Coupling Mechanism of Electromagnetic Field and Thermal Stress on Drosophila melanogaster

Zhang, Ziyan; Zhang, Jing; Yang, Cao; Lian, Huiyong; Yu, Hui; Huang, Xiaomei; Cai, Peng

doi:10.1371/journal.pone.0162675

Cited by 13 publications

(7 citation statements)

References 50 publications

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“…calculated to be equal to 0.15 W/kg, according to the formula SAR D (s¢E_RMS^2)/r W/kg. A recent publication by Zhang et al 64 concluded that thermal stress weakened the physiologic function and promoted the HSR (Heat Shock Response) and OS (Oxidative Stress) of flies. ELF-EMF aggravated damages and enhanced thermal stress-induced HSP and OS responses.…”

Section: Exposure Conditionsmentioning

confidence: 99%

Mobile-phone radiation-induced perturbation of gene-expression profiling, redox equilibrium and sporadic-apoptosis control in the ovary ofDrosophila melanogaster

Manta

Papadopoulou

Polyzos

et al. 2017

Fly

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The daily use by people of wireless communication devices has increased exponentially in the last decade, begetting concerns regarding its potential health hazards. Drosophila melanogaster four days-old adult female flies were exposed for 30 min to radiation emitted by a commercial mobile phone at a SAR of 0.15 W/kg and a SAE of 270 J/kg. ROS levels and apoptotic follicles were assayed in parallel with a genome-wide microarrays analysis. ROS cellular contents were found to increase by 1.6-fold (x), immediately after the end of exposure, in follicles of pre-choriogenic stages (germarium - stage 10), while sporadically generated apoptotic follicles (germarium 2b and stages 7-9) presented with an averaged 2x upregulation in their sub-population mass, 4 h after fly's irradiation with mobile device. Microarray analysis revealed 168 genes being differentially expressed, 2 h post-exposure, in response to radiofrequency (RF) electromagnetic field-radiation exposure (≥1.25x, P < 0.05) and associated with multiple and critical biological processes, such as basic metabolism and cellular subroutines related to stress response and apoptotic death. Exposure of adult flies to mobile-phone radiation for 30 min has an immediate impact on ROS production in animal's ovary, which seems to cause a global, systemic and non-targeted transcriptional reprogramming of gene expression, 2 h post-exposure, being finally followed by induction of apoptosis 4 h after the end of exposure. Conclusively, this unique type of pulsed radiation, mainly being derived from daily used mobile phones, seems capable of mobilizing critical cytopathic mechanisms, and altering fundamental genetic programs and networks in D. melanogaster.

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Section: Exposure Conditionsmentioning

confidence: 99%

Mobile-phone radiation-induced perturbation of gene-expression profiling, redox equilibrium and sporadic-apoptosis control in the ovary ofDrosophila melanogaster

Manta

Papadopoulou

Polyzos

et al. 2017

Fly

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“…The increase in HSP70 mRNA levels after heat and MF exposure was detected also in animal models, such as in transgenic Caenorhabditis elegans nematodes and in Drosophila malanogaster . In C. elegans , 1 h concurrent exposure to 50 and 100 µT under 28 and 29°C thermal stress was carried out ( 56 ) while, in Drosophila , 2 and 24 h concurrent exposures were carried out at 35°C ( 57 ). The potentiation of heat-induced HSP16 ( 56 ) and HSP22 and HSP26 ( 57 ) expression by concurrent MF was detected.…”

Section: Combined Exposure To Elf-mf and Heatmentioning

confidence: 99%

Cellular Response to ELF-MF and Heat: Evidence for a Common Involvement of Heat Shock Proteins?

Zeni

Simkó²,

Scarfì

et al. 2017

Front. Public Health

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It has been shown that magnetic fields in the extremely low frequency range (ELF-MF) can act as a stressor in various in vivo or in vitro systems, at flux density levels below those inducing excitation of nerve and muscle cells, which are setting the limits used by most generally accepted exposure guidelines, such as the ones published by the International Commission on Non-Ionizing Radiation Protection. In response to a variety of physiological and environmental factors, including heat, cells activate an ancient signaling pathway leading to the transient expression of heat shock proteins (HSPs), which exhibit sophisticated protection mechanisms. A number of studies suggest that also ELF-MF exposure can activate the cellular stress response and cause increased HSPs expression, both on the mRNA and the protein levels. In this review, we provide some of the presently available data on cellular responses, especially regarding HSP expression, due to single and combined exposure to ELF-MF and heat, with the aim to compare the induced effects and to detect possible common modes of action. Some evidence suggest that MF and heat can act as costressors inducing a kind of thermotolerance in cell cultures and in organisms. The MF exposure might produce a potentiated or synergistic biological response such as an increase in HSPs expression, in combination with a well-defined stress, and in turn exert beneficial effects during certain circumstances.

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“…The increase in this lipid peroxidation marker was observed after 24-h exposure and remained elevated until 7-day exposure. Zhang et al ( 2016 ) have shown that effect of EMF on MDA levels is sex-dependent. They observed decline in MDA level in male, but not in female Drosophila exposed to EMF (50 Hz, 3 mT) for 12 h. However, in our experiments, the intensity and duration of EMF exposure was higher, what could act as a marked stressor.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, significant changes in levels of antioxidants, such as glutathione, superoxide dismutase or catalase were observed (Kivrak et al 2017 ). Zhang et al ( 2016 ) demonstrated that thermal stress (35 °C) and EMF exposure (50 Hz, 3 mT) elicit a synergistic effect, strengthening the negative effect of EMF on lifespan, locomotion and oxidative stress in Drosophila melanogaster .…”

Section: Introductionmentioning

confidence: 99%

Electromagnetic field exposure (50 Hz) impairs response to noxious heat in American cockroach

et al. 2018

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Exposure to electromagnetic field (EMF) induces physiological changes in organism that are observed at different levels—from biochemical processes to behavior. In this study, we evaluated the effect of EMF exposure (50 Hz, 7 mT) on cockroach’s response to noxious heat, measured as the latency to escape from high ambient temperature. We also measured the levels of lipid peroxidation and glutathione content as markers of oxidative balance in cockroaches exposed to EMF. Our results showed that exposure to EMF for 24, 72 h and 7 days significantly increases the latency to escape from noxious heat. Malondialdehyde (MDA) levels increased significantly after 24-h EMF exposure and remained elevated up to 7 days of exposure. Glutathione levels significantly declined in cockroaches exposed to EMF for 7 days. These results demonstrate that EMF exposure is a considerable stress factor that affects oxidative state and heat perception in American cockroach.

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Coupling Mechanism of Electromagnetic Field and Thermal Stress on Drosophila melanogaster

Cited by 13 publications

References 50 publications

Mobile-phone radiation-induced perturbation of gene-expression profiling, redox equilibrium and sporadic-apoptosis control in the ovary ofDrosophila melanogaster

Mobile-phone radiation-induced perturbation of gene-expression profiling, redox equilibrium and sporadic-apoptosis control in the ovary ofDrosophila melanogaster

Cellular Response to ELF-MF and Heat: Evidence for a Common Involvement of Heat Shock Proteins?

Electromagnetic field exposure (50 Hz) impairs response to noxious heat in American cockroach

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