Coumarin derivatives enhance the chemiluminescence accompanying lipid peroxidation

Vladimirov, Yu. A.; Sharov, Victor S.; Driomina, Elena S.; Reznitchenko, Alexander V.; Gashev, Sergey B.

doi:10.1016/0891-5849(94)00199-t

Cited by 35 publications

(18 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is suggested to apply an activator that enters into such interactions either extremely limited or does not enter at all. As reported by [21,22,23], isoquinolysin derivatives of coumarin are being the similar activators, besides being as well lipoperoxidase reactionsspecific. Coumarin-334 was applied in this study as a chemiluminescence activator.…”

supporting

confidence: 68%

Cytochromecas a facultative enzyme – lipoperoxidase and it’s inhibition by antioxidants

Romodin

Vladimirov

Lysenko

et al. 2019

Preprint

View full text Add to dashboard Cite

Many researchers consider a key role in initiation of apoptosis along the mitochondrial pathway to be enhanced by cytochrome c, one of the components of the mitochondrial respiratory chain, which acquires peroxidase activity by forming a complex with phospholipids. Mitochondrial membranes are destroyed affected by the peroxidase reaction catalyzed by this supramolecular nanoparticle, resulting in the release of various proapoptotic factors into the cellular cytoplasm, ultimately leading to the development of an apoptosis pathway. The study of lipoperoxidase activity of the cytochrome c with cardiolipin complex is conducted via activated chemiluminescence. However, prior to this study, no assessment of the potential contribution of free non-heme iron, which can be inserted into the sample, into chemiluminescence of the system of cytochrome c complex with cardiolipinhydrogen peroxide. It was found during the study process, that chemiluminescence of this system is indeed generated by the activity of the cytochrome c with cardiolipin complex, and the method of activated chemiluminescence is actually suitable for its study. The effect of trolox and dihydroquercetin (taxifolin) as synthetic and natural antioxidants on lipoperoxidase activity of the cytochrome c with cardiolipin complex was as well assessed via application of chemiluminescence activator specific for lipid peroxidation reactionscoumarin-334. A complete inhibition of lipoperoxidase activity for a few minutes with its subsequent full development under the trolox response and its dose-dependent uniform decrease under dihydroquercetin effect was obtained. These findings are promising for the future studies on inhibition of lipoperoxidase activity of this nanoparticle by antioxidants in order to inhibit the inappropriate apoptosis. Peroxidase activity of intact mitochondria in the comparative application of two chemiluminescence activators: coumarin-334 and coumarin-525, was also featured.

show abstract

supporting

confidence: 68%

Cytochromecas a facultative enzyme – lipoperoxidase and it’s inhibition by antioxidants

Romodin

Vladimirov

Lysenko

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…In the past, various types of chemical and physical probes were used to enhance the photon emission [31], [32], [33], [34]. Chemical probes are capable to enter in the lipid reaction chain and form excited state by its interaction with the product of lipid peroxidation.…”

Section: Introductionmentioning

confidence: 99%

“…On the opposite side, the physical probes can accept an excitation energy from the excited product of lipid peroxidation such as triplet excited carbonyl ( 3 (C = O) * ) and singlet oxygen ( 1 O 2 ) and emit the photons with a much higher quantum efficiency (hundred and thousand time). Among of the physical probe the fluorescence dye, coumarin was employed as an efficient enhancer of chemiluminescence signal of lipid peroxidation [32]. Alternative approach to enhance chemiluminescence signal was exposure of photosynthetic organisms to high temperature.…”

Section: Introductionmentioning

confidence: 99%

Linoleic Acid-Induced Ultra-Weak Photon Emission from Chlamydomonas reinhardtii as a Tool for Monitoring of Lipid Peroxidation in the Cell Membranes

