2021
DOI: 10.3390/s21216945
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Cost-Effective Multiplex Fluorescence Detection System for PCR Chip

Abstract: The lack of portability and high cost of multiplex real-time PCR systems limits the device to be used in POC. To overcome this issue, this paper proposes a compact and cost-effective fluorescence detection system that can be integrated to a multiplex real-time PCR equipment. An open platform camera with embedded lens was used instead of photodiodes or an industrial camera. A compact filter wheel using a sliding tape is integrated, and the excitation LEDs are fixed at a 45° angle near the PCR chip, eliminating … Show more

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Cited by 6 publications
(3 citation statements)
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“…34,35 However, they have some technical limitations such as additional lens attachment, different optical paths, and mechanical replacement of fluorescence detection elements for multi-target diagnostics. 36 Microlens array configurations allow close-up fluorescence imaging and real-time quantification during rapid plasmonic PCR at the handheld level but still require simultaneous multi-color quantification in the small RT-qPCR system. 27,37,38 Here, we report a single-shot multi-channel plasmonic RT-qPCR (mpRT-qPCR) assay with ultrafast nanostructure-driven amplification and multi-target quantification.…”
Section: Introductionmentioning
confidence: 99%
“…34,35 However, they have some technical limitations such as additional lens attachment, different optical paths, and mechanical replacement of fluorescence detection elements for multi-target diagnostics. 36 Microlens array configurations allow close-up fluorescence imaging and real-time quantification during rapid plasmonic PCR at the handheld level but still require simultaneous multi-color quantification in the small RT-qPCR system. 27,37,38 Here, we report a single-shot multi-channel plasmonic RT-qPCR (mpRT-qPCR) assay with ultrafast nanostructure-driven amplification and multi-target quantification.…”
Section: Introductionmentioning
confidence: 99%
“…In the above isothermal amplification methods, as for traditional qPCR, DNA is detected and quantified by monitoring a change in fluorescence intensity. 25–29 This requires fluorescent labelling reagents as well as a fluorescence excitation and detection device, which increases the complexity and is not favorable to the needs of on-site detection.…”
Section: Introductionmentioning
confidence: 99%
“…D ETECTION of fluorescence with a configuration consisted of excitation light source, micro-fluidic chip, optical filters, and complementary metal-oxide-semiconductor (CMOS) sensors, plays a key role in the quantitative analysis of nucleic acid by polymerase chain reaction (PCR) [1], [2], [3], [4], [5], [6], [7]. In this special era that the COVID-19 has been threatening public health all over the world for almost 3 years since the end of year 2019, a rapid responding, portable, and high Wenbing Li is with the Shanghai Industrial µTechnology Research Institute, Shanghai 201899, China (e-mail: quentinlee08@foxmail.com).…”
Section: Introductionmentioning
confidence: 99%