Corynebacterium parvum as the Priming Agent in the Production of Tumor Necrosis Factor in the Mouse2

Green, Saul; Dobrjansky, Areta; Chiasson, Mary Ann; Carswell, Elizabeth A.; Schwartz, Morton K.; Old, Lloyd J.

doi:10.1093/jnci/59.5.1519

Cited by 119 publications

(64 citation statements)

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“…Preparation of the sera Sera were prepared as described by Green, Dobrjansky, Chiasson, Carswell, Schwartz & Old (1977) in female Swiss random mice obtained from the Central Institute for the Breeding of Laboratory Animals (CPB, Zeist, The Netherlands). TNS was designated according to the inducing and eliciting agents.…”

Section: Methodsmentioning

confidence: 99%

In vitro anti-tumour activity of tumour necrosis serum

Bloksma

Schetters

Figdor

et al. 1980

International Journal of Immunopharmacology

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Abstract--A method measuring 3H-thymidine incorporation in Meth A sarcoma cells was used to quantify in vitro anti-tumour activity of tumour necrosis serum and compared with a method using cell viability as a parameter. Tumour necrosis serum obtained from mice pretreated with Corynebacterium parvum and elicited with endotoxin two weeks later greatly inhibited 3H-thymidine incorporation, whereas sera of normal mice and of mice treated with C. parvum or endotoxin alone were much less inhibitory. All sera reduced viable cell numbers, tumour necrosis serum being most active.The 3H-thymidine incorporation assay is suited for screening mouse sera on anti-tumour activity. It was shown that the anti-tumour activity of normal mouse serum can be potentiated by in vivo pretreatment of mice with bacterial agents. The mechanism of the anti-tumour action(s) and the factor(s) involved remain to be elucidated.

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Section: Methodsmentioning

confidence: 99%

In vitro anti-tumour activity of tumour necrosis serum

Bloksma

Schetters

Figdor

et al. 1980

International Journal of Immunopharmacology

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“…The degree of splenomegaly was shown to parallel the antitumor activity of P. acnes (11). Furthermore, P. acnes-treated mice develop a state of hypersensitivity to bacterial LPS (12,13). The maximum LPS sensitization is reached on day 7 after P. acnes administration.…”

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confidence: 99%

Requirement for TLR9 in the Immunomodulatory Activity of Propionibacterium acnes

Kalis

Gumenscheimer

Freudenberg

et al. 2005

The Journal of Immunology

118

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Propionibacterium acnes (formerly Corynebacterium parvum) is part of the human flora and, as such, is associated with several human pathologies. It possesses strong immunomodulatory activities, which makes this bacterium interesting for prophylactic and therapeutic vaccination. The bacterial component(s) and the host receptor(s) involved in the induction of these activities are poorly understood. We show in this study that TLR9 is crucial in generating the characteristic effects of killed P. acnes priming in the spleen, such as extramedullary hemopoiesis and organ enlargement, and granuloma formation in the liver. Furthermore, the ability to overproduce TNF-α and IFN-γ in response to LPS, lipid A, synthetic lipopeptide Pam3CysK4, or whole killed bacteria was present in P. acnes-primed wild-type, but not TLR9−/−, mice. Finally, P. acnes priming failed to induce enhanced resistance to murine typhoid fever in TLR9−/− mice. Thus, TLR9 plays an essential role in the induction of immunomodulatory effects by P. acnes. Because IFN-γ is a key mediator of these effects, and enhanced IFN-γ mRNA expression was absent in spleen and liver of P. acnes-primed TLR9−/− mice, we conclude that TLR9 is required for the induction of IFN-γ by P. acnes.

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“…with a dose of LTA or LPS. Table III shows that the pretreatment with P. acnes caused heightened susceptibility to endotoxin lethality in mice like that reported in C. parvumprimed mice (Benacerraf et al, 1959;Green et al, 1977). Two out of the 4 mice were killed by the injection of 0.8 pg of LPS and all the mice receiving 3.13pg or more died within 6h.…”

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confidence: 52%

“…However, the results of several groups have indicated that LPS is the only successful agent for eliciting TNF in primed animals (Green et al, 1977;Mannel et al, 1980;Parant et al, 1980). From the practical point of view, however, the use of endotoxin to induce TNF may be limited by its strong undesirable side effects due to high toxicity and pyrogenicity.…”

mentioning

confidence: 99%

“…The in vitro cytotoxic effect of TNF is assumed to be due to the same factor as that which causes haemorrhagic necrosis of tumour in vivo (Green et al, 1977, Ostrove & Gifford, 1979. For the cytotoxic test in vitro, a suspension of L-929 cells (5 x 104 cellsml-1) in RPMI-1640 medium supplemented with 10% FCS was distributed in a microplate having 24 wells (0.5 ml/well), and the cells were incubated for 3 h at 37°C in 5% CO2 in air.…”

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confidence: 99%

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