Coupling Azo Dye Methods for Histochemical Demonstration of Alkaline Phosphatase

Grogg, E; Pearse, A. G. E.

doi:10.1038/170578b0

Cited by 35 publications

(5 citation statements)

References 2 publications

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“…These enzymes catalyze reversible transamination and products were measured at 570 nm. ALP enzyme assay kit (Abcam, ab83369) uses p-nitrophenyl phosphate (pNPP) as a substrate which turns yellow measured at 405 nm (Grog and Pearse, 1952). LDH enzyme activity is determined by measuring the absorbance at 340 nm resulting from the oxidation of NADH.…”

Section: Methodsmentioning

confidence: 99%

ZnO nanoparticles augment ALT, AST, ALP and LDH expressions in C2C12 cells

Pandurangan

Kim

2015

Saudi Journal of Biological Sciences

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The present study aimed to investigate the effect of ZnO nanoparticles on alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) enzyme expressions in C2C12 cells. ZnO nanoparticles are widely used in the several cosmetic lotions and other biomedical products. Several studies report on ZnO nanoparticle mediated cytotoxicity. However, there are no reports on the effect of ZnO nanoparticles on ALT, AST, ALP and LDH enzyme expressions in C2C12 cells. A cytotoxicity assay was carried out to determine the effect of ZnO nanoparticles (1-5 mg/ml) on C2C12 cell viability at 48 and 72 h. ZnO nanoparticles increased ALT, AST, ALP and LDH enzyme mRNA expression and their activities in C2C12 cells. In conclusion, the present study showed that ZnO nanoparticles increased these enzyme activities and its mRNA expression in C2C12 cells in a dose-dependent manner.

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Section: Methodsmentioning

confidence: 99%

ZnO nanoparticles augment ALT, AST, ALP and LDH expressions in C2C12 cells

Pandurangan

Kim

2015

Saudi Journal of Biological Sciences

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“…The slides were incubated by the method of Grogg and Pearse (1952) and Pearse (1953) at room temperature for 15 to 30 min. in freshly-made solution containing 1 mg. sodium a-naphthylphosphate and 1 mg. 5-chloro-o-toluidine diazotate per 1 ml.…”

Section: Methodsmentioning

confidence: 99%

“…There also exists a tissue affinity for cobalt salts, so that the cobalt * Recived for publication June 16, 1933. salts may not only react with the calcium phosphate but may also be attached directly to some tissue structures. In order to avoid these difficulties, Gomori (1951) devised a new method of phosphatase staining, subsequently modified by Grogg and Pearse (1952). This new method consists in using a-naphthylphosphate in the presence of a diazonium compound.…”

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confidence: 99%

Histochemical Localization of Alkaline Phosphatase in the Retina

Reis¹

1954

British Journal of Ophthalmology

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“…20 Since the ceramic artificial bone used in this study primarily consists of hydroxyapatite, ALP (azo coupling method) was employed for determination. 21 The osteogenic differentiation of rBMSCs on various scaffolds was evaluated through ALP staining after 3 and 5 days of incubation. After 3 days, a blue precipitate was observed on the surface of all groups, with the staining area gradually increasing and becoming denser as the incubation time extended.…”

Section: B In Vitro Osteogenic Studies Of Drug-loaded Artificial Scaf...mentioning

confidence: 99%

Preparation and in vitro osteogenic evaluation of high-strength ceramic artificial bone loaded with anti-tuberculosis drug PaMZ

Li,

Fei,

Zhang

et al. 2023

AIP Advances

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The objective of this study was to prepare a high-strength ceramic artificial bone loaded with the anti-tuberculosis drug PaMZ (delamanid, moxifloxacin, and pyrazinamide) and evaluate its physical characteristics and osteogenic potential. We utilized 3D printing technology to fabricate artificial bones and then obtained a high-strength ceramic artificial bone by high-temperature firing. Then, a triple combination of anti-tuberculosis drugs, including delamanid (Pa), moxifloxacin (M), pyrazinamide (Z), and polylactic acid-co-glycolic acid mixed in a ratio of 3:12:45:140, was incorporated onto the surface of the ceramic artificial bone. Consequently, a high-strength ceramic artificial bone, loaded with anti-tuberculosis drugs, was successfully obtained. The physical characteristics of the drug-loaded artificial bone were assessed using an electronic universal testing machine and scanning electron microscopy. The osteogenic performance of the artificial bone was evaluated through rat bone marrow mesenchymal stem cell (rBMSCs) co-culture experiment, cell counting kit-8 (CCK-8) cell proliferation assay, alkaline phosphatase staining, and alizarin red staining. The drug-loaded ceramic artificial bone exhibited favorable physical characteristics, void interconnection, a porosity of 30.6% ± 0.7%, and a compressive strength of 17.65 ± 0.46 MPa. The rBMSCs co-culture experiment and CCK-8 cell proliferation experiment demonstrated excellent cell compatibility, while alkaline phosphatase and alizarin red staining indicated good in vitro osteogenic performance. In summary, the high-strength ceramic artificial bone loaded with the anti-tuberculosis drug PaMZ exhibited a favorable morphological structure and compressive strength. In addition, it demonstrated good biocompatibility and osteogenic properties.

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Coupling Azo Dye Methods for Histochemical Demonstration of Alkaline Phosphatase

Cited by 35 publications

References 2 publications

ZnO nanoparticles augment ALT, AST, ALP and LDH expressions in C2C12 cells

ZnO nanoparticles augment ALT, AST, ALP and LDH expressions in C2C12 cells

Histochemical Localization of Alkaline Phosphatase in the Retina

Preparation and in vitro osteogenic evaluation of high-strength ceramic artificial bone loaded with anti-tuberculosis drug PaMZ

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