Could antioxidant capacity and flavonoid content of ethanolic extracts of geopropolis from Brazilian native bees be estimated from digital photos and NIR Spectra?

Turco, João Fábio; Nascimento, Camila L. do; Lima, Vanderlei Aparecido de; Torres, Yohandra Reyes

doi:10.1016/j.microc.2020.105031

Cited by 14 publications

(5 citation statements)

References 56 publications

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“…The onion peel extracts were prepared according to [ 10 ] with slight modifications. The peel was detached from the onion and crushed in small pieces.…”

Section: Methodsmentioning

confidence: 99%

“…A large number of analytical methods have been proposed for bioflavonoid detection in different samples. These methods include molecular spectroscopy [ 7 , 8 , 9 , 10 , 11 ], high-performance liquid chromatography [ 12 , 13 , 14 , 15 , 16 , 17 ], capillary electrophoresis [ 18 , 19 ], and different electrochemical methods, mainly voltammetry [ 20 , 21 , 22 ]. Generally, molecular spectroscopy and electrochemical methods can be used for fast, simple, and nonselective assessment of the content of flavonoids in samples, while chromatographic approaches allow the determination of individual bioflavonoids with a sensitivity that is several orders of magnitude higher than the sensitivity of molecular spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

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Fast and Sensitive Determination of Bioflavonoids Using a New Analytical System Based on Label-Free Silver Triangular Nanoplates

Furletov

Apyari

Гаршев

et al. 2022

Sensors

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Optical sensors based on silver triangular nanoplates (AgTNPs) are insufficiently studied as probes for the spectrophotometric determination of biologically active compounds. In the present article, an interaction between label-free AgTNPs and bioflavonoids in the presence of silver(I) ions was assessed to outline the possibilities of AgTNPs as a colorimetric probe for the fast and sensitive determination of bioflavonoids. It is shown that the interaction was accompanied by a bathochromic shift of the local surface plasmon resonance band of nanoparticles and an increase in its intensity. Seven bioflavonoids differing in their structure were tested. The influence of the structure of analytes and the main external factors on the analytical signal is discussed in detail. It was found that the detection limits of bioflavonoids in the selected optimal conditions increased in the series morin < rutin = quercetin < taxifolin and came to 0.9, 1.2, 1.2, and 2.0 μmol·L−1, respectively. Chrysin, naringenin, and naringin were found not to affect the spectral characteristics of AgTNPs. The suggested approach was applied for the spectrophotometric determination of flavonoids in pharmaceuticals and onion peel.

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“…The onion peel extracts were prepared according to [ 10 ] with slight modifications. The peel was detached from the onion and crushed in small pieces.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fast and Sensitive Determination of Bioflavonoids Using a New Analytical System Based on Label-Free Silver Triangular Nanoplates

Furletov

Apyari

Гаршев

et al. 2022

Sensors

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“…The variability of antibacterial properties among the different extracts of the genus Melipona and the species of S. depilis is a reflection of several factors, such as the difference in chemical composition and concentration of active compounds, which can be different due to the characteristics of each species or population or also due to differences in plant biodiversity visited by bees during resin collection, the time of year, and the different solvents used in the material extraction process. [52,53] Growth inhibition by extracts may be associated with the complex chemical composition of geopropolis and the polarity of the solvent used in the extraction process. Ethyl acetate is constantly used due to its high selectivity in the extraction of phenolic, polyphenolic, flavonoid compounds and other bioactive molecules that can favour the process of bacterial inhibition.…”

Section: Geopropolis Extracts Have Been Investigated For Presenting C...mentioning

confidence: 99%

Antibacterial and Toxic Activity of Geopropolis Extracts from Melipona subnitida (Ducke, 1910) (Hymenoptera: Apidae) and Scaptotrigona depilis (Moure, 1942) (Hymenoptera: Apidae)

Costa dos Santos,

Silva Macêdo,

de Sousa Silveira

et al. 2023

Chemistry & Biodiversity

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Bacteria are associated with many infections that affect humans and present antibiotic resistance mechanisms, causing problems in health organisations and increased mortality rates. Therefore, it is necessary to find new antibacterial agents that can act in the treatment of these microorganisms. Geopropolis is a natural product made by stingless bees, formed by a mixture of plant resins, salivary secretions, wax and soil particles, presenting a diverse chemical composition. Thus, this study aimed to evaluate antibacterial activity, antibiotic modulation and the toxicity of geopropolis extract from the stingless bees, Melipona subnitida (Ducke, 1910) and Scaptotrigona depilis (Moure, 1942) against standard and multi‐resistant Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa bacteria. Geopropolis samples were collected in a meliponary located in Camaragibe, Pernambuco, Brazil. To determine the Minimum Inhibitory Concentration (MIC) and antibiotic modulation we performed broth microdilution tests. Mortality tests were used to verify extract toxicity in the model Drosophila melanogaster. The microbiological tests showed that the M. subnitida extracts had better inhibitory effects compared to S. depilis, presenting direct antibacterial activity against standard and multi‐resistant strains. The extracts potentialized antibiotic effects, suggesting possible synergy and did not present toxicity in the model used.

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“…Some studies have demonstrated that the presence of compounds such as flavonoids, aromatic acids and their esters, terpenoids and carotenoids, among others [18][19][20], is related to biological properties such as the antioxidant, antimicrobial and antileishmanial activities found in propolis [21][22][23][24]. In this sense, American Cutaneous Leishmaniasis (ACL) is a polymorphic, spectral disease of the skin and mucous membranes, which is divided into several clinical forms, such as the cutaneous, mucosal cutaneous and diffuse cutaneous ones [24].…”

Section: Introductionmentioning

confidence: 99%

Preliminary Study on the Chemical and Biological Properties of Propolis Extract from Stingless Bees from the Northern Region of Brazil

Ferreira,

Souza,

Pereira

et al. 2024

Processes

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Natural products are historically regarded as the main sources of bioactive compounds to fight the most diverse diseases; among them, propolis deserves to be highlighted due to several biological activities, such as antioxidant, anti-inflammatory, healing, antibacterial and antileishmanial activity. In this study, the antioxidant and antipromastigote activities and the cytotoxicity of propolis of the Scaptotrigona aff. postica stingless bee were evaluated. Propolis extract was analyzed by infrared spectroscopy, thermogravimetry and spectrophotometry for total polyphenols and flavonoid contents. Antioxidant activity was assessed spectrophotometrically by the DPPH, ABTS and FRAP methods, while antipromastigote activity and cytotoxicity were assessed by the MTT assay. The infrared spectra showed the characteristic bands of both phenols and alcohols. The thermogravimetry study revealed two events and thermal stability around 370 °C, while differential scanning calorimetry showed two endothermic events and an exothermic event. Total polyphenol and flavonoid contents were 21.29 ± 0.003 mg/g and 8.17 ± 0.00 mg/g, respectively, and the antioxidant activities identified by the DPPH, ABTS and FRAP methods were 910.09 ± 0.0264 µM Trolox (IC50 18.9 ± 0.01 µg/mL), 859.97 ± 0.0854 µM Trolox (IC50 692 ± 0.01 µg/mL) and 1613.67 ± 0.2610 µM Trolox (IC50 80 ± 0.1 µg/mL), respectively. The satisfactory antioxidant activity of propolis can be related to the high content of phenolic compounds, which adds value to this product and can contribute to the development of meliponiculture in the Brazilian state of Pará. The propolis extract caused a toxic effect on promastigote forms of Leishmania amazonensis at all concentrations tested, with an IC50 value of 1.50 µg/mL and a statistically significant difference compared to the negative control (p < 0.001). These results show that the propolis extract from S. postica bees may be a promising alternative for treatment against promastigote forms of L. amazonensis.

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Could antioxidant capacity and flavonoid content of ethanolic extracts of geopropolis from Brazilian native bees be estimated from digital photos and NIR Spectra?

Cited by 14 publications

References 56 publications

Fast and Sensitive Determination of Bioflavonoids Using a New Analytical System Based on Label-Free Silver Triangular Nanoplates

Fast and Sensitive Determination of Bioflavonoids Using a New Analytical System Based on Label-Free Silver Triangular Nanoplates

Antibacterial and Toxic Activity of Geopropolis Extracts from Melipona subnitida (Ducke, 1910) (Hymenoptera: Apidae) and Scaptotrigona depilis (Moure, 1942) (Hymenoptera: Apidae)

Preliminary Study on the Chemical and Biological Properties of Propolis Extract from Stingless Bees from the Northern Region of Brazil

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