Cost-effective optimized scleroglucan production by Sclerotium rolfsii ATCC 201126 at bioreactor scale. A quantity-quality assessment

Valdez, Alejandra Leonor; Delgado, Osvaldo Daniel; Fariña, Julia Inés

doi:10.1016/j.carbpol.2020.117505

Cited by 7 publications

(5 citation statements)

References 24 publications

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“…cesses [30][31][32][33]. Under these conditions, the Reynolds number was Re = 5.2 10 3 , which indicated operation in the turbulent flow regime [34], so that a reasonable estimation of local quantities can be obtained from 2D measurements.…”

Section: Parametermentioning

confidence: 90%

“…For all experiments, the rotational speed N was 6.66 rps (400 rpm), and the air flow rate was fixed at Q = 2 L >min −1 , equivalent to 0.25 volume of gas per volume of liquid per minute (vvm). Such conditions were based on the experimental works reported elsewhere for applications in fermentation processes 30–33. Under these conditions, the Reynolds number was Re = 5.2 × 10 3 , which indicated operation in the turbulent flow regime 34, so that a reasonable estimation of local quantities can be obtained from 2D measurements.…”

Section: Methodsmentioning

confidence: 99%

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Flow Patterns of Multiple Axial‐Radial Impellers for Potential Use in Aerated Stirred Tanks

et al. 2022

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The hydrodynamics of a stirred tank equipped with multiple hybrid impellers was experimentally characterized. They were coaxially placed to agitate a viscous Newtonian fluid under gassed conditions. Experiments were performed by using the particle image velocimetry technique, and flow fields were reported in terms of the velocity magnitude, vertical currents, turbulent intensity, and strain rates. For comparison purposes, impellers providing radial flow (Rushton turbines) and axial flow (pitched blade turbines) were also tested. The results showed that hybrid impellers provided a uniform flow distribution throughout the tank at moderate strain rates, which make them an attractive alternative for fermentation processes with shear-sensitive cultures and viscous liquids.

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Section: Parametermentioning

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Flow Patterns of Multiple Axial‐Radial Impellers for Potential Use in Aerated Stirred Tanks

et al. 2022

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“…Castillo, Valdez, & Fariña, 2015;Geller & Yan, 2020;Jindal & Singh Khattar, 2018;Kırtel, Avşar, Erkorkmaz, & Öner, 2017;Subhadip Mahapatra & Debdulal Banerjee, 2013a;Microbial Polymers, Applications, and Ecological Perspectives, 2021;PubChem, 2021b;J. Song et al, 2020;Valdez, Delgado, & Fariña, 2021)…”

Section: Extraction Methods Of Fungal Epssmentioning

confidence: 99%

Fungal exopolysaccharides: Properties, sources, modifications, and biomedical applications

Hamidi

Okoro

Milan

et al. 2022

Carbohydrate Polymers

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“…Scleroglucan is a microbial exopolysaccharide, and is a typical β-glucan (Bai et al, 2021;Valdez et al, 2021). It consists of backbone β-1,3-linked-glucopyranosyl residues and branches of β-1,6-linkedglucopyranosyl residues.…”

Section: Introductionmentioning

confidence: 99%

“…xylose and molasses (Survase et al, 2007b;Taskin et al, 2010;Leonor Valdez et al, 2019). Strategies have been introduced to enhance its production, most of which were focused on the selection of medium components and cost-effective optimization of controlling fermentation conditions (Survase et al, 2007a;Zhang et al, 2017;Valdez et al, 2021). In our previous research, 42.0 g/L of scleroglucan was obtained with the screened strain S. rolfsii WSH-G01 (Tan et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Efficient Production of Scleroglucan by Sclerotium rolfsii and Insights Into Molecular Weight Modification by High-Pressure Homogenization

Zeng

Wang

Shan

et al. 2021

Front. Bioeng. Biotechnol.

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Scleroglucan is a non-ionic water-soluble polysaccharide, and has been widely used in the petroleum, food, medicine and cosmetics industries. Currently, scleroglucan is mainly produced by Sclerotium rolfsii. A higher level of scleroglucan (42.0 g/L) was previously obtained with S. rolfsii WSH-G01. However, the production of scleroglucan was reduced despite a higher glucose concentration remaining. Additionally, the molecular weight of scleroglucan was large, thus restricted its application. In this study, by adjusting the state of seeds inoculated, the degradation issue of scleroglucan during the fermentation process was solved. By comparing different fed-batch strategies, 66.6 g/L of scleroglucan was harvested by a two-dose fed-batch mode, with 53.3% glucose conversion ratio. To modify the molecular weight of scleroglucan, a combination method with HCl and high-pressure homogenization treatment was established. Finally, scleroglucan with molecular weight of 4.61 × 105 Da was obtained. The developed approaches provide references for the biosynthesis and molecular weight modification of polysaccharides.

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Cost-effective optimized scleroglucan production by Sclerotium rolfsii ATCC 201126 at bioreactor scale. A quantity-quality assessment

Cited by 7 publications

References 24 publications

Flow Patterns of Multiple Axial‐Radial Impellers for Potential Use in Aerated Stirred Tanks

Flow Patterns of Multiple Axial‐Radial Impellers for Potential Use in Aerated Stirred Tanks

Fungal exopolysaccharides: Properties, sources, modifications, and biomedical applications

Efficient Production of Scleroglucan by Sclerotium rolfsii and Insights Into Molecular Weight Modification by High-Pressure Homogenization

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