Corynebacterium glutamicum as a potent biocatalyst for the bioconversion of pentose sugars to value-added products

Gopinath, Vipin; Anusree, Murali; Dhar, Kiran S.; Nampoothiri, K. Madhavan

doi:10.1007/s00253-011-3686-4

Cited by 37 publications

(11 citation statements)

References 83 publications

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“…d ‐ylose catabolism occurs via the d ‐xylose isomerase pathway in most bacteria, such as E . coli, or via the Weimberg pathway, e.g., in Caulobacter crescentus . Although the C. glutamicum genome has a xylulokinase gene ( NCgl0111 or xylB ) for the conversion of xylulose to xylulose‐5‐phosphate , d ‐xylose cannot be utilized as a carbon source owing to the low native xylB activity and lack of xylose isomerase, the first enzyme in the xylose isomerase pathway, which mediates the conversion of d ‐xylose into xylulose .…”

Section: Utilization Of Alternative Carbon Sources For the Productionmentioning

confidence: 99%

“…To establish d ‐xylose utilization in C. glutamicum , heterologous expression of xylose isomerase is required for the conversion of xylose to xylulose (Fig. ) . Heterologous expression of xylose isomerase ( xylA ) from Xanthomonas campestris resulted in a faster growth rate and higher biomass concentration compared to those observed using the xylA gene from E. coli (0.14/h, 3.37 gCDW L −1 > 0.09/h, 2.79 gCDW L −1 ) .…”

Section: Utilization Of Alternative Carbon Sources For the Productionmentioning

confidence: 99%

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Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Baritugo

Kim

David

et al. 2018

Biofuels Bioprod Bioref

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The fermentative production of platform chemicals in biorefineries is a sustainable alternative to current petroleum‐refining processes. Industrial microorganisms, such as Escherichia coli, Saccharomyces cerevisiae, and Corynebacterium glutamicum, have been engineered as microbial cell factories that are able to utilize biomass for the production of value‐added platform chemicals and polymers. Compared to E. coli and S. cerevisiae, C. glutamicum displays weak carbon catabolite repression and can co‐utilize mixed sugars as carbon sources, without any significant growth retardation. Pathways for the utilization of alternative carbon sources, such as d‐xylose and l‐arabinose from lignocellulosic biomass, lactose and galactose from whey, glycerol from biodiesel, and methanol from natural gas refineries, have been evaluated for chemical production. However, the application of C. glutamicum in biorefineries is limited because it does not secrete hydrolases for the efficient utilization of cellulose, xylan, and starch from lignocellulosic and starch biomass. To solve the limitation, C. glutamicum has been engineered for the consolidated bioprocessing of biomass by the heterologous expression of amylolytic and cellulolytic enzymes. Recently, C. glutamicum has been extensively engineered for polyamide monomer production owing to its ability to produce l‐lysine and l‐glutamate. This review summarizes recent advances in the development of C. glutamicum strains that can utilize renewable biomass resources for the production of industrially important chemicals. It highlights recent progress in metabolic engineering for the production of polyamide monomers. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

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Section: Utilization Of Alternative Carbon Sources For the Productionmentioning

confidence: 99%

Section: Utilization Of Alternative Carbon Sources For the Productionmentioning

confidence: 99%

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Baritugo

Kim

David

et al. 2018

Biofuels Bioprod Bioref

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“…This microorganism is widely used for the production of various amino acids and other industrially relevant compounds [1,2]. Furthermore, the availability of genetic engineering methods, an easy cultivation and a generally-regarded-as-safe status has helped to make it a model organism for systems biology investigations in the Corynebacterineae , comprising important pathogens such as Corynebacterium diphtheriae and Mycobacterium tuberculosis [3] .…”

