocytes (1,2). Antigen was found only after extraction, suggesting that it is present in vivo in a form not available for immunologic identification.…”

Section: Antigenic Bacterial Polysaccharide In Rheumatoid Synovial Efmentioning

confidence: 99%

Antigenic bacterial polysaccharide in rheumatoid synovial effusions

Bartholomew

1979

Arthritis & Rheumatism

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Phenol-water extracted rheumatoid synovial fluids and synovial fluid leukocytes contain an antigen immunologically identical to the Proprionibucterium group bacteria. The antigen was identified by counterimmunoelectrophoresis in 70% of rheumatoid synovial fluid leukocyte pellets and in 60% of rheumatoid synovial fluids. It was also present in 6% of nonrhellmatoid fluids and in 22% of nonrheumatojd inflammatory fluid leukocytes. Antigen was not detectable in synovial samples before extraction. Synovial and bacterial antigens were further purified by proteolytic digestion and Sepharose 4B column chromatography. Biochemical and enzymatic studies of bacterial and synovial antigens were similar and consistent with a high molecular weight polysaccharide. Serum antibody to bacterial and synovial antigens was significantly less frequent in rheumatoid sera than in normal controls. The significance of demonstrating a bacterial polysaccharide primarily in rheumatoid synovial effusions is discussed.From the Division of Rheumatology, Department of MediWe have previously reported the isolation of a phenol-water extractable antigen immunologically identical to a Proprionibacterium acnes antigen in rheumatoid (RA) synovial fluid and synovial leukocytes (1,2). Antigen was found only after extraction, suggesting that it is present in vivo in a form not available for immunologic identification. Complement-fixing antibody to P ucnes was demonstrated in a significantly lower incidence of RA than control sera (1). This finding raised a number of possibilities including rapid utilization of antibody or a low level of recognition of antigen.This study reports a more sensitive assay method for detection of P acnes antigen in synovial fluid and synovial fluid leukocytes by counterimmunoelectrophoresis (CIE). With this system the frequency of isolation of P acnes antigen in RA samples is considerably higher than in our previous studies. Since free P acnes antigen has not been detected in synovial fluids or sera by CIE, a more sensitive method was studied by '251 radioautography.Antibody to P acnes antigen was also determined by CIE in sera and synovial fluid from patients with RA and various control groups. These results are compared Submitted for publication November 27, 1978; accepted in to our previous studies using a complement-fixation method for determination of antibody. The antigens have been partially purified by proteolytic digestion and Sepharose 4B chromatography. Biochemical and physical characteristics of the synovial and bacterial antigens were found to be similar. The immunologically reactive site is consistent with a high molecular weight polysaccharide, Proprionibacterium polysaccharide (PPS).

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Corynebacterium acnes in rheumatoid arthritis. I. Isolation and antibody studies.

Cited by 20 publications

References 26 publications

Reverse Transcriptase-PCR Analysis of Bacterial rRNA for Detection and Characterization of Bacterial Species in Arthritis Synovial Tissue

Reverse Transcriptase-PCR Analysis of Bacterial rRNA for Detection and Characterization of Bacterial Species in Arthritis Synovial Tissue

Specific detection and semiquantitation of microorganisms in tissue by nucleic acid hybridization. II. Investigation of synovia from pigs with chronic erysipelothrix arthritis

Antigenic bacterial polysaccharide in rheumatoid synovial effusions

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