“…Combining multiple compensation voltages (CVs) within a single run or between runs improves whole proteome coverage in cell lysates. , For example, Pfammatter et al observed a 30% gain in unique peptide identification using FAIMS compared with non-FAIMS analysis of HEK 293 cells . Other studies using HeLa protein digest such as Johnson KR et al, Greguš M et al, and Cong Y et al concurrently showed the addition of FAIMS had significantly increased protein and peptide identifications than without FAIMS. − In a few recent studies working with various tissue samples, including brain autopsies, tumor biopsies, and paraffin embedded tissues (lymph node, lung, and prostate), the addition of FAIMS also showed an enhanced detection of nonredundant proteoforms, increased proteome sensitivity, or substantially reduced sample handling. As the cardiac proteome consists of large dynamic range, it remains unclear whether FAIMS can detect peptides and the less abundant proteins from the cardiac proteome that is comparable with previous established fractionation methods.…”