Coupling between Cl- absorption and HCO3- secretion in turtle urinary bladder

Leslie, BR; Schwartz, JH; Steinmetz, P. R.

doi:10.1152/ajplegacy.1973.225.3.610

Cited by 80 publications

(40 citation statements)

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“…In several bladders, deoxygenation inhibited as much as 80% of the total HCOs-flow, a fraction approaching the electroneutral component which was 82% in the study by Leslie et al (4). Although the average inhibition by deoxygenation was somewhat less, about 60% in our study, it should be stated that deoxygenation was not necessarily complete.…”

Section: Discussioncontrasting

confidence: 50%

“…The three pump systems are not coupled directly to each other. Ouabain, which inhibits Na+ transport, has no direct effect on either H+ secretion or Cl-HCO3 exchange transport (4). The present study suggests that the anion exchange pump depends largely on aerobic energy.…”

Section: Discussioncontrasting

confidence: 42%

“…Since the diffusional component is small, HCO3-secretion refers mainly to the electroneutral flow which is coupled to Cl-absorption (4 (4). Unidirectional 'Cl-fluxes were performed as previously described (5).…”

Section: Introductionmentioning

confidence: 99%

“…Since measurement of the S to M HCOi-flux requires the absence of net H+ secretion, the M solution for each bladder was acidified to the pH at which net-acid secretion was zero (pH range 4.5-52), while the pH of S was adjusted with NaOH to 8.5 in the absence of exogenous C02 and HCOs (4 The production of lactate by the bladders was not measured in the present study in which the deoxygenation periods were short. To provide an estimate of changes in lactate production in our system, we include data on the rate of lactate production before and after deoxygenation in a series of different experiments (Schwartz and Steinmetz, unpublished observations) in which the gas phase was changed in identical manner and lactate accumulation was measured in the bathing solutions over 1-h periods.…”

Section: Introductionmentioning

confidence: 99%

“…All experiments were carried out at room temperature and as in previous studies (4,5), all rates are expressed as micromoles per hour per 8 cm2 of membrane area + the SE of the mean. This membrane area represented a mean dry weight of 14.7+0.6 mg per 8 cm2 in a previous study carried out with the same technique (4).…”

Section: Introductionmentioning

confidence: 99%

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Energy dependence of urinary bicarbonate secretion in turtle bladder.

Oliver¹,

Himmelstein²,

Steinmetz³

1975

J. Clin. Invest.

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A B S T R A C T Addition of HCOi-to the serosal side (S) of the isolated turtle bladder results in a HCO0-flow from S to the mucosal side (M) which markedly reduces the net rate of acid secretion. To characterize the driving forces for this downhill HCO3-flow, the effects of metabolic inhibitors and substrates were examined. In short-circuited bladders with the M pH lowered to the point of zero net H+ secretion, the rate of HCO-entry into M in response to a 20-mM HCO3-gradient was measured by pH stat titration. Deoxygenation reduced the HC03-flux from 1.24±0.1 gM/h/8 cm2 (SEM) to 0.50±0.1 tM/h with glucose (2 X 10' M) and from 1.32±0.1 to 0.47±0.1 iAM/h without glucose.A similar reduction (61%) was observed in the presence of 1% C02. Dinitrophenol (10' M), cyanide (10' M), and deoxyglucose (10-' M) inhibited the HCO3-flux by 39%, 37%, and 38%, respectively. The combination of any of these inhibitors with N2 caused the same inhibition as Ns alone. In bladders depleted of substrate, pyruvate (5 X 10-' M) increased the HCOs-flux from 0.36 ±0.05 to 0.58±0.01 ,M/h (P < 0.005); the increment was abolished by deoxygenation.The results indicate that the bulk of the downhill HCOi-flow in this system is dependent on metabolic energy derived primarily from oxidative sources, and that this energy-dependent flow approximates the electroneutral component of HCO3-secretion that is coupled to Cl-absorption.

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Section: Discussioncontrasting

confidence: 50%

Section: Discussioncontrasting

confidence: 42%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Energy dependence of urinary bicarbonate secretion in turtle bladder.

Oliver¹,

Himmelstein²,

Steinmetz³

1975

J. Clin. Invest.

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Cellular Mechanisms of H⁺and HCO₃⁻transport in tight urinary epithelia

Al‐Awqati

Beauwens

1992

Comprehensive Physiology

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The sections in this article are: Transepithelial Proton and Bicarbonate Transport Intercalated Cells Proton Secretion Bicarbonate Secretion Molecules Involved in Acid‐Base Transport Proton‐Translocating ATPases Cl − − Exchanger Chloride Channel Carbonic Anhydrase Regulation of Proton Secretion Transmembrane Electrochemical Gradient Cell pH Effect of CO 2 and Role of Exocytosis Role of Endocytosis Aldosterone Extracellular Acid‐Base Changes Cyclic AMP Regulation of Bicarbonate Secretion Exfracellular Acid‐Base Changes c AMP Cell Calcium and the Calcium Ionophore A23187 Epithelial Polarity of Proton and Bicarbonate Transport Biogenesis of Epithelial Polarity Plasticity of Epithelial Polarity in the Intercalated Cell Other Examples of Reversible Polarity Potential Mechanisms for Plasticity in the Polarity of the Intercalated Cell Developmental Biology of the Intercalated Cell

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Renal Acidification: Cellular Mechanisms of Tubular Transport and Regulation

Alpern

Rector

1992

Comprehensive Physiology

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The sections in this article are: Newer Techniques Carbonic Anhydrase Mechanisms of Proximal Tubular Acidification Apical Membrane Basolateral Membrane Leak Pathways Carbonic Anhydrase Summary Mechanisms of Loop of Henle Acidification Mechanisms of Distal Nephron Acidification Mechanism of Proton Secretion (Bicarbonate Absorption) Mechanism of Bicarbonate Secretion Leak Pathways Cells Mediating H + / Transport Carbonic Anhydrase Mechanisms of Regulation of Acidification Proximal Tubule Distal Tubule

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Coupling between Cl- absorption and HCO3- secretion in turtle urinary bladder

Cited by 80 publications

References 0 publications

Energy dependence of urinary bicarbonate secretion in turtle bladder.

Energy dependence of urinary bicarbonate secretion in turtle bladder.

Cellular Mechanisms of H⁺and HCO₃⁻transport in tight urinary epithelia

Renal Acidification: Cellular Mechanisms of Tubular Transport and Regulation

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Coupling between Cl- absorption and HCO3- secretion in turtle urinary bladder

Cited by 80 publications

References 0 publications

Energy dependence of urinary bicarbonate secretion in turtle bladder.

Energy dependence of urinary bicarbonate secretion in turtle bladder.

Cellular Mechanisms of H+and HCO3−transport in tight urinary epithelia

Renal Acidification: Cellular Mechanisms of Tubular Transport and Regulation

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Cellular Mechanisms of H⁺and HCO₃⁻transport in tight urinary epithelia