Cotton fiber germin-like protein. I. Molecular cloning and gene expression

Kim, Hee Jin; Triplett, Barbara A.

doi:10.1007/s00425-003-1133-1

Cited by 60 publications

(41 citation statements)

References 66 publications

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“…1). This pattern of protein accumulation is consistent with the expression pattern of the GhGLP1 transcripts (Kim and Triplett 2003). An abundant protein of 25.5 kDa was evident in 12 DPA developing cotton fibers (Fig.…”

Section: Resultssupporting

confidence: 83%

“…The gene for GhGLP1 is specifically expressed in cotton fiber and is developmentally regulated (Kim and Triplett 2003). To investigate the pattern of GhGLP1 protein accumulation during fiber development, two antibodies were produced.…”

Section: Resultsmentioning

confidence: 99%

“…Our differential display screen identified a cotton fiber GLP (GhGLP1) whose expression predominated during the cell wall expansion phase of fiber development (Kim and Triplett 2003). In this report we have purified GhGLP1 from developing cotton fibers and tested whether any of the enzymatic activities previously linked with GLPs are associated with the cotton fiber protein.…”

Section: Introductionmentioning

confidence: 98%

“…In an effort to clone developmentally regulated genes that are fiber-specific in their expression patterns, a differential display screen (Liang and Pardee 1991) was conducted, comparing wild type cotton, TM1 (Kohel et al 1970), and a near-isogenic naked seed mutant, N1 (Kim and Triplett 2003). Nucleotide analysis of one of the differential display amplification products showed sequence similarity to members of the cupin gene superfamily (Kim and Triplett 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Nucleotide analysis of one of the differential display amplification products showed sequence similarity to members of the cupin gene superfamily (Kim and Triplett 2003). Cupins are characterized by their conserved b-barrel structure but are functionally diverse proteins (Dunwell 1998;Dunwell et al 2001;Khuri et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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Cotton-fiber germin-like protein. II: Immunolocalization, purification, and functional analysis

Kim

Pesacreta

Triplett

2004

Planta

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Cotton (Gossypium hirsutum L.) contains a germin-like protein (GLP), GhGLP1, that shows tissue-specific accumulation in fiber. The fiber GLP is an oligomeric, glycosylated protein with a subunit size of approximately 25.5 kDa. Accumulation of GhGLP1 occurs during the period of fiber elongation [4-14 days post-anthesis (DPA)]. During early phases of fiber development (2-4 DPA), GhGLP1 localizes to cytoplasmic vesicles as shown by confocal immunofluorescent microscopy. In slightly older fibers (7-10 DPA), GhGLP1 localizes to the apoplast. In other plants, germins and GLPs have been reported to have enzymatic activities including oxalate oxidase (OxO), superoxide dismutase, and ADP-glucose pyrophosphatase. Cotton fiber extracts did not contain OxO activity, nor did intact fibers stain for OxO activity. A four-step purification protocol involving ammonium sulfate precipitation of a 1.0 M NaCl extract, ion-exchange chromatography on DEAE-Trisacryl M, lectin-affinity chromatography, and gel filtration chromatography resulted in electrophoretically pure GhGLP1. While 1.0 M NaCl extracts from 10-14 DPA fiber contained superoxide dismutase and phosphodiesterase activities, GhGLP1 could be separated from both enzyme activities by the purification protocol. Although a GLP accumulates in the cotton fiber apoplast during cell elongation, the function of this protein in fiber growth and development remains unknown.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cotton-fiber germin-like protein. II: Immunolocalization, purification, and functional analysis

Kim

Pesacreta

Triplett

2004

Planta

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Cotton

Rathore

Sunilkumar

Cantrell

et al. 2008

Compendium of Transgenic Crop Plants

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Lint obtained from cotton plant represents the most important natural fiber that has been used for over seven millennia to meet the clothing requirement of mankind. In addition to the fiber, the plant also provides seed, linters, and hull that are used for food, feed, and several other diverse applications. The cotton plant is susceptible to a large number of pests and pathogens. Traditionally, its cultivation has relied more heavily on the use of toxic pesticides compared to many other crops. Due to the challenges posed by biotic and abiotic stresses during its cultivation and because of the large number of useful products derived from this plant, cotton offers numerous targets that can be modified through the use of modern biotechnological tools. The first generation of genetically engineered cotton plants, offering insect‐ and herbicide‐resistance traits, have gained rapid and wide acceptance in many cotton‐growing countries around the globe. The chapter provides a comprehensive account of transgenic cotton production, including transformation methods, traits introduced, intellectual property rights, and the regulatory issues involved. A brief overview of history, origin, distribution, taxonomy, economic importance, breeding strategies, and achievements is also presented for this crop.

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The proteome of maritime pine wood forming tissue

et al. 2005

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Wood is one of our most important natural resources. Surprisingly, we know hardly anything about the details of the process of wood formation. The aim of this work was to describe the main proteins expressed in wood forming tissue of a conifer species (Pinus pinaster Ait.). Using high resolution 2-DE with linear pH gradient ranging from 4 to 7, a total of 1039 spots were detected. Out of the 240 spots analyzed by MS/MS, 67.9% were identified, 16.7% presented no homology in the databases, and 15.4% corresponded to protein mixtures. Out of the 57 spots analyzed by MALDI-MS, only 15.8% were identified. Most of the 175 identified proteins play a role in either defense (19.4%), carbohydrates (16.6%) and amino acid (14.9%) metabolisms, genes and proteins expression (13.1%), cytoskeleton (8%), cell wall biosynthesis (5.7%), secondary (5.1%) and primary (4%) metabolisms. A summary of the identified proteins, their putative functions, and behavior in different types of wood are presented. This information was introduced into the PROTICdb database and is accessible at http://cbib1.cbib.u-bordeaux2.fr/Protic/Protic/home/index.php. Finally, the average protein amount was compared with their respective transcript abundance as quantified through EST counting in a cDNA-library constructed with mRNA extracted from wood forming tissue.

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Cotton fiber germin-like protein. I. Molecular cloning and gene expression

Cited by 60 publications

References 66 publications

Cotton-fiber germin-like protein. II: Immunolocalization, purification, and functional analysis

Cotton-fiber germin-like protein. II: Immunolocalization, purification, and functional analysis

Cotton

The proteome of maritime pine wood forming tissue

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