High-quality urban green space supports the healthy functioning of urban ecosystems. This study aimed to rapidly assess the distribution, and accurately estimate the above-ground biomass, of urban green space using remote sensing methods, thus providing a better understanding of the urban ecological environment in Xuzhou for more effective management. We performed urban green space classifications and compared the performance of Sentinel-2 MSI data and Sentinel-1 SAR data and combinations, for estimating above-ground biomass, using field data from Xuzhou, China. The results showed the following: (1) incorporating an object-oriented method and random forest algorithm to extract urban green space information was effective; (2) compared with stepwise regression models with single-source data, biomass estimation models based on multi-source data provide higher estimation accuracy (R2 = 0.77 for coniferous forest, R2 = 0.76 for shrub-grass vegetation, R2 = 0.75 for broadleaf forest); and (3) from 2016 to 2021, urban green space coverage in Xuzhou decreased, while the total above-ground biomass increased, with higher average above-ground biomass in broadleaf forests (133.71 tons/ha) compared to coniferous forests (92.13 tons/ha) and shrub-grass vegetation (21.65 tons/ha). Our study provides an example of automated classification and above-ground biomass mapping for urban green space using multi-source data and facilitates urban eco-management.