Prasad

Pospı́šil

2011

PLoS ONE

View full text Add to dashboard Cite

Reactive oxygen species formed as a response to various abiotic and biotic stresses cause an oxidative damage of cellular component such are lipids, proteins and nucleic acids. Lipid peroxidation is considered as one of the major processes responsible for the oxidative damage of the polyunsaturated fatty acid in the cell membranes. Various methods such as a loss of polyunsaturated fatty acids, amount of the primary and the secondary products are used to monitor the level of lipid peroxidation. To investigate the use of ultra-weak photon emission as a non-invasive tool for monitoring of lipid peroxidation, the involvement of lipid peroxidation in ultra-weak photon emission was studied in the unicellular green alga Chlamydomonas reinhardtii. Lipid peroxidation initiated by addition of exogenous linoleic acid to the cells was monitored by ultra-weak photon emission measured with the employment of highly sensitive charged couple device camera and photomultiplier tube. It was found that the addition of linoleic acid to the cells significantly increased the ultra-weak photon emission that correlates with the accumulation of lipid peroxidation product as measured using thiobarbituric acid assay. Scavenging of hydroxyl radical by mannitol, inhibition of intrinsic lipoxygenase by catechol and removal of molecular oxygen considerably suppressed ultra-weak photon emission measured after the addition of linoleic acid. The photon emission dominated at the red region of the spectrum with emission maximum at 680 nm. These observations reveal that the oxidation of linoleic acid by hydroxyl radical and intrinsic lipoxygenase results in the ultra-weak photon emission. Electronically excited species such as excited triplet carbonyls are the likely candidates for the primary excited species formed during the lipid peroxidation, whereas chlorophylls are the final emitters of photons. We propose here that the ultra-weak photon emission can be used as a non-invasive tool for the detection of lipid peroxidation in the cell membranes.

show abstract

“…Excited CO, is proposed to be an emitter of blue-green chemiluminescence for the reactions of reactive oxygen species generated in the xanthinelxanthine oxidase system or in the Fenton reaction (25,26) and may be involved in the peroxynitrite-mediated chemiluminescence. However, chemiluminescence from xanthine/xanthine oxidase (data not shown) and from the Fenton reaction (27) both with and without COz enrichment is not sensitized by C-525; this argues against the contribution of excited C02, at least to the sensitized chemiluminescence in the peroxynitrite system. Moreover, recent calculations predicted a low-energy output (about 15 kcal/mol) in the dismutation of two HCO,' radicals giving two C 0 2 and H202 (W. H. Koppenol, personal communication).…”

Section: Discussionmentioning

confidence: 91%

Sensitization of Peroxynitrite Chemiluminescence by the Triplet Carbonyl Sensitizer Coumarin-525. Effect of CO2

Sharov¹,

Driomina²,

Briviba³

et al. 1998

Photchem. Photbio.

Self Cite

View full text Add to dashboard Cite

The flash of spontaneous chemiluminescence (CL) that reflects the formation of electronically excited intermediates during the decay of peroxynitrite (ONOO-) to nitrate was investigated. The half-decay time of the CL flash (0.5 * 0.1 s) was in agreement with the half-life of peroxynitrite obtained in stopped-flow experiments. The spontaneous CL intensity was linearly dependent on peroxynitrite concentration. The yield of spontaneous CL from peroxynitrite decay, 2 x lo-' photonslperoxynitrite at pH 9.5, was strongly enhanced by a sensitizer of triplet carbonyl CL, coumarin-525 (C-525). The maximal yield of sensitized CL was calculated to be 3 X photons/ peroxynitrite molecule for infinite concentration of C-525. The dependence of both spontaneous and sensitized CL on pH has a maximum at about pH 9.5. Bubbling with C 0 2 or addition of NaHCO, considerably enhanced the flash of CL, and it is concluded that the reaction of the peroxynitrite anion with C 0 2 is a major pathway leading to the formation of an electronically excited intermediate of peroxynitrite.

show abstract

Coumarin derivatives enhance the chemiluminescence accompanying lipid peroxidation

Cited by 35 publications

References 13 publications

Cytochromecas a facultative enzyme – lipoperoxidase and it’s inhibition by antioxidants

Cytochromecas a facultative enzyme – lipoperoxidase and it’s inhibition by antioxidants

Linoleic Acid-Induced Ultra-Weak Photon Emission from Chlamydomonas reinhardtii as a Tool for Monitoring of Lipid Peroxidation in the Cell Membranes

Sensitization of Peroxynitrite Chemiluminescence by the Triplet Carbonyl Sensitizer Coumarin-525. Effect of CO2

Contact Info

Product

Resources

About