Coupling <i>in vitro</i> food digestion with <i>in vitro</i> epithelial absorption; recommendations for biocompatibility

Kondrashina, Alina; Arranz, Elena; Cilla, Antonio; Faria, Miguel A.; Santos-Hernández, Marta; Miralles, Beatriz; Hashemi, Negin; Rasmussen, Martin Krøyer; Young, Jette F.; Barberá, Reyes; Mamone, Gianfranco; Tomás‐Cobos, Lidia; Bastiaan‐Net, Shanna; Corredig, Milena; Giblin, Linda

doi:10.1080/10408398.2023.2214628

Cited by 25 publications

(21 citation statements)

References 128 publications

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“…Thus, the heterogeneity in transporter activity of several Caco-2 clones was ascribed to differences in transporter expression, as shown for PepT1 and MDR1, and this was determined by the culture conditions . Moreover the biocompatibility of complex samples such as food digesta with intestinal cell models needs particular attention . This has led this group of researchers to work on the standardization of an in vitro model to be applied to such substrates.…”

Section: The Need To Standardize In Vitro Modelsmentioning

confidence: 99%

“…27 Moreover the biocompatibility of complex samples such as food digesta with intestinal cell models needs particular attention. 61 This has led this group of researchers to work on the standardization of an in vitro model to be applied to such substrates. First, it is necessary to have a shared procedure for the manipulation of the intestinal cell lines as well as for the preparation of the cell monolayers ready for absorption experiments.…”

Section: The Need To Standardize In Vitro Modelsmentioning

confidence: 99%

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A Shared Perspective on in Vitro and in Vivo Models to Assay Intestinal Transepithelial Transport of Food Compounds

Hevia,

Ruas-Madiedo,

Faria

et al. 2023

J. Agric. Food Chem.

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Assessing nutrient bioavailability is complex, as the process involves multiple digestion steps, several cellular environments, and regulatory-metabolic mechanisms. Several in vitro models of different physiological relevance are used to study nutrient absorption, providing significant challenges in data evaluation. However, such in vitro models are needed for mechanistic studies as well as to screen for biological functionality of the food structures designed. This collaborative work aims to put into perspective the wide-range of models to assay the permeability of food compounds considering the particular nature of the different molecules, and, where possible, in vivo data are provided for comparison.

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Section: The Need To Standardize In Vitro Modelsmentioning

confidence: 99%

Section: The Need To Standardize In Vitro Modelsmentioning

confidence: 99%

A Shared Perspective on in Vitro and in Vivo Models to Assay Intestinal Transepithelial Transport of Food Compounds

Hevia,

Ruas-Madiedo,

Faria

et al. 2023

J. Agric. Food Chem.

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“…10 To date, several studies investigated the effect of single food bioactive compounds, [11][12][13] food extracts, 14,15 or food digests in cell cultures. 16,17 Studies aimed at evaluating the nutritional and healthful properties of specific fractions/compounds in CBW are systematically reviewed here, to highlight any connection between their molecular properties and their effects on the investigated system. This may allow a somehow deeper insight into the molecular bases underpinning the potential advantages of using CBW as a key ingredient in functional foods.…”

Section: Introductionmentioning

confidence: 99%

Cell culture models for assessing the effects of bioactive compounds in common buckwheat (Fagopyrum esculentum): a systematic review

Borgonovi,

Iametti,

Speranza

et al. 2024

Food Funct.

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“…In addition, after digestion, bioaccessible compounds with beneficial health effects as well as unwanted compounds can be obtained [ 22 ]. In this sense, the possible toxic effect of the digestion products of a designed food can be evaluated using cellular models.…”

Section: Introductionmentioning

confidence: 99%

“…In this sense, the possible toxic effect of the digestion products of a designed food can be evaluated using cellular models. Among the cell lines, the human colon adenocarcinoma line (Caco-2) stands out because it has the ability to differentiate into enterocytes and simulate the intestinal epithelium, becoming a widely used tool to study the absorption and transport of analytes of interest, as well as their cytotoxicity [ 22 , 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

An Apple and Acáchul Berry Snack Rich in Bioaccessible Antioxidants and Folic Acid: A Healthy Alternative for Prenatal Diets

Corfield,

Allievi,

Rivero

et al. 2024

Foods

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A fruit leather (apple and acáchul berry) oriented toward women of reproductive age was developed. The snack was supplemented with an ingredient composed of folic acid (FA) and whey proteins (WPI) to ensure the required vitamin intake to prevent fetal neural tube defects. In order to generate a low-calorie snack, alternative sweeteners were used (stevia and maltitol). The fruit leather composition was determined. Also, an in vitro digestion process was carried out to evaluate the bioaccessibility of compounds with antioxidant capacity (AC), total polyphenols (TPCs), total monomeric anthocyanins (ACY), and FA. The quantification of FA was conducted by a microbiological method and by HPLC. The leather contained carbohydrates (70%) and antioxidant compounds, mainly from fruits. Bioaccessibility was high for AC (50%) and TPCs (90%), and low for ACY (17%). Regarding FA, bioaccessibility was higher for WPI-FA (50%) than for FA alone (37%), suggesting that WPI effectively protected the vitamin from processing and digestion. Furthermore, the product was shown to be non-cytotoxic in a Caco-2 cell model. The developed snack is an interesting option due to its low energy intake, no added sugar, and high content of bioactive compounds. Also, the supplementation with WPI-FA improved the conservation and bioaccessibility of FA.

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Coupling in vitro food digestion with in vitro epithelial absorption; recommendations for biocompatibility

Cited by 25 publications

References 128 publications

A Shared Perspective on in Vitro and in Vivo Models to Assay Intestinal Transepithelial Transport of Food Compounds

A Shared Perspective on in Vitro and in Vivo Models to Assay Intestinal Transepithelial Transport of Food Compounds

Cell culture models for assessing the effects of bioactive compounds in common buckwheat (Fagopyrum esculentum): a systematic review

An Apple and Acáchul Berry Snack Rich in Bioaccessible Antioxidants and Folic Acid: A Healthy Alternative for Prenatal Diets

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