Counter-current fractionation-assisted and bioassay-guided separation of active compounds from cranberry and their interaction with α-glucosidase

Tan, Jiaqi; Zhu, Xiaomeng; Li, Qian; Tang, Jintian; Xue, Hongkun

doi:10.1016/j.lwt.2021.111374

Cited by 11 publications

(6 citation statements)

References 42 publications

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“…During the first experiment 47 fractions of 12 mL were collected with a flow rate of 6 mL•min −1 and a rotor spinning at 1000 rpm (Supplementary Figure S2). In these conditions a loss of stationary phase (102 mL) was observed in the first collected tubes (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17) and, consequently, a low retention of stationary phase (59.2%). Flow rate and rotation speed are essential for the fluid dynamics in CPC and can influence the quality of separation [24,29].…”

Section: Centrifugal Partition Chromatography Of the N-butanol Extrac...mentioning

confidence: 99%

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Bioassay-Guided Fractionation of Siparuna glycycarpa n-Butanol Extract with Inhibitory Activity against Influenza A(H1N1)pdm09 Virus by Centrifugal Partition Chromatography (CPC)

et al. 2022

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Siparuna glycycarpa occurs in the Amazon region, and some species of this genus are used in Brazilian folk medicine. A recent study showed the inhibitory effect of this species against influenza A(H1N1)pdm09 virus, and in order to acquire active fractions, a polar solvent system n-butanol-methanol-water (9:1:10, v/v) was selected and used for bioassay-guided fractionation of n-butanol extract by centrifugal partition chromatography (CPC). The upper phase was used as stationary phase and the lower phase as mobile (descending mode). Among the collected fractions, the ones coded SGA, SGC, SGD, and SGO showed the highest antiviral inhibition levels (above 74%) at 100 µg·mL−1 after 24 h of infection. The bioactive fractions chemical profiles were investigated by LC-HRMS/MS data in positive and negative ionization modes exploring the Global Natural Products Social Molecular Networking (GNPS) platform to build a molecular network. Benzylisoquinoline alkaloids were annotated in the fractions coded SGA, SGC, and SGD collected during elution step. Aporphine alkaloids, O-glycosylated flavonoids, and dihydrochalcones in SGO were acquired with the change of mobile phase from lower aqueous to upper organic. Benzylisoquinolinic and aporphine alkaloids as well as glycosylated flavonoids were annotated in the most bioactive fractions suggesting this group of compounds as responsible for antiviral activity.

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Section: Centrifugal Partition Chromatography Of the N-butanol Extrac...mentioning

confidence: 99%

“…Many chromatographic techniques can be employed in this approach. However, depending on the applied technique, some bioactive compounds may be lost due to irreversible adsorption on the chromatographic column, common in solid stationary phase techniques [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Bioassay-Guided Fractionation of Siparuna glycycarpa n-Butanol Extract with Inhibitory Activity against Influenza A(H1N1)pdm09 Virus by Centrifugal Partition Chromatography (CPC)

et al. 2022

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“…Compound 5 displayed a molecular ion m / z 595.17 [M+H] + and a fragment ion at m / z 287.05. This fragment was assumed to be the result of the loss of glucose moiety (162 Da) and rhamnose moiety (146 Da) from the m / z 595.17 [M+H] + , suggesting that the compound was one of the anthocyanins in centaurea cyanus, and when compared with the mass spectrum characteristic found in sour cherry [ 22 ] and cranberry [ 26 ]. Thus, compound 5 was identified as cyanidin-3-rutinoside.…”

Section: Resultsmentioning

confidence: 99%

Phenolics Profile and Protective Effect on Injuried HUVEC Cells of Epicarp Extracts from Kadsura coccinea

