Counterimmunoelectrophoresis and latex particle agglutination in the etiologic diagnosis of presumed bacterial pneumonia in pediatric patients

Rusconi, Franca; Rancilio, L.; Bm, Assael; Bonora, Gilberto; Cerri, Michaela; Mc, Pietrogrande; Razon, S; Serafini, Luciano Neder; Torti, G; Vaggi, D.

doi:10.1097/00006454-198811000-00007

Cited by 31 publications

(10 citation statements)

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“…The results found in the simultaneous detection of the capsular polysaccharide antigen of the two bacteria can be ascribed to mixed infection, as reported in some studies. 22,26 Also, it can be attributed to cross-reactivity between the similar antigenic structures of these two bacteria, as described by others, 10,18,27,28 but this seems less probable.…”

Section: Discussionmentioning

confidence: 84%

Antigen detection for the diagnosis of pneumonia

Nunes

Camargos

Costa

et al. 2004

Pediatric Pulmonology

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Streptococcus pneumoniae and Haemophilus influenzae type b are the main agents of bacterial community-acquired pneumonia in developing countries, although a definite etiologic diagnosis cannot be established in most cases. This study was carried out to assess the performance of a latex particle agglutination test (LPAT) from a commercial kit (Slidex Méningite Kit trade mark, BioMérieux, France) in diagnosing pneumococcal and H. influenzae type b pneumonia. One hundred and seven children (45 ill subjects and 62 healthy controls) were enrolled. All 45 cases had a presumptive diagnosis of bacterial pneumonia based on clinical (WHO criteria), laboratory (white blood cell count > or = 15.000/mm3, polymorphonuclear leukocytes > or = 70%, bands > or = 500/mm3, and C-reactive protein > or = 40 mg/l), and radiological findings, i.e., two or more positive points in the scoring system described by Khamapirad and Glezen (Semin Respir Infect 1987;2:130-144). Clinical, laboratory, and radiological assessments were performed in a blinded manner. LPAT was performed in urine samples after concentration through an ethanol-acetone solution. Sensitivity, specificity, and positive and negative predictive values were 77.3% (95% CI, 61.8-88.0%), 90.3% (95% CI, 79.5-96.0%), 85.0% (95% CI, 69.5-93.8%), and 84.8% (95% CI, 73.4-92.1%), respectively. Results suggest that LPAT is a useful diagnostic tool for the etiologic diagnosis of S. pneumoniae and H. influenzae type b pneumonia, especially in the developing world.

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Section: Discussionmentioning

confidence: 84%

Antigen detection for the diagnosis of pneumonia

Nunes

Camargos

Costa

et al. 2004

Pediatric Pulmonology

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“…21 In our study neither clinical features not laboratory markers differed between bacteremic and urinary antigen-diagnosed pneumococcal pneumonia, although there are only five patients with bacteremic pneumonia because it is uncommon in children. Detection of pneumococcal capsular polysaccharide antigen in clinical samples by counterimmunoelectrophoresis has been performed, showing its usefulness for pneumococcal pneumonia 22 During pneumococcal pneumonia it is possible to detect urinary antigen as a result of circulating organisms in the bloodstream and release of pneumococcal antigen from the lung. 23 Elevated PCT values in pneumococcal pneumonia are expected because PCT release depends on bacterial endotoxin and probably other bacterial products.…”

Section: Discussionmentioning

confidence: 99%

Procalcitonin, C-reactive protein and leukocyte count in children with lower respiratory tract infection

Prat

Domínguez

Corrêa-Oliveira

et al. 2003

The Pediatric Infectious Disease Journal

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“…Initially, most of studies planned to evaluate the roles of biomarkers in defining the etiology of pediatric CAP have been carried out using, alone or in combination, the erythrocyte sedimentation rate (ESR), white blood cell (WBC) count, neutrophil percentage (NP), and serum C-reactive protein (CRP) concentration [14,15,16,17,18,19,20,21,22,23,24]. Some years later, serum procalcitonin (PCT) was also included [25,26,27].…”

Section: Differentiation Of Bacterial From Viral Capmentioning

confidence: 99%

Biomarkers in Pediatric Community-Acquired Pneumonia

Principi

Esposito

2017

IJMS

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Community-acquired pneumonia (CAP) is an infectious disease caused by bacteria, viruses, or a combination of these infectious agents. The severity of the clinical manifestations of CAP varies significantly. Consequently, both the differentiation of viral from bacterial CAP cases and the accurate assessment and prediction of disease severity are critical for effectively managing individuals with CAP. To solve questionable cases, several biomarkers indicating the etiology and severity of CAP have been studied. Unfortunately, only a few studies have examined the roles of these biomarkers in pediatric practice. The main aim of this paper is to detail current knowledge regarding the use of biomarkers to diagnose and treat CAP in children, analyzing the most recently published relevant studies. Despite several attempts, the etiologic diagnosis of pediatric CAP and the estimation of the potential outcome remain unsolved problems in most cases. Among traditional biomarkers, procalcitonin (PCT) appears to be the most effective for both selecting bacterial cases and evaluating the severity. However, a precise cut-off separating bacterial from viral and mild from severe cases has not been defined. The three-host protein assay based on C-reactive protein (CRP), tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), plasma interferon-γ protein-10 (IP-10), and micro-array-based whole genome expression arrays might offer more advantages in comparison with former biomarkers. However, further studies are needed before the routine use of those presently in development can be recommended.

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Counterimmunoelectrophoresis and latex particle agglutination in the etiologic diagnosis of presumed bacterial pneumonia in pediatric patients

Cited by 31 publications

References 0 publications

Antigen detection for the diagnosis of pneumonia

Antigen detection for the diagnosis of pneumonia

Procalcitonin, C-reactive protein and leukocyte count in children with lower respiratory tract infection

Biomarkers in Pediatric Community-Acquired Pneumonia

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