Counterion effects in protein nanoparticle electrostatic binding: A theoretical study

Ghosh, Goutam

doi:10.1016/j.colsurfb.2015.02.015

Cited by 9 publications

(6 citation statements)

References 35 publications

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“…4a and b, the increase of IS (from 0.01 M to 0.3 M) leads to the RMSD of adsorbed lysozyme slightly decreasing for the same size SNP, since it might screen the electrostatic attraction between adsorbed lysozyme and different-sized SNPs and thus cause a reduction of conformation loss. 54 Meanwhile, Ghosh et al 55,56 suggested that Na + can unfold proteins. Since there are more Na + ions in systems of strongly negatively charged SNPs, an interfacial Na + layer can be formed independently without the increase of IS.…”

Section: Resultsmentioning

confidence: 99%

“…The essential reason for this phenomenon is that the interfacial Na + layer formed around strongly negatively charged SNP surfaces can weaken the effect of IS, although it would not be favorable for keeping lysozyme conformation. 55,56 Another possible reason is that Na + tends to stay in the bulk, 63,64 although there is a strong electrostatic attraction between Na + and the strongly negatively charged SNPs. Therefore, for the system of SNPs, the effect of IS is not obvious for the formation of interfacial hydration layer, and it might be the internal reason for the increase of solution IS not significantly affecting adsorption behavior, which was observed in previous experiments also.…”

Section: Resultsmentioning

confidence: 99%

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Understanding the curvature effect of silica nanoparticles on lysozyme adsorption orientation and conformation: a mesoscopic coarse-grained simulation study

Jian

2016

Phys. Chem. Chem. Phys.

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In nanobiotechnology applications, curvature of nanoparticles has a significant effect on protein activities. In this work, lysozyme adsorption on different-sized silica nanoparticles (SNPs) was simulated at the microsecond timescale by using mesoscopic coarse-grained molecular dynamics simulations. It is found that, with the increase of nanoparticle size, which indicates a decrease of surface curvature, adsorbed lysozyme shows a narrower orientation distribution and a greater conformation change, as the electrostatic attraction dominates lysozyme adsorption, and this trend is more pronounced on larger SNPs. Interestingly, the effect induced by different SNP surface curvatures is not related to the direct contact area between lysozyme and SNPs, but to the interfacial hydration layer above the silica surface, since a smaller curvature can lead to a stronger interfacial hydration and make the distribution of interfacial water molecules more ordered. Besides, at higher ionic strength, lysozyme conformation is less affected by strongly negatively charged SNPs, especially for larger nanoparticles. This work might shed some light on how to prepare protein coronas with higher bioactivities in nanobiotechnology.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Understanding the curvature effect of silica nanoparticles on lysozyme adsorption orientation and conformation: a mesoscopic coarse-grained simulation study

Jian

2016

Phys. Chem. Chem. Phys.

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“…Based on our experimental results, it was evident that only CPC coated IONPs were toxic to Raji cancer cells which could be realized using the RCPC model [35]: (i) electrostatic binding of CPC-IONPs with negative components of the cell membrane and (ii) subsequent release of counterions (Cl − ) and unfolding of proteins or lipids in the membrane which caused fragmentation and cell death (Figs. 3(C) and (D)).…”

Section: Discussionmentioning

confidence: 94%

“…Electrostatic binding of proteins causes counterions to release from the charged coating of nanoparticles and diffuse into interior of bound proteins, disrupt their hydrophobic core and hydrogen bonds to unfold [34]. This model of unfolding of proteins was named the reverse charge parity counterions (RCPC) model [35] and has been observed to work successfully for different proteins [36,37]. This model thus describes the selective toxicity of counterions-conjugated charged IONPs to proteins.…”

Section: Introductionmentioning

confidence: 99%

Selective Cytotoxicity of Counterion-Conjugated Charged Iron Oxide Nanoparticles: A Study with Lymphoblastoid Raji Cells

Ghosh¹,

Mukherjee²,

Bhilwade³

et al. 2018

JAN

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The cytotoxicity of counterions-conjugated charged iron oxide nanoparticles (IONPs) was studied with lymphoblastoid Raji cells and was compared with peripheral blood lymphocytes. IONPs were coated either with tri-potassium citrate (TKC), or with cetylpyridinium chloride (CPC). TKC coated IONPs were negatively charged and K + counterions conjugated nanoparticles, and CPC coated IONPs were positively charged Clcounterions conjugated nanoparticles. The cells were incubated with IONPs at 37 °C for 24 h and the cytotoxicity was studied by measuring the cell viability using MTT and LDH assays. The cytotoxicity of IONPs was further assessed through DNA fragmentation assay. Morphology of Raji cells was also observed by TEM. We have used a modified membrane lysis model to understand the cell death via cell membrane lysis due to counterions diffusion upon binding of IONPs.

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“…Huang et al have reported the effects of surface compositional and structural heterogeneity on protein adsorption by examining the interaction of self-assembled monolayer coated gold NPs (AuNPs) with two types of proteins: ubiquitin and fibrinogen [150]. The surface coating on nanoparticles with charged and neutral molecules shows different affinities to the binding of proteins and it causes irreversible conformational unfolding of proteins if counterions are present in the coating molecules [151][152][153][154][155][156]. Corona alters the size and interfacial composition of a nanoparticle, giving it a distinct 'biological identity' from that of the original one [157,158].…”

Section: Protein-nanoparticle Interactions and Signaling Pathways In Cancer Cellsmentioning

confidence: 99%

Aberrant Signaling Pathways in Cancer Cells: Application of Nanomaterials

2021

Journal of Cellular Signaling

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Cell signaling pathways involve several proteins, called kinases, to pass on vital messages from the transmembrane-proteins, e.g., receptor tyrosine kinases (RTKs) to the nucleus of a cell through a cascade of processes for various activities such as controlled cell division, proliferation, apoptosis, metabolism, DNA replication and repair. Alterations in these signaling proteins combined with genetic and/or epigenetic mutations spawn various types of cancer that empowers the cells to ignore or maneuver the immune signals and carry on felonious activities such as uncontrolled cell division and growth, genetic instability, angiogenesis around the tumor in hypoxia, eluding the body's immune system, cell migration, and invasion of surrounding healthy cells. Inhibition of such malicious transmuted signaling pathways has therapeutic importance for cancer. In this article, we have briefly described three vital signaling pathways that are aberrantly triggered by Ras and Wnt proteins and the NF-2 genes causing different types of cancer. Recent research in the targeted therapy using drugs to put off these signaling pathways, especially, for cancer stem cells (CSCs) has been reviewed here. However, such therapy suffers a low success rate due to the resistance to drugs by cancer cells. Considering this snag of targeting drugs, many researchers use nanoparticles for this purpose; some of these works have been reviewed in brief. The consequences of protein-nanoparticles interaction in this approach has also been discussed. Finally, we have proposed use of nanomaterials for targeted ion delivery, or targeted heating using light or magnetic field, to destroy cancer cells.

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Counterion effects in protein nanoparticle electrostatic binding: A theoretical study

Cited by 9 publications

References 35 publications

Understanding the curvature effect of silica nanoparticles on lysozyme adsorption orientation and conformation: a mesoscopic coarse-grained simulation study

Understanding the curvature effect of silica nanoparticles on lysozyme adsorption orientation and conformation: a mesoscopic coarse-grained simulation study

Selective Cytotoxicity of Counterion-Conjugated Charged Iron Oxide Nanoparticles: A Study with Lymphoblastoid Raji Cells

Aberrant Signaling Pathways in Cancer Cells: Application of Nanomaterials

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