Counterion binding to protamine polyion at a polarised liquid–liquid interface

Trojánek, Antonı́n; Langmaier, Jan; Samcová, Eva; Samec, Zdeněk

doi:10.1016/j.jelechem.2007.02.006

Cited by 41 publications

(45 citation statements)

References 32 publications

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“…Trojanek et al 42 reported a comprehensive study of counter anion binding to protamine at there was no appearance of pre-peaks as reported by Amemiya et al 37 and as have been seen for studies with larger proteins.…”

Section: Protaminementioning

confidence: 58%

Voltammetry of proteins at liquid–liquid interfaces

Arrigan

2013

Annu. Rep. Prog. Chem., Sect. C: Phys. Chem.

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Section: Protaminementioning

confidence: 58%

Voltammetry of proteins at liquid–liquid interfaces

Arrigan

2013

Annu. Rep. Prog. Chem., Sect. C: Phys. Chem.

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“…The peak on the reverse scan (toward positive potential) is then attributed to a desorption process encompassing the dissociation of the complex formed between the polyanionic U. pinnatifida fucoidan and the organic electrolyte cation. Counterion-polyion interactions have been observed in several polyelectrolyte systems at the ITIES, 43,44,58 and may reflect a generic mechanism for the electrochemical detection of polyionic analytes. CVs of increasing concentrations (10-1000 μg mL ( Fig.…”

Section: -53mentioning

confidence: 94%

Electrochemical behaviour at a liquid-organogel microinterface array of fucoidan extracted from algae

Felisilda

Eulate

Stringer³

et al. 2017

Analyst

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a Fucoidans are sulfated polysaccharides mostly derived from algae and used in a number of applications (e.g. nutrition, cosmetics, pharmaceuticals and biomaterials). In this study, the electrochemical behaviour of fucoidans extracted from two algal species (Undaria pinnatifida and Fucus vesiculosus) was assessed using voltammetry at an array of micro-interfaces formed between two immiscible electrolyte solutions (μITIES) in which the organic electrolyte phase was gelled. Cyclic voltammetry revealed an adsorption process when scanning to negative potentials, followed by a desorption peak at ca. −0.50 V on the reverse scan, indicating the electroactivity of both fucoidans. U. pinnatifida fucoidan showed a more intense voltammetric signal compared to F. vesiculosus fucoidan. In addition, use of tridodecylmethylammonium (TDMA + ) or tetradodecylammonium (TDDA + ) as the organic phase electrolyte cation provided improved detection of both fucoidans relative to the use of bis (triphenylphosphoranylidene) phase and adsorptive pre-concentration for 180 s afforded a detection limit of 1.8 μg mL −1 fucoidan (U. pinnatifida) in aqueous phase of 10 mM NaOH and 2.3 μg mL −1 in synthetic urine ( pH adjusted). These investigations demonstrate the electroactivity of fucoidans at the μITIES array and provide scope for their detection at low μg mL −1 concentrations using this approach.

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“…In recent years there have been many reports on the behaviour and detection of biomolecules at the ITIES. The detection of a range of biomolecules including amino acids [19], heparin [20,21], protamine [22,23], haemoglobin [24,25], lysozyme [26,27], insulin [28], dopamine [29][30][31], noradrenaline [31] and DNA [32] have been reported at the ITIES.…”

Section: Introductionmentioning

confidence: 99%

“…A range of proteins have been studied at the ITIES in the absence of surfactants and it was found that the proteins did not transfer across the ITIES but adsorbed there and facilitated the transfer of the organic anion to the aqueous phase [24,[26][27][28]. The effect of varying the organic phase anion on the electrochemistry of protamine at the ITIES was extensively studied by Trojanek et al using conductometry, voltammetry and quasi-elastic light scattering [22]. The interactions of cationic proteins with the hydrophobic anions has been verified also by on-line acoustic sensor [36] and mass spectrometric [37] methods.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical behaviour of myoglobin at an array of microscopic liquid–liquid interfaces

O'Sullivan

Arrigan

2012

Electrochimica Acta

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Electrochemistry at liquid-liquid interfaces, or at interfaces between two immiscible electrolyte solutions (ITIES), provides a basis for the non-redox detection of biological molecules, based on iontransfer or adsorption processes. The electroactivity of myoglobin at an array of micron-sized liquidorganogel interfaces was investigated. The µITIES array was patterned with a silicon membrane consisting of an array of eight pores with radii of ~12.8 µm and a pore to pore separation of ~400 µm.Using cyclic voltammetry at the ITIES, the protein was shown to adsorb at the interface and facilitate the transfer of the organic phase electrolyte anions to the aqueous side of the interface. The electrochemical current response was linear with concentration in the range of 1 -6 µM, with corresponding surface coverage of 10 -50 pmol cm -2 . The reverse peak currents was found to be proportional to the voltammetric scan rate, indicating a desorption process. The detection of the protein was only possibly when the pH of the aqueous phase solution was below the pI of the protein. The steady-state simple ion transfer behaviour of tetraethylammonium cation was decreased on the forward sweep, providing a qualitative indication of the presence of adsorbed protein at the interface.Increasing the ionic strength of the aqueous phase resulted in enhanced peak currents, possibly due to aggregation of protein precipitates in the aqueous solution. UV/Vis absorbance spectroscopy was used 1 ISE Member.2 | P a g e to investigate the effects of various aqueous electrolyte solutions on the structure of the protein, and it was shown that at low pH the protein is at least partially denatured. These results provide the basis for label-free detection of myoglobin at the ITIES.

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Counterion binding to protamine polyion at a polarised liquid–liquid interface

Cited by 41 publications

References 32 publications

Voltammetry of proteins at liquid–liquid interfaces

Voltammetry of proteins at liquid–liquid interfaces

Electrochemical behaviour at a liquid-organogel microinterface array of fucoidan extracted from algae

Electrochemical behaviour of myoglobin at an array of microscopic liquid–liquid interfaces

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