Cotton microtubule-associated protein GhMAP20L5 mediates fiber elongation through the interaction with the tubulin GhTUB13

Song, Qingwei; Gao, Wanting; Du, Changming; Wang, Jin; Zuo, Kaijing

doi:10.1016/j.plantsci.2022.111545

Cited by 12 publications

(7 citation statements)

References 53 publications

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“…Several MAPs and microtubule proteins have been reported to be involved in cotton fiber development, such as GhMAP20L5, GhTUB1 and GhTUA9 [43][44][45]. However, the specific functions of MAPs and microtubules in cotton plant height regulation remain unclear.…”

Section: Discussionmentioning

confidence: 99%

Repression of GhTUBB1 Reduces Plant Height in Gossypium hirsutum

Zhang,

Ma,

Wang

et al. 2023

IJMS

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The original ‘Green Revolution’ genes are associated with gibberellin deficiency. However, in some species, mutations in these genes cause pleiotropic phenotypes, preventing their application in dwarf breeding. The development of novel genotypes with reduced plant height will resolve this problem. In a previous study, we obtained two dwarf lines, L28 and L30, by introducing the Ammopiptanthus mongolicus (Maxim. ex Kom.) Cheng f. C-repeat-binding factor 1 (AmCBF1) into the upland cotton variety R15. We found that Gossypium hirsutum Tubulin beta-1 (GhTUBB1) was downregulated in L28 and L30, which suggested that this gene may have contributed to the dwarf phenotype of L28 and L30. Here, we tested this hypothesis by silencing GhTUBB1 expression in R15 and found that decreased expression resulted in a dwarf phenotype. Interestingly, we found that repressing AmCBF1 expression in L28 and L30 partly recovered the expression of GhTUBB1. Thus, AmCBF1 expression presented a negative relationship with GhTUBB1 expression in L28 and L30. Moreover, yeast one-hybrid and dual-luciferase assays suggest that AmCBF1 negatively regulates GhTUBB1 expression by directly binding to C-repeat/dehydration-responsive (CRT/DRE) elements in the GhTUBB1 promoter, potentially explaining the dwarf phenotypes of L28 and L30. This study elucidates the regulation of GhTUBB1 expression by AmCBF1 and suggests that GhTUBB1 may be a new target gene for breeding dwarf and compact cultivars.

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Section: Discussionmentioning

confidence: 99%

Repression of GhTUBB1 Reduces Plant Height in Gossypium hirsutum

Zhang,

Ma,

Wang

et al. 2023

IJMS

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“…Recently, cotton microtubule-associated protein GhMAP20L5 was reported to mediate fiber elongation through the interaction with the tubulin GhTUB13. In the RNA silencing plants, GhMAP20L5 expression was repressed at least 28.1% in comparison with its null plants at different development stages (0 DPA, 6 DPA, 12 DPA, and 18 DPA) which resulted in reducing fiber elongation rate, fiber length and lint percentage [35].…”

Section: Fiberless Mutants Identificationmentioning

confidence: 95%

Genetic and Molecular Regulation of Cotton Fiber Initiation and Elongation

Bai,

Scheffler

2024

Preprint

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Cotton fiber, a crucial and sustainable resource for global textile production, undergoes a complex five-stage developmental process, encompassing initiation, elongation, transition, secondary cell wall biosynthesis, and maturation. These elongated single-cell fibers originate from the outer ovule epidermis. The development of cotton fibers involves intricate changes in gene expression and physiological processes, resulting in a nearly pure cellulose product vital for the global cotton industry. Decoding genes associated with fiber development enhances our understanding of cotton fiber mechanisms and facilitates the cultivation of varieties with enhanced quality. In recent decades, advanced omics approaches, including genomics, transcriptomics, and proteomics, have played a pivotal role in identifying genes and gene products linked to cotton fiber development, including the MYB transcription factor family which coordinates cotton fiber development. Molecular studies have revealed transcription factors like MYB, WRKY, Homeodomain Leucine Zipper (HD-ZIP) and basic Helix-Loop-Helix (bHLH) influencing fiber initiation and elongation. The intricate interplay of phytohormones like auxin, gibberellic acid (GA), brassinosteroid (BR), jasmonic acid (JA), ethylene, abscisic acid (ABA), and cytokinin is explored, providing a comprehensive perspective on shaping cotton fibers. Numerous candidate genes and cellular processes affecting various aspects of fiber development hold promise for genetic engineering or marker-assisted breeding to improve fiber quality. This review presents a comprehensive overview of key achievements in cotton molecular biology, with a specific emphasis on recent advancements in understanding transcription factors and phytohormones involved in cotton fiber initiation and elongation.

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“…TRM4 and IQD9 maintain cortical MT organization to orchestrate cellulose patterning in Arabidopsis seed mucilage [ 152 , 153 ]. MTs are important for the development and diameter determination of cotton fiber [ 154 ], an extension of seed coat [ 155 ], as shown by a recent finding that GhMAP20L5 is involved in cotton fiber elongation by interacting with GhTUB13 [ 156 ].…”

Section: Mts In Morphological Developmentmentioning

confidence: 99%

Microtubule Regulation in Plants: From Morphological Development to Stress Adaptation

Hsiao

Huang

2023

Biomolecules

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Microtubules (MTs) are essential elements of the eukaryotic cytoskeleton and are critical for various cell functions. During cell division, plant MTs form highly ordered structures, and cortical MTs guide the cell wall cellulose patterns and thus control cell size and shape. Both are important for morphological development and for adjusting plant growth and plasticity under environmental challenges for stress adaptation. Various MT regulators control the dynamics and organization of MTs in diverse cellular processes and response to developmental and environmental cues. This article summarizes the recent progress in plant MT studies from morphological development to stress responses, discusses the latest techniques applied, and encourages more research into plant MT regulation.

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Cotton microtubule-associated protein GhMAP20L5 mediates fiber elongation through the interaction with the tubulin GhTUB13

Cited by 12 publications

References 53 publications

Repression of GhTUBB1 Reduces Plant Height in Gossypium hirsutum

Repression of GhTUBB1 Reduces Plant Height in Gossypium hirsutum

Genetic and Molecular Regulation of Cotton Fiber Initiation and Elongation

Microtubule Regulation in Plants: From Morphological Development to Stress Adaptation

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