Coal seam pre-drainage technology is widely used to prevent and control gas in China’s high-gas coal seams, and upward drilling is a safe and reliable technology. Using the Luling Coal Mine as the study location, according to the actual situation of the site, a layer-through drilling process was proposed. Relying on a cross-layer extraction borehole, coal seam water injection efficiency extraction and high-efficiency water injection dust prevention can be performed in the later stage of borehole extraction, enabling one-hole multi-purpose and “gas-powder” integrated prevention and control. In order to study the “stress-seepage” coupling characteristics of the coal around the borehole, a numerical simulation of the coal seam water injection process was conducted. Based on the simulation results, a field test of water injection in coal seam upward drilling was performed. The field monitoring data show that the water injection and water content of the coal seam were increased by about 3.07 m3 and 0.69%, respectively, compared with the traditional water injection process. The water injection effect is remarkable, which is of great significance to ensure the safe and efficient production of the mine.