Cost-Efficient Production of the Sphingan WL Gum by Sphingomonas sp. WG Using Molasses and Sucrose as the Carbon Sources

Liu, Jianlin; Li, Hui; Zhang, Xuanyu; Yue, Lin; Lu, Wei; Ma, Sen; Zhu, Zhiming; Wang, Dong; Zhu, Hu; Wang, Jiqian

doi:10.1007/s10126-022-10193-1

Cited by 4 publications

(1 citation statement)

References 44 publications

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“…Bacterial polysaccharides have the potential to be used in a range of applications, but few have found widespread use. Current production pipelines rely on extracting polysaccharides directly from producing organisms (10)(11)(12). This approach restricts the potential of glycans that are produced at lower quantities or by organisms that are not amenable to large scale fermentation.…”

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A novel exopolysaccharide pathway from a freshwaterSphingomonasisolate

Goetsch,

Ufearo,

Keiser

et al. 2023

Preprint

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Bacteria embellish their cell envelopes with a variety of specialized polysaccharides. Biosynthesis pathways for these glycans are complex, and final products vary greatly in their chemical structures, physical properties and biological activities. This tremendous diversity comes from the ability to arrange complex pools of monosaccharide building blocks into polymers with many possible linkage configurations. Due to the complex chemistry of bacterial glycans, very few biosynthetic pathways have been defined in detail. To better understand the breadth of polysaccharide production in nature we isolated a bacterium from Lake Michigan calledSphingomonas sp.LM7 that is proficient in exopolysaccharide (EPS) production. We identified genes that contribute to EPS biosynthesis in LM7 by screening a transposon mutant library for colonies displaying altered colony morphology. A gene cluster was identified that appears to encode a completewzy/wzx-dependent polysaccharide assembly pathway. Deleting individual genes in this cluster caused a non-mucoid phenotype and a corresponding loss of EPS secretion, confirming that LM7 assembles a novelwzy/wzx-dependent polysaccharide. We extracted EPS from LM7 cultures and showed that it contains a linear chain of 3- and 4-linked glucose, galactose, and glucuronic acid residues. Finally, we found that the EPS pathway we identified diverges from those of adhesive polysaccharides such as the holdfast that are conserved in higherAlphaproteobacteria.Our approach of characterizing complete biosynthetic pathways holds promise for engineering of polysaccharides with valuable properties.ImportanceBacteria produce complex polysaccharides that serve a range of biological functions. These polymers often have properties that make them attractive for industrial applications, but they remain woefully underutilized. In this work, we studied a novel polysaccharide called promonan that is produced bySphingomonas sp.LM7, a bacterium we isolated from Lake Michigan. We extracted promonan from LM7 cultures and identified which sugars are present in the polymer. We also identified the genes responsible for polysaccharide production. Comparing the promonan genes to those of other bacteria showed that promonan is distinct from previously characterized polysaccharides. We conclude by discussing how the promonan pathway could be used to produce new polysaccharides through genetic engineering.

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mentioning

confidence: 99%

A novel exopolysaccharide pathway from a freshwaterSphingomonasisolate

Goetsch,

Ufearo,

Keiser

et al. 2023

Preprint

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Fed‐batch fermentation strategy for efficient welan gum production by Sphingomonas sp. FM01

Liu,

He,

Wei

et al. 2024

J Sci Food Agric

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BACKGROUNDAs a novel type of extracellular polysaccharide produced by Sphingomonas sp., welan gum has been widely applied in various fields because of its excellent properties. The study has improved the fermentation process.ResultsThe initial sucrose concentration, temperature and stirring speed were set to 20 g L−1, 33 °C and 400 rpm, respectively, and 13.3 g L−1 sucrose was added at 24, 40 and 56 h. The temperature and stirring speed were then set at 28 °C and 600 rpm from 24 to 48 h and 28 °C and 600 rpm from 48 to 72 h, respectively. As a result, welan gum production, dry cell weight, sucrose conversion rate and viscosity were correspondingly increased to 38.60 g L−1, 5.47 g L−1, 0.64 g g−1 and 3779 mPa·s, respectively. In addition, the mechanism by which fermentation strategy promotes welan gum synthesis was investigated by transcriptome analysis.CONCLUSIONImproving respiration and ATP supply, reducing unnecessary protein synthesis, and alleviating competition between cell growth and welan gum synthesis contribute to promoting the fermentation performance of Sphingomonas sp., thus providing a practical strategy for efficient welan gum production. © 2024 Society of Chemical Industry.

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