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of the Corynebacterium glutamicum transcriptome using an improved RNAseq technique

et al. 2013

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BackgroundThe use of RNAseq to resolve the transcriptional organization of an organism was established in recent years and also showed the complexity and dynamics of bacterial transcriptomes. The aim of this study was to comprehensively investigate the transcriptome of the industrially relevant amino acid producer and model organism Corynebacterium glutamicum by RNAseq in order to improve its genome annotation and to describe important features for transcription and translation.ResultsRNAseq data sets were obtained by two methods, one that focuses on 5′-ends of primary transcripts and another that provides the overall transcriptome with an improved resolution of 3′-ends of transcripts. Subsequent data analysis led to the identification of more than 2,000 transcription start sites (TSSs), the definition of 5′-UTRs (untranslated regions) for annotated protein-coding genes, operon structures and many novel transcripts located between or in antisense orientation to protein-coding regions. Interestingly, a high number of mRNAs (33%) is transcribed as leaderless transcripts. From the data, consensus promoter and ribosome binding site (RBS) motifs were identified and it was shown that the majority of genes in C. glutamicum are transcribed monocistronically, but operons containing up to 16 genes are also present.ConclusionsThe comprehensive transcriptome map of C. glutamicum established in this study represents a major step forward towards a complete definition of genetic elements (e.g. promoter regions, gene starts and stops, 5′-UTRs, RBSs, transcript starts and ends) and provides the ideal basis for further analyses on transcriptional regulatory networks in this organism. The methods developed are easily applicable for other bacteria and have the potential to be used also for quantification of transcriptomes, replacing microarrays in the near future.

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“…This microorganism has a long history of applications in the production of various amino acids and other industrially relevant compounds [1, 2]. Furthermore, it serves as a model organism for close relatives with medical significance such as C. diphtheriae or Mycobacterium tuberculosis .…”

Section: Introductionmentioning

confidence: 99%

Comprehensive discovery and characterization of small RNAs in Corynebacterium glutamicumATCC 13032

et al. 2013

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BackgroundRecent discoveries on bacterial transcriptomes gave evidence that small RNAs (sRNAs) have important regulatory roles in prokaryotic cells. Modern high-throughput sequencing approaches (RNA-Seq) enable the most detailed view on transcriptomes offering an unmatched comprehensiveness and single-base resolution. Whole transcriptome data obtained by RNA-Seq can be used to detect and characterize all transcript species, including small RNAs. Here, we describe an RNA-Seq approach for comprehensive detection and characterization of small RNAs from Corynebacterium glutamicum, an actinobacterium of high industrial relevance and model organism for medically important Corynebacterianeae, such as C. diphtheriae and Mycobacterium tuberculosis.ResultsIn our RNA-Seq approach, total RNA from C. glutamicum ATCC 13032 was prepared from cultures grown in minimal medium at exponential growth or challenged by physical (heat shock, cold shock) or by chemical stresses (diamide, H2O2, NaCl) at this time point. Total RNA samples were pooled and sequencing libraries were prepared from the isolated small RNA fraction. High throughput short read sequencing and mapping yielded over 800 sRNA genes. By determining their 5′- and 3′-ends and inspection of their locations, these potential sRNA genes were classified into UTRs of mRNAs (316), cis-antisense sRNAs (543), and trans-encoded sRNAs (262). For 77 of trans-encoded sRNAs significant sequence and secondary structure conservation was found by a computational approach using a whole genome alignment with the closely related species C. efficiens YS-314 and C. diphtheriae NCTC 13129. Three selected trans-encoded sRNAs were characterized by Northern blot analysis and stress-specific transcript patterns were found.ConclusionsThe study showed comparable numbers of sRNAs known from genome-wide surveys in other bacteria. In detail, our results give deep insight into the comprehensive equipment of sRNAs in C. glutamicum and provide a sound basis for further studies concerning the functions of these sRNAs.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-14-714) contains supplementary material, which is available to authorized users.

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Corynebacterium glutamicum as a potent biocatalyst for the bioconversion of pentose sugars to value-added products

Cited by 37 publications

References 83 publications

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Comprehensive analysis of the Corynebacterium glutamicum transcriptome using an improved RNAseq technique

Comprehensive discovery and characterization of small RNAs in Corynebacterium glutamicumATCC 13032

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