Countrywide cross-sectional study of swine brucellosis sero-prevalence in Indian subcontinent during 2018–2019

Shome, Rajeswari; Kalleshamurthy, Triveni; Nagaraj, Chaitra; Rathore, Yashaswini; Ramanjinappa, Kavana D.; Skariah, Somy; Nagalingam, M.; Shome, Bibek Ranjan; Chanda, Mohammed Mudassar; Hemadri, D.

doi:10.1007/s11250-022-03107-9

Cited by 3 publications

(1 citation statement)

References 24 publications

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“…Brucellosis is a serious and often neglected zoonosis caused by Brucella infection through contacting the contaminated animal and its products or air dusts . Livestock such as cattle, goats, sheep, camels, and pigs is the most common intermediate hosts of Brucellae , and humans, as the ultimate host, get sick through direct or indirect contact with infected animals or eating contaminated animal products . Around the world, 500,000 people are diagnosed with Brucella infection every year .…”

Section: Introductionmentioning

confidence: 99%

Novel Vertical Flow Immunoassay with Au@PtNPs for Rapid, Ultrasensitive, and On-Site Diagnosis of Human Brucellosis

Zhang

et al. 2023

ACS Omega

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Brucellosis is an infectious zoonosis caused by Brucella with clinical symptoms of wavy fever, fatigue, and even invasion of tissues and organs in the whole body, posing a serious threat to public health around the world. Herein, a novel vertical flow immunoassay based on Au@Pt nanoparticles (Au@PtNPs-VFIA) was established for detection of Brucella IgG antibody in clinical serum samples. The testing card of Au@PtNPs-VFIA was manufactured by printing the purified Brucella LPS and goat antimouse IgG on the nitrocellulose membrane as the test-spot or control-spot, respectively. Au@PtNPs labeled with protein G (Au@PtNPs-prG) were concurrently employed as detection probes presenting visible spots and catalysts mimicking catalytic enzymes to catalyze the DAB substrate (H2O2 plus O-phenylenediamine) for deepening color development. The testing procedure of Au@PtNPs-VFIA takes 2–3 min, and the limit of detection (LOD) for Brucella antibody is 0.1 IU/mL, which is faster and more sensitive than that of Au@PtNP-based lateral flow immunoassay (Au@PtNPs-LFIA: 15 min and 1.56 IU/mL, respectively). By comparing with vertical flow immunoassay based on classic Au nanoparticles (AuNPs-VFIA), the Au@PtNPs-VFIA is 32 times or 16 times more sensitive with or without further development of DAB substrate catalysis. Au@PtNPs-VFIA did not react with the serum samples of Gram-negative bacterium infections but only weakly cross-reacted with diagnostic serum of Y. enterocolitica O9 infection. In detection of clinical samples, Au@PtNPs-VFIA was validated for possessing 98.33% sensitivity, 100% specificity, and 99.17% accuracy, which were comparable with or even better than those obtained by the Rose-Bengal plate agglutination test, serological agglutination test, AuNPs-VFIA, and Au@PtNPs-LFIA. Therefore, this newly developed Au@PtNPs-VFIA has potential for rapid, ultrasensitive, and on-site diagnosis of human Brucellosis in clinics.

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Section: Introductionmentioning

confidence: 99%

Novel Vertical Flow Immunoassay with Au@PtNPs for Rapid, Ultrasensitive, and On-Site Diagnosis of Human Brucellosis

Zhang

et al. 2023

ACS Omega

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Serological, bacteriological, and molecular detection of brucellosis in pigs of Tamil Nadu, India

Preena,

Ronald,

Balakrishnan

et al. 2024

Emerging Animal Species

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Molecular characterization of Brucella species detected from clinical samples of cattle and buffaloes

THORAT¹,

BANNALIKAR²

2022

Indian J of Anim Sci

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The present study was undertaken for molecular characterization of Brucella species of cattle and buffaloes. Clinical samples (1145) of unvaccinated cattle and buffaloes (200 blood samples, 710 sera, 190 vaginal swabs, 20 abomasal contents of foetus, 25 foetal tissues) and 146 blood samples of vaccinated animals were collected from dairy farms in and around Mumbai and Pune region. These samples were processed for isolation of Brucella organisms and further characterized by PCR and sequencing. A total of 26 (11.06%) Brucella isolates were recovered from 235 samples. Also, 5 isolates received from human cases were included in the study. BCSP 31 PCR showed an amplicon of 223 bp in all 31 isolates, 123 (61.5%) blood samples, 123 (64.73%) vaginal swabs and 27 (60%) aborted foetal material. IS711/AB and BM PCR showed an amplicon of 498 bp and 731 bp in 17 and 14 isolates, 42 (21%) and 38 (19%) blood samples, 43 (22.63%) and 34 (17.89%) vaginal swabs, while 7(15.55%) and 6 (13.33%) aborted foetal material, respectively. The phylogenetic analysis detected the ancestral origin of the organism. Rapid and correct diagnosis of brucellosis and vaccination is important to eradicate the disease. The molecular methods used in the present study speed up the diagnosis of the disease.

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Countrywide cross-sectional study of swine brucellosis sero-prevalence in Indian subcontinent during 2018–2019

Cited by 3 publications

References 24 publications

Novel Vertical Flow Immunoassay with Au@PtNPs for Rapid, Ultrasensitive, and On-Site Diagnosis of Human Brucellosis

Novel Vertical Flow Immunoassay with Au@PtNPs for Rapid, Ultrasensitive, and On-Site Diagnosis of Human Brucellosis

Serological, bacteriological, and molecular detection of brucellosis in pigs of Tamil Nadu, India

Molecular characterization of Brucella species detected from clinical samples of cattle and buffaloes

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