Coumarin Communication Along the Microbiome–Root–Shoot Axis

Stassen, Max J. J.; Hsu, Shu-Hua; Pieterse, Corné M. J.; Stringlis, Ioannis A.

doi:10.1016/j.tplants.2020.09.008

Cited by 136 publications

(81 citation statements)

References 112 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reminiscent to the bidirectional communication mechanisms described along the microbiota-gut-brain axis in animals ( Carabotti et al, 2015; Dinan and Cryan 2017, Stassen et al, 2020 ), we report here that aboveground stress responses in plants can be orchestrated through long-distance communication with belowground commensals. We demonstrate that change in aboveground light can cascade along the shootroot axis to alter the composition of root commensal communities.…”

Section: Discussionsupporting

confidence: 62%

Microbiota-root-shoot axis modulation by MYC2 favoursArabidopsisgrowth over defence under suboptimal light

Hou

Thiergart

Vannier

et al. 2020

Preprint

View full text Add to dashboard Cite

Bidirectional root-shoot signalling is likely key in orchestrating stress responses and ensuring plant survival. Here we show that Arabidopsis thaliana responses to microbial root commensals and light are interconnected along a microbiota-root-shoot axis. Microbiota and light manipulation experiments in a gnotobiotic system reveal that low photosynthetically active radiation (LP) perceived by leaves induce longdistance modulation of root bacterial, but not fungal or oomycetal communities. Reciprocally, bacterial root commensals and particularly Pseudomomas isolates are necessary for rescuing plant growth under LP. RNA-Seq, combined with leaf inoculation experiments with biotrophic and necrotrophic microbial pathogens indicate that microbiota-induced growth under LP coincides with transcriptional repression of immune responses, thereby increasing susceptibility to both pathogens. Inspection of a set of A. thaliana mutants demonstrates that orchestration of this light-dependent growth-defence trade-off requires the transcriptional regulator MYC2. Our work indicates that aboveground stress responses in plants can be governed by signals from microbial root commensals.

show abstract

Section: Discussionsupporting

confidence: 62%

Microbiota-root-shoot axis modulation by MYC2 favoursArabidopsisgrowth over defence under suboptimal light

Hou

Thiergart

Vannier

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…This was further confirmed by evidence showing that coumarins could shape the composition of a SynCom, where the abundance of Pseudomonas was significantly higher in coumarin-deficient Arabidopsis f6'h1 mutants than in wild-type plants (Voges et al, 2019). A recent excellent review described coumarins as the "new kids on the block" in the chemical communications between plant roots and root microbiomes (Stassen et al, 2020).…”

Section: Coumarin Benzoxazinoid Terpenes and Other Root-exuded Molmentioning

confidence: 83%

Linking Plant Secondary Metabolites and Plant Microbiomes: A Review

et al. 2021

View full text Add to dashboard Cite

Plant secondary metabolites (PSMs) play many roles including defense against pathogens, pests, and herbivores; response to environmental stresses, and mediating organismal interactions. Similarly, plant microbiomes participate in many of the above-mentioned processes directly or indirectly by regulating plant metabolism. Studies have shown that plants can influence their microbiome by secreting various metabolites and, in turn, the microbiome may also impact the metabolome of the host plant. However, not much is known about the communications between the interacting partners to impact their phenotypic changes. In this article, we review the patterns and potential underlying mechanisms of interactions between PSMs and plant microbiomes. We describe the recent developments in analytical approaches and methods in this field. The applications of these new methods and approaches have increased our understanding of the relationships between PSMs and plant microbiomes. Though the current studies have primarily focused on model organisms, the methods and results obtained so far should help future studies of agriculturally important plants and facilitate the development of methods to manipulate PSMs–microbiome interactions with predictive outcomes for sustainable crop productions.

show abstract

“…Because overexpression of BGLU42 confers resistance against diverse plant pathogens, it is tempting to speculate that MYB72-dependent BGLU42 activity mobilizes a coumarin that then functions as a systemically transported ISR signal to prime systemic tissues for enhanced defense ( Fig. 3; Stassen et al 2020). However, this hypothesis remains to be tested.…”

Section: The Root Of Wcs417-isrmentioning

confidence: 99%

“…Besides their function in the mobilization and uptake of iron from the soil environment, coumarins such as scopoletin, possess a selective antimicrobial activity (Stassen et al 2020;Stringlis et al 2019a). Using the mutant f6'h1, which is impaired in the coumarin biosynthesis gene FERULOYL-CoA 6'HYDROXYLASE1, it was shown that F6'H1-dependent coumarins have a significant effect on the composition of microbiota in the rhizosphere or in gnotobiotic conditions using microbial synthetic communities (Harbort et al 2020;Stringlis et al 2018b;Voges et al 2019).…”

Section: Among the Stars: Wcs417 In A Root Microbiome Contextmentioning

confidence: 99%

Pseudomonas simiae WCS417: star track of a model beneficial rhizobacterium

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background Since the 1980s, numerous mutualistic Pseudomonas spp. strains have been used in studies on the biology of plant growth-promoting rhizobacteria (PGPR) and their interactions with host plants. In 1988, a strain from the Pseudomonas fluorescens group, WCS417, was isolated from lesions of wheat roots growing in a take-all disease-suppressive soil. In subsequent trials, WCS417 limited the build-up of take-all disease in field-grown wheat and significantly increased wheat yield. In 1991, WCS417 was featured in one of the first landmark studies on rhizobacteria-induced systemic resistance (ISR), in which it was shown to confer systemic immunity in carnation (Dianthus caryophyllus) against Fusarium wilt. The discovery that WCS417 conferred systemic immunity in the model plant species Arabidopsis thaliana in 1996 incited intensive research on the molecular mechanisms by which PGPR promote plant growth and induce broad-spectrum disease resistance in plants. Since then, the strain name appeared in over 750 studies on beneficial plant-microbe interactions. Scope In this review, we will highlight key discoveries in plant-microbe interactions research that have emerged from over 30 years of research featuring WCS417 as a model rhizobacterial strain. WCS417 was instrumental in improving our understanding of the microbial determinants that are involved in root colonization and the establishment of mutually beneficial interactions with the host plant. The model strain also provided novel insight into the molecular mechanisms of plant growth promotion and the onset and expression of rhizobacteria-ISR. More recently, WCS417 has been featured in studies on host immune evasion during root colonization, and chemical communication in the rhizosphere during root microbiome assembly. Conclusions Numerous studies on the modes of action of WCS417 have provided major conceptual advances in our understanding of how free-living mutualists colonize the rhizosphere, modulate plant immunity, and promote plant growth. The concepts may prove useful in our understanding of the molecular mechanisms involved in other binary plant-beneficial microbe interactions, and in more complex microbial community contexts, such as the root microbiome.

show abstract

Coumarin Communication Along the Microbiome–Root–Shoot Axis

Cited by 136 publications

References 112 publications

Microbiota-root-shoot axis modulation by MYC2 favoursArabidopsisgrowth over defence under suboptimal light

Microbiota-root-shoot axis modulation by MYC2 favoursArabidopsisgrowth over defence under suboptimal light

Linking Plant Secondary Metabolites and Plant Microbiomes: A Review

Pseudomonas simiae WCS417: star track of a model beneficial rhizobacterium

Contact Info

Product

Resources

About