Cosmetic Improvement in Various Acute Skin Defects Treated With Tissue‐engineered Skin

Abstract

Acute skin defects often cause many adverse events such as abnormal pigmentation and scar formation, the satisfactory healing of which remains a significant clinical challenge. Over the past several decades, a number of skin equivalents have been available for clinical purposes to promote wound closure. However, the true values of skin equivalent - tissue-engineered skin (TE-skin) composed of neonatal fibroblasts and keratinocytes - in improving the quality of wound healing are not yet elucidated. A total of 158 patients were enrolled, 129 of which were used in this study. In these patients, acute skin defects were treated with TE-skin as experimental group, and treated with Vaseline primary dressing as control group. The differences in average healing times between the two groups were determined with statistical analysis according to different depths of skin defects. Wound quality, including pigmentation, cicatrization, and pliability, was assessed by investigators from different clinical centers over a 6-month period. The cosmetic outcome of the wound was further evaluated with histological method. In the study, the average time of wound closure in the experimental group was significantly shortened by 6.5 to 20 days according to different depths of skin defects. The cosmetic quality of reconstructed skin was satisfactory, with the patients enjoying better pliability, less abnormal pigmentation, and cicatrization. Safety analysis demonstrated that the wounds treated with TE-skin did not show clinical or laboratory evidence of rejection during the trial. These results indicate that TE-skin is a suitable and clinically effective treatment for various acute skin defects. Furthermore, the TE-skin appears to produce more satisfactory cosmetic results when compared with the conventional therapy.

Características biológicas das células-tronco mesenquimais

“…78 Na dermatologia, as células-tronco têm sido avaliadas na reposição epitelial de áreas de queimadura, na cicatrização de feridas crônicas e no tratamento do vitiligo, entre outros. 80,81 Um dos principais alvos de pesquisas, já em desenvolvimento, com células-tronco encontra-se na área de oncologia e, principalmente a onco-hematologia. 82 A utilização de células-tronco, nestes casos, estaria relacionada com a reconstrução dos tecidos.…”

Reprogramming human adipose tissue stem cells using epidermal keratinocyte extracts

“…One major limiting factor of autologous skin grafts is the availability of autologous skin. In recent years, there have been significant advancements in tissue-engineered skin replacements ( 1 – 3 ); however, a good source of autogeneic cells remains to be determined. Nuclear reprogramming offers a promising method, whereby extracts from a specific type of cell are introduced into donor cells and the donor cells subsequently acquire the characteristics of the cells from which the extracts were derived ( 4 ).…”

Revista Tecnología en Marcha. Número Especial Ingeniería de Tejidos. Mayo 2015

“…Además, se han reportado disminuciones en el tiempo de cicatrización y la hiperpigmentación e hipopigmentación, así como mejorías en la flexibilidad y la calidad de la cicatrización, sin reacciones inmunológicas adversas por parte del paciente receptor. Inicialmente, estos sustitutos fueron desarrollados como vendajes; recientemente se han diseñado para estimular la reepitelización y la formación de piel nueva, gracias a la secreción de factores de crecimiento (Nie et al, 2007). Los productos comerciales de este tipo (Apligraf® y OrCel®) no han reportado evidencia de rechazo inmunológico.…”