Coupling of a DNA piezoelectric biosensor and polymerase chain reaction to detect apolipoprotein E polymorphisms

Tombelli, Sara; Mascini, Marco; Braccini, Leonardo; M, Anichini; Turner, Anthony P. F.

doi:10.1016/s0956-5663(00)00092-0

Cited by 63 publications

(23 citation statements)

References 27 publications

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“…By use of the PPF technique, which achieved extremely thin and homogenous coating, antibodies were covalently immobilized on the surface of the QCM chip by means of an amino group incorporated in the PPF. Other applications of the QCM device are cited in this article [76,77,84,85,86,97,153,160,161,162,163,164,165,166,167,168].…”

Section: Quartz Crystal Microbalance (Qcm) Sensorsmentioning

confidence: 99%

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Current research activity in biosensors

Nakamura

Karube

2003

Analytical and Bioanalytical Chemistry

261

129

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Biosensors consist of a molecular recognition element and a transducer. Since the first biosensor was developed by Updike and Hicks many biