Counting and Recognizing Single Bacterial Cells by a Lanthanide-Encoding Inductively Coupled Plasma Mass Spectrometric Approach

Liang, Yong; Liu, Qian; Zhou, Yang; Shi, Chen; Yang, Limin; Zhu, Min; Wang, Qiuquan

doi:10.1021/acs.analchem.9b01130

Cited by 48 publications

(25 citation statements)

References 51 publications

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“…Nowadays, this technique has been proven to be a versatile tool for studies on the determination of constitutive elements, the cellular uptake of metal-containing drugs, − and the incorporation of metallic nanoparticles . In combination with labeling strategies, further studies have shown the versatility of this technique in characterizing individual cells or bacteria. − The development of novel elemental tags that allow increased sensitivity is currently an active field of research. Besides polymers or metal nanoparticles, other nanostructures can be used to allocate a large number of tag-element atoms, such as viruslike nanoparticles …”

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confidence: 99%

Quantitative Analysis of Transferrin Receptor 1 (TfR1) in Individual Breast Cancer Cells by Means of Labeled Antibodies and Elemental (ICP-MS) Detection

et al. 2019

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Cells are able to precisely control the amount of iron they acquire in the form of transferrin (TF)-bound iron by modulating the synthesis of transferrin receptor 1 (TfR1). In tumor cells, elevated TfR1 seems to be related to poorer outcome for patients. Thus, the direct measurement of this biomarker in breast cancer tissues and cells might serve as a prognosis biomarker. In this work, we have used Nd-labeled antibodies to tag the TfR1 present on the cell surface of two cell models of breast cancer with different malignancy (MCF7 and MDA-MB 231). For this aim, monoclonal antibody anti-TfR1 is first labeled with a polymeric chelator (MAXPAR) with the subsequent incorporation of several isotopic 143 Nd atoms. The characterization of the labeled antibody revealed a stoichiometry of 21 Nd atoms per antibody molecule that can be used for further quantification experiments. This antibody is used for cell tagging followed by single-cell analysis using inductively coupled plasma mass spectrometry (ICP-MS) detection. In this case, cell introduction is conducted using a high-efficiency nebulizer and spray chamber to achieve transport efficiencies of up to 55% for cells. Quantitative results revealed a number of receptors per cell significantly higher in the case of the most malignant phenotype (MDA-MB-231). Absolute and relative TfR1 concentration values are obtained in individual cells for the first time using the proposed system.

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confidence: 99%

Quantitative Analysis of Transferrin Receptor 1 (TfR1) in Individual Breast Cancer Cells by Means of Labeled Antibodies and Elemental (ICP-MS) Detection

et al. 2019

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“…These low-abundance substances often play important roles in their corresponding chemical or biological systems (Chen et al, 2016;Finn et al, 2020;Marabelle et al, 2020). Accurate and sensitive analysis of these low-abundance substances is essential for the in-depth interpretation of related chemical and biological phenomena (Sonnett et al, 2018;Yin et al, 2018;Liang et al, 2019). Therefore, there are great demands for analytical instruments with higher sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Towards Higher Sensitivity of Mass Spectrometry: A Perspective From the Mass Analyzers

Li¹,

Chu²,

Tan³

et al. 2021

Front. Chem.

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Mass spectrometry (MS) is one of the most widely used analytical techniques in many fields. Recent developments in chemical and biological researches have drawn much attention to the measurement of substances with low abundances in samples. Continuous efforts have been made consequently to further improve the sensitivity of MS. Modifications on the mass analyzers of mass spectrometers offer a direct, universal and practical way to obtain higher sensitivity. This review provides a comprehensive overview of the latest developments in mass analyzers for the improvement of mass spectrometers’ sensitivity, including quadrupole, ion trap, time-of-flight (TOF) and Fourier transform ion cyclotron (FT-ICR), as well as different combinations of these mass analyzers. The advantages and limitations of different mass analyzers and their combinations are compared and discussed. This review provides guidance to the selection of suitable mass spectrometers in chemical and biological analytical applications. It is also beneficial to the development of novel mass spectrometers.

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“…Herein, to guarantee the accurate homogeneous immunoassay, a self-validated immunoassay was proposed by simultaneously scrutinizing both the frequency and the intensity of the pulse signal from single gold nanoparticles (AuNPs). The metal isotopes inside nanoparticle tags were sensitively and selectively detected by inductively coupled plasma mass spectrometry (ICPMS), − which has been recently successfully demonstrated in the analysis of metals, , proteins, − nucleic acid, , enzymes, single cells, − etc. The frequency of the pulse signals represents the number concentration of AuNPs, while the recorded signal intensity represents the size of AuNPs. , Carcinoembryonic antigen (CEA), a 180 kDa glycoprotein which belongs to tumor-associated biomarkers in human serum, was selected as a model analyte.…”

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confidence: 99%

Self-Validated Homogeneous Immunoassay by Single Nanoparticle in-Depth Scrutinization

et al. 2020

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The most convenient method for the clinical routine analysis of disease biomarkers is homogeneous immunoassay, which minimizes the requirements for automation and time-/lab-consumption. Despite great success, because sample constituents are not removed by a separation or washing step, a major challenge in conducting homogeneous immunoassays for the practical application is the matrix effect-related inaccuracy. Herein, to guarantee an accurate quantification, a self-validated homogeneous immunoassay was proposed, by simultaneously scrutinizing both frequency and intensity of single gold nanoparticles. The two analytical modes of single particle inductively coupled plasma mass spectrometry (ICPMS) correlated well with each other, resulting in a self-validation mechanism for the accurate immunoassay. Both two modes of the proposed method provided linear ranges of 2 orders of magnitude and LODs of pM level. Thanks to the self-validated strategy and the high tolerance of the matrix effect of ICPMS, the proposed homogeneous immunoassay was successfully demonstrated in a series of human serum samples, with results in good accordance with clinical routine methods.

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Counting and Recognizing Single Bacterial Cells by a Lanthanide-Encoding Inductively Coupled Plasma Mass Spectrometric Approach

Cited by 48 publications

References 51 publications

Quantitative Analysis of Transferrin Receptor 1 (TfR1) in Individual Breast Cancer Cells by Means of Labeled Antibodies and Elemental (ICP-MS) Detection

Quantitative Analysis of Transferrin Receptor 1 (TfR1) in Individual Breast Cancer Cells by Means of Labeled Antibodies and Elemental (ICP-MS) Detection

Towards Higher Sensitivity of Mass Spectrometry: A Perspective From the Mass Analyzers

Self-Validated Homogeneous Immunoassay by Single Nanoparticle in-Depth Scrutinization

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