Could <i>meta-</i>Topoline improve the multiplication and rooting of micropropagated walnut plants? – a case study with ‘Lara’ (<i>Juglans regia</i> L.)

Nacheva, L.; Gandev, S.

doi:10.17660/actahortic.2023.1359.10

Acta Hortic.

2023

DOI: 10.17660/actahortic.2023.1359.10

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Could meta-Topoline improve the multiplication and rooting of micropropagated walnut plants? – a case study with ‘Lara’ (Juglans regia L.)

L. Nacheva¹,

S. Gandev²

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2024

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Cited by 2 publications

References 32 publications

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Effects of meta-topolin riboside and meta-methoxy topolin riboside on the in vitro micropropagation of Pyrus communis L.

Dimitrova,

Nacheva,

Aleksandrova

et al. 2024

Acta Sci. Pol. Hortorum Cultus

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The present study aimed to evaluate the effects of new meta-topolin derivatives meta-topolin riboside (mTR) and meta-methoxy topolin riboside (memTR) on the ultiplication and subsequent rooting and ex vitro acclimatization of Pyrus communis L. (‘OHF 333’). The cytokinins mTR and memTR were included in the nutrient medium (0 μM, 3 μM, 6 μM, 9 μM, 12 μM). In plants from three passages of three-week-old cultures grown on different nutrient media, the following parameters were evaluated: multiplication coefficient, fresh (FW) and dry (DW) weight (mg), average length of shoots (mm), average number of leaves, leaf length and width (mm). At the rooting stage, data on the rooting frequency, number of roots per rooted micro-cutting and the length of roots were recorded 18 days after the start of the experiment. In the acclimatized plants, leaf area, FW and DW, and the content of photosynthetic pigments were determined 40 days after the transfer to ex vitro conditions. Gas exchange rate and chlorophyll fluorescence were also evaluated for the control and the variants with 6 and 9 μM mTR and memTR. The plantlets grown on cytokinin-supplemented media showed a higher number of leaves than the control. Plantlets grown on nutrient media with 6 and 12 μM mTR were distinguished by the highest FW and DW. In these variants, the shoots were of the greatest length. The plants grown on medium with 6 μM mTR had the highest number of leaves. Control plants had larger leaves. Тhe highest rooting percentage (70%) was achieved in plantlets grown with 9 μM mTR. A higher ex vitro acclimatization survival rate (76–100%) was found in all plants cultured with mTR or memTR compared to control plants (65%).

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Effects of meta-topolin riboside and meta-methoxy topolin riboside on the in vitro micropropagation of Pyrus communis L.

Dimitrova,

Nacheva,

Aleksandrova

et al. 2024

Acta Sci. Pol. Hortorum Cultus

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Assessment of Micropropagation Possibilities for Japanese Hascap (Lonicera caerulea var. emphyllocalyx L.)

Basara,

Litwińczuk,

Gorzelany

2024

Applied Sciences

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In recent years, interest in Lonicera caerulea production has grown significantly because of its nutritional and pharmaceutical benefits, leading to rapid expansion in its cultivation. L caerulea var. emphyllocalyx is a lesser-known botanical variety. Due to differences between plants of the Lonicera genus and the lack of scientific reports on micropropagation, it is necessary to determine the possibilities of in vitro propagation. The aim of this study was to elaborate a micropropagation protocol of two new breeding clones of Lonicera caerulea var. emphyllocalyx: ‘21–17’ and ‘139–24’. The experiments were carried out on in vitro cultures grown on MS medium supplemented with 1 mg·dm−3 BA or 1 mg·dm−3 mT. Two types of explants were used during the experiment: nodal fragments (NFs) and shoot-tips (STs). Before acclimatisation, some rooted microshoots were subjected to cooling at 4 °C for 4 weeks. Significantly more ST explants than NF explants started to grow at the proliferation stage. The application of BA resulted in much better proliferation and health of cultures. Cold storage of micropropagated ‘139–24’ plantlets significantly increased their survival in acclimatisation in contrast to ‘21–17’ plantlets but weakened further growth of the plants. In future in vitro studies on L. caerulea var. emphyllocalyx, BA can be used as the primary growth regulator due to its effectiveness and low cost. Nodal fragments should be considered as the main propagation material since they promote better growth rates. Additionally, further research is required to explore the effects of low-temperature storage on the growth and physiology of these plants. The results obtained in this research may contribute to the development of micropropagation technology in the future for L. caerulea var. emphyllocalyx.

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Could meta-Topoline improve the multiplication and rooting of micropropagated walnut plants? – a case study with ‘Lara’ (Juglans regia L.)

Cited by 2 publications

References 32 publications

Effects of meta-topolin riboside and meta-methoxy topolin riboside on the in vitro micropropagation of Pyrus communis L.

Effects of meta-topolin riboside and meta-methoxy topolin riboside on the in vitro micropropagation of Pyrus communis L.

Assessment of Micropropagation Possibilities for Japanese Hascap (Lonicera caerulea var. emphyllocalyx L.)

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