Yang

Cheng

et al. 2022

Foods

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This study evaluated the phenolics profile and the antioxidative properties of K. coccinea fruits epicarp. A total of 13 phenolic compounds (six phenolic acids, four anthocyanins, two flavonols, and one flavone) were identified by ultra performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spetrometry(UPLC-QTOF-MS/MS). Two anthocyanins, cyanidin-3-xylosylrutinoside and cyanidin-3-rutinoside, comprise 30.89~74.76% and 13.90~46.17% of the total amount of anthocyanins in K. Coccinea. Cytoprotective effect results evidenced that pretreatment of Human umbilical vein endothelial cells(HUVECs) with Kadsura. coccinea fruits’ epicarp phenolic extracts at the concentrations of 50–200 µg/mL improved the cell viability after exposure to H2O2 significantly, and inhibited malonaldehyde(MDA) and reactive oxygen species(ROS) overproduction, as well as enhancing the content of superoxide dismutase (SOD) and glutathione Reductase (GR. This study proved that K. coccinea is a natural resource of phenolics rich with potential antioxidant ability, which may be valuable for developing nutraceuticals and dietary supplements.

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“…Subsequently, α-glucosidase further degrades them into monosaccharides (for example, glucose) before absorption [17,18]. Inhibition of α-glycosidase activity is, therefore, a therapeutic strategy used to reduce blood glucose levels, making it a crucial target for modulating postprandial hyperglycemia modulation in Type 2 diabetes mellitus [19]. Commonly used α-glucosidase inhibitors for treatment of diabetes include acarbose, miglitol, and daunorubicin.…”

Section: Introductionmentioning

confidence: 99%

Comparative Assessment of In Vitro Xanthine Oxidase and α-Glucosidase Inhibitory Activities of Cultured Cambial Meristematic Cells, Adventitious Roots, and Field-Cultivated Ginseng

Zhang,

Liu,

Chen

et al. 2024

Nutrients

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Panax ginseng, a traditional Chinese medicine with a history spanning thousands of years, faces overexploitation and challenges related to extended growth periods. Tissue-cultured adventitious roots and stem cells are alternatives to wild and field-cultivated ginseng. In this study, we assessed the in vitro xanthine oxidase and α-glucosidase inhibitory activities of saponin extracts among cultured cambial meristematic cells (CMC), adventitious ginseng roots (AGR), and field-cultivated ginseng roots (CGR). The xanthine oxidase (XO) and α-glucosidase inhibitory activities were determined by uric acid estimation and the p-NPG method, respectively. Spectrophotometry and the Folin–Ciocalteu, aluminum nitrate, and Bradford methods were employed to ascertain the total saponins and phenolic, flavonoid, and protein contents. The calculated IC50 values for total saponin extracts against XO and α-glucosidase were 0.665, 0.844, and >1.6 mg/mL and 0.332, 0.745, and 0.042 mg/mL for AGR, CMC, CGR, respectively. Comparing the total saponin, crude protein, and total phenolic contents revealed that AGR > CMC > CGR. To the best of our knowledge, this study presents the first report on the in vitro comparison of xanthine oxidase and α-glucosidase inhibitory activities among AGR, CMC, and CGR. The findings offer valuable insights into the development of hypoglycemic and antihyperuricemic medicinal, nutraceutical, and functional products utilizing AGR and CMC.

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Counter-current fractionation-assisted and bioassay-guided separation of active compounds from cranberry and their interaction with α-glucosidase

Cited by 11 publications

References 42 publications

Bioassay-Guided Fractionation of Siparuna glycycarpa n-Butanol Extract with Inhibitory Activity against Influenza A(H1N1)pdm09 Virus by Centrifugal Partition Chromatography (CPC)

Bioassay-Guided Fractionation of Siparuna glycycarpa n-Butanol Extract with Inhibitory Activity against Influenza A(H1N1)pdm09 Virus by Centrifugal Partition Chromatography (CPC)

Phenolics Profile and Protective Effect on Injuried HUVEC Cells of Epicarp Extracts from Kadsura coccinea

Comparative Assessment of In Vitro Xanthine Oxidase and α-Glucosidase Inhibitory Activities of Cultured Cambial Meristematic Cells, Adventitious Roots, and Field-Cultivated Ginseng

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