Cotton (Gossypium hirsutum L.) is responsive to boron (B) fertilization when there is low soil availability, but the best source and rate to be used and whether this response is dependent on soil texture are still unknown. This study aimed to adjust boron fertilization for cotton as a function of the production environment and B source used. Two field experiments were conducted in the 2020/2021 season in Chapadão do Sul, MS (clayey soil—adequate B content) and Dracena, SP (sandy soil—low B content), Brazil. Treatments consisted of B sources (ulexite [low solubility], borax pentahydrate [BP] [intermediate solubility], and boric acid [BA] [high solubility]), and B rates (0, 1, 2, 4, and 6 kg ha−1) applied to the soil at 25 days after plant emergence. In sandy soil with low B content, application of 2 (high and medium solubility sources) and 4 kg B ha−1 (low solubility source) improved fiber yield between 10% (210 kg ha−1 fiber) and 28% (555 kg ha−1), respectively, as well as micronaire index, strength, elongation, uniformity, and short fibers. Application of B greater than 4 kg ha−1 via soluble sources reduced (between 9% (175 kg ha−1)—BP and 14% (257 kg ha−1)—BA) fiber yield only in sandy soil. When B content in the soil is adequate, B fertilization did not improve yield, but increased fiber strength (4%—1.1 g tex−1) and reduced the short fiber index (16%) by applying 1 kg B ha−1, regardless of the source used. The highest fiber yields were obtained with leaf B contents between 12 and 17 mg kg−1 (sandy soil) and 25 and 27 mg kg−1 (clayey soil). We recommend applying 2 kg B ha−1 (solubility sources) and 4 kg B ha−1 (low solubility source) in sandy soils with low B content to improve yield and fiber quality, and 1 kg B ha−1 in clayey soil with adequate B content to improve fiber quality and replace B amounts removed through harvesting.