Coupled-Column Liquid Chromatographic Analysis of Catecholamines, Serotonin, and Metabolites in Human Urine

Panholzer, Torsten J.; Beyer, Jürgen; Lichtwald, K.

doi:10.1093/clinchem/45.2.262

Cited by 74 publications

(24 citation statements)

References 11 publications

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“…The concentrations of NE, E, and DA in the urine samples are in the range of 17–37, 11–15, and 39–105 ng/mL, respectively. The results are accordant with the literature values . The results demonstrated that proposed method is feasible for the analysis of CAs in urine.…”

Section: Resultssupporting

confidence: 89%

Determination of catecholamines in urine using aminophenylboronic acid functionalized magnetic nanoparticles extraction followed by high-performance liquid chromatography and electrochemical detection

et al. 2015

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A new method was developed for the simultaneous determination of three catecholamines in urine using aminophenylboronic acid functionalized magnetic nanoparticles extraction followed by high-performance liquid chromatography with electrochemical detection. Novel aminophenylboronic acid functionalized magnetic nanoparticles were prepared by multi-step covalent modification, and characterized by transmission electron microscopy, Fourier-transformed infrared spectroscopy, X-ray diffraction, and vibrating sample magnetometry. With the help of the high affinity between the boronate and cis-diol group, the particles were used for the highly selective separation and enrichment of three major catecholamines, norepinephrine, epinephrine, and dopamine. Effects of the pH of the feed solution, the extraction time, the composition of the buffer solution, the amount of the magnetic particles, the elution conditions, and the recycling of aminophenylboronic acid functionalized magnetic nanoparticles were explored. Under the optimized conditions, 13-17-fold enrichment factors were obtained. The linear ranges were 0.01-2.0 μg/mL for the studied analytes. The limits of detection and quantification were in the range of 2.0-7.9 and 6.7-26.3 ng/mL, respectively. The relative recoveries were in the range of 92-108%, with intraday and interday relative standard deviations lower than 6.8%. This method was successfully applied to analysis of catecholamines in real urine.

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Section: Resultssupporting

confidence: 89%

Determination of catecholamines in urine using aminophenylboronic acid functionalized magnetic nanoparticles extraction followed by high-performance liquid chromatography and electrochemical detection

et al. 2015

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“…Since the 1960s, several studies depended upon biomarkers of neurotransmitters and metabolites [14]. C. Several studies concluded that there is a direct correlation between urine and CNS neurotransmitters [15][16][17][18]. Many researchers and clinicians are formally using urinary neurotransmitters as biomarkers and as a diagnostic and assessment tool [15,[17][18][19].…”

Section: Introductionmentioning

confidence: 99%

“…Activation of norepinephrine raises alertness, preparing the body for action by increasing heart rate and blood pressure, mobilizing glucose, and facilitating blood flow to skeletal muscles. If the stress continues or increases, norepinephrine is converted to epinephrine [17,[25][26][27].…”

Section: Introductionmentioning

confidence: 99%

The Impact of MNRI Therapy on the Levels of Neurotransmitters Associated with Inflammatory Processes

Tatarinova

Deiss²,

Franckle

et al. 2020

IJMS

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The neurotransmitter levels of representatives from five different diagnosis groups were tested before and after participation in the MNRI®—Masgutova Neurosensorimotor Reflex Intervention. The purpose of this study was to ascertain neurological impact on (1) Developmental disorders, (2) Anxiety disorders/OCD (Obsessive Compulsive Disorder), PTSD (Post-Traumatic Stress disorder), (3) Palsy/Seizure disorders, (4) ADD/ADHD (Attention Deficit Disorder/Attention Deficit Disorder Hyperactive Disorder), and (5) ASD (Autism Spectrum Disorder) disorders. Each participant had a form of neurological dysregulation and typical symptoms respective to their diagnosis. These diagnoses have a severe negative impact on the quality of life, immunity, stress coping, cognitive skills, and social assimilation. This study showed a trend towards optimization and normalization of neurological and immunological functioning, thus supporting the claim that the MNRI method is an effective non-pharmacological neuromodulation treatment of neurological disorders. The effects of MNRI on inflammation have not yet been assessed. The resulting post-MNRI changes in participants’ neurotransmitters show significant adjustments in the regulation of the neurotransmitter resulting in being calmer, a decrease of hypervigilance, an increase in stress resilience, behavioral and emotional regulation improvements, a more positive emotional state, and greater control of cognitive processes. In this paper, we demonstrate that the MNRI approach is an intervention that reduces inflammation. It is also likely to reduce oxidative stress and encourage homeostasis of excitatory neurotransmitters. MNRI may facilitate neurodevelopment, build stress resiliency, neuroplasticity, and optimal learning opportunity. There have been no reported side effects of MNRI treatments.

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“…Early days concerns about the limitations of urinalysis for neurotransmitters, were alleviated by development of fluorometric methods. High-Performance Liquid Chromatography (HPLC) methodology has expanded parameters of even greater specificity and sensitivity, allowing an even wider span of clinical application [31] [32] [33]. Lynn-Bullock examined the effects of oral ingestion of the serotonin substrate (5-hydroxytryptophan (5-HTP)) on specific brain regions in rats [34].…”

Section: Introductionmentioning

confidence: 99%

The Effect of the MNRI Method on Neurotransmitter Biomarkers of Individuals with Neurodevelopmental Disorders

Bell¹,

Whitney²,

Deiss³

et al. 2019

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Introduction: The MNRI (Masgutova Neurosensorimotor Reflex Integration) method was developed in 1989 in Russia and has spread worldwide to treat individuals with certain types reflex development deficits, behavior disorders, disorders of speech or language development, and learning disabilities. MNRI is based on techniques called "repatterning" or remodulation, meaning re-education, recoding the reflex nerve pathways specific for dynamic and postural reflex schemes. Objectives: Repatterning activates the extra pyramidal nervous system responsible for automatic mechanisms and processes, the extension of links between neurons, the growth of neural nets, myelination, and the creation of new nerve routing. This potential result was tested utilizing urinary measurements of the following neurotransmitters:

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Coupled-Column Liquid Chromatographic Analysis of Catecholamines, Serotonin, and Metabolites in Human Urine

Cited by 74 publications

References 11 publications

Determination of catecholamines in urine using aminophenylboronic acid functionalized magnetic nanoparticles extraction followed by high-performance liquid chromatography and electrochemical detection

Determination of catecholamines in urine using aminophenylboronic acid functionalized magnetic nanoparticles extraction followed by high-performance liquid chromatography and electrochemical detection

The Impact of MNRI Therapy on the Levels of Neurotransmitters Associated with Inflammatory Processes

The Effect of the MNRI Method on Neurotransmitter Biomarkers of Individuals with Neurodevelopmental Disorders

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