Cosmopolitan distribution of phlD-containing dicotyledonous crop-associated biocontrol pseudomonads of worldwide origin

Wang, C

doi:10.1016/s0168-6496(01)00151-9

Cited by 30 publications

(58 citation statements)

References 35 publications

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“…decline soils (Raaijmakers et al, 1997;Raaijmakers and Weller, 1998;McSpadden-Gardener et al, 2000;Weller et al, 2002;Souza et al, 2003a) and for soils suppressive to black root rot of tobacco (Wang et al, 2001;Ramette et al, 2003Ramette et al, , 2006Frapolli et al, 2007). This strategy encompasses a comparative analysis of the densities, diversity and activity of antibiotic-producing pseudomonads in two soils chosen for their opposite level of suppressiveness to Fusarium wilt: one being suppressive (Châteaur-enard) and the other being conducive (Carquefou).…”

Section: Discussionmentioning

confidence: 99%

“…Also the antibiotic 2,4-diacetylphloroglucinol (DAPG) has substantial activity against pathogenic F. oxysporum (Schouten et al, 2004), and populations of DAPG-producing pseudomonads were highly enriched in a soil naturally suppressive to Fusarium wilt of peas (Landa et al, 2002). Following similar molecular-based approaches used to unravel the role of antibiosis in take-all decline soils (Raaijmakers et al, 1997;Raaijmakers and Weller, 1998;McSpadden-Gardener et al, 2000;Weller et al, 2002;Souza et al, 2003a) and in soils suppressive to black root rot of tobacco (Wang et al, 2001;Ramette et al, 2003Ramette et al, , 2006Frapolli et al, 2007), the objectives of this study were to determine the frequency, diversity and biocontrol activity of DAPG (phlD þ )-and phenazine (phzC þ )-producing pseudomonads in the Fusarium-wilt suppressive soil from Châteaurenard (France) in comparison with the conducive soil of Carquefou (France). The results showed that densities of phlD þ populations were not significantly different between the two soils but that their genotypic diversity differed significantly.…”

Section: Introductionmentioning

confidence: 99%

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Phenazine antibiotics produced by fluorescent pseudomonads contribute to natural soil suppressiveness to Fusarium wilt

et al. 2009

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Natural disease-suppressive soils provide an untapped resource for the discovery of novel beneficial microorganisms and traits. For most suppressive soils, however, the consortia of microorganisms and mechanisms involved in pathogen control are unknown. To date, soil suppressiveness to Fusarium wilt disease has been ascribed to carbon and iron competition between pathogenic Fusarium oxysporum and resident non-pathogenic F. oxysporum and fluorescent pseudomonads. In this study, the role of bacterial antibiosis in Fusarium wilt suppressiveness was assessed by comparing the densities, diversity and activity of fluorescent Pseudomonas species producing 2,4-diacetylphloroglucinol (DAPG) (phlD+) or phenazine (phzC+) antibiotics. The frequencies of phlD+ populations were similar in the suppressive and conducive soils but their genotypic diversity differed significantly. However, phlD genotypes from the two soils were equally effective in suppressing Fusarium wilt, either alone or in combination with non-pathogenic F. oxysporum strain Fo47. A mutant deficient in DAPG production provided a similar level of control as its parental strain, suggesting that this antibiotic does not play a major role. In contrast, phzC+ pseudomonads were only detected in the suppressive soil. Representative phzC+ isolates of five distinct genotypes did not suppress Fusarium wilt on their own, but acted synergistically in combination with strain Fo47. This increased level of disease suppression was ascribed to phenazine production as the phenazine-deficient mutant was not effective. These results suggest, for the first time, that redox-active phenazines produced by fluorescent pseudomonads contribute to the natural soil suppressiveness to Fusarium wilt disease and may act in synergy with carbon competition by resident non-pathogenic F. oxysporum.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phenazine antibiotics produced by fluorescent pseudomonads contribute to natural soil suppressiveness to Fusarium wilt

et al. 2009

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“…Clones of other soil microbes (Pseudomonas spp.) have also been shown to occur over wide geographic areas [30,43,47,71]. Clones that were identified from 16S sequences as belonging to OTU 1 were found across locations.…”

Section: Discussionmentioning

confidence: 99%

Spatial Variation in Streptomyces Genetic Composition and Diversity in a Prairie Soil

et al. 2004

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Understanding how microbial genotypes are arrayed in space is crucial for identifying local factors that may influence the spatial distribution of genetic diversity. In this study we investigated variation in 16S rDNA sequences and rep-PCR fingerprints of Streptomyces stains isolated from prairie soil among three locations and four soil depths. Substantial variation in Streptomyces OTU (operational taxonomic unit) and BOX-PCR fingerprint diversity was found among locations within a limited spatial area (1 m 2 ). Further, phylogenetic lineages at each location were distinct. However, there was little variation in genetic diversity among isolates from different soil depths and similar phylogenetic lineages were found at each depth. Some clones were found at a localized scale while other clones had a relatively widespread distribution. There was poor correspondence between 16S rDNA groupings and rep-PCR fingerprint groupings. The finding of distinct phylogenetic lineages and the variation in spatial distribution of clones suggests that selection pressures may vary over the soil landscape.

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“…Bacteria were characterized on the basis of carbon-source usage with the API 50 CHE kit (BioMérieux, Marcy l'Etoile, France) and 16S rDNA sequencing (Amodia GmbH, Braunschweig, Germany). For verification of bacterial isolates during experiments, polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) was employed as described previously (Wang et al 2001), with minor modifications. Bacterial lysates were diluted 50-fold in sterile water and 4 l was used in a 50-l PCR with redtaq (SigmaAldrich) to amplify the ITS of the rDNA region with the following primers: 16Sifwd (5 -TTGTACACACCGCCCGTC A-3 ) and 23Sirev (5 -GGTACCTTAGATGTTTCAGTTC-3 ).…”

Section: Characterization Of Oxalate-degrading Bacteriamentioning

confidence: 99%

Oxalate-Degrading Bacteria Can ProtectArabidopsis thalianaand Crop Plants AgainstBotrytis cinerea

Schoonbeek¹,

Jacquat-Bovet²,

Mascher³

et al. 2007

MPMI

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Botrytis cinerea and Sclerotinia sclerotiorum secrete oxalic acid as a pathogenicity factor with a broad action. Consequently, it should be possible to interfere with the infection process by degrading oxalic acid during the interaction of these pathogens with their hosts. We have evaluated the potential of oxalate-degrading bacteria to protect plants against pathogenic fungi. Such bacteria were isolated from agricultural soil and selected on agar plates with Ca-oxalate as the sole carbon source. Four strains were retained with a medium-to-strong protective activity on Arabidopsis thaliana leaves against B. cinerea and S. sclerotiorum. They can provide 30 to 70% protection against fungal infection in different pathosystems, including B. cinerea on A. thaliana, cucumber, grapevine, and tomato. The oxalatedegrading bacteria induced only some marker genes for common plant signaling pathways for defenses, but protective effects were slightly reduced in A. thaliana mutants impaired in the ethylene and jasmonic acid signaling pathways. More detailed studies on the protective mechanism were performed in ox-strain B, identified as Cupriavidus campinensis, by analysis of transposon-tagged mutants that have a reduced ability to degrade oxalic acid.

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Cosmopolitan distribution of phlD-containing dicotyledonous crop-associated biocontrol pseudomonads of worldwide origin

Cited by 30 publications

References 35 publications

Phenazine antibiotics produced by fluorescent pseudomonads contribute to natural soil suppressiveness to Fusarium wilt

Phenazine antibiotics produced by fluorescent pseudomonads contribute to natural soil suppressiveness to Fusarium wilt

Spatial Variation in Streptomyces Genetic Composition and Diversity in a Prairie Soil

Oxalate-Degrading Bacteria Can ProtectArabidopsis thalianaand Crop Plants AgainstBotrytis cinerea

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