Corynebacterium sundsvallense sp. nov., from human clinical specimens

Abstract

Three strains of a previously undescribed catalase-positive non-lipophilic coryneform bacterium isolated from human clinical specimens were characterized by phenotypic and molecular taxonomic methods. Morphologically the unknown bacterium consisted of pleomorphic rods, some of which displayed bulgesknobs a t their ends. All three strains were similar in that they produced acid from fructose, glucose, maltose and sucrose and were urease-positive. Chemotaxonomic investigations revealed the presence of meso-diaminopimelic acid and short-chain mycolic acids consistent with the genus Corynebacterium sensu stricto. Comparative 165 rRNA gene sequencing showed that the three strains are genealogically highly related and constitute a new subline within the genus Corynebacterium, displaying > 3 O/ O sequence divergence with recognized species. The unknown bacterium was distinguished from currently validly published Corynebacteriurn species by phenotypic tests, including electrophoretic analysis of whole-cell proteins. Based on phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium from clinical specimens be classified as Corynebacterium sundsvallense sp. nov. The type strain is CCUG 36622T.

Corynebacterium simulans sp. nov., a non-lipophilic, fermentative Corynebacterium.

“…Most of them have been revised by Funke et al (1997a). More recently, additional species have been described (Fernandez-Garayzabal et al, 1997Funke et al, 1997bFunke et al, , c, d, e, 1998aCollins et al, 1998Collins et al, , 1999Pascual et al, 1998 ;Riegel et al, 1997a, b ;Takeuchi et al, 1999 ;Zimmermann et al, 1998). Using recently developed identification tests (Wauters et al, 1998), three strains were isolated from human clinical samples displaying identical but atypical phenotypic and metabolic profiles.…”

Corynebacterium freneyi sp. nov., alpha-glucosidase-positive strains related to Corynebacterium xerosis.

“…Corynebacterium pseudotuberculosis, which possesses α-glucosidase in one case out of two, is of veterinary origin and is distinguished from C. freneyi by the presence of urease and the absence of pyrazinamidase. Corynebacterium sundsvallense, a recently described α-glucosidase-positive strain, hydrolyses urea (Collins et al, 1999).…”

Nondiphtherial Corynebacterium species isolated from clinical specimens of patients in a university hospital, Rio de Janeiro, Brazil

“…During the last decade, new species isolated from humans have been described: Corynebacterium auris, Corynebacterium argentoratense, Corynebacterium durum, Corynebacterium imitans, Corynebacterium mucifaciens, Corynebacterium lipophiloflavum, Corynebacterium coyleae, Corynebacterium riegelii (10), Corynebacterium confusum, Corynebacterium thomssenii (10,12), Corynebacterium falsenii (10,20), Corynebacterium kroppenstedtii and Corynebacterium sundsvallense (2,12).…”