Coupled Luminescent Methods in Drug Discovery: 3‐min Assays for Cytotoxicity and Phosphatase Activity

Abstract

Coupled luminescent technology has wide potential in drug discovery process. In comparison of fluorescent assay, coupled luminescent assays offer very rapid and sensitive readouts with minimal sample and reagent usage. However, there are idiosyncrasies and limitations inherent to the method, many of which are associated with the presence of multiple catalytic activities. This article focuses on the role of DeathTRAK and PhosTRAK assay methods in drug discovery.