In this paper, some fixed point theorems for monotone operators in partially ordered complete metric spaces are proved. Especially, a sufficient and necessary condition for the existence of a fixed point for a class of monotone operators is presented. The main results of this paper are generalizations of the recent results in the literature. Also, the main results can be applied to solve the nonlinear elliptic problems and the delayed hematopoiesis models. MSC: 47H10; 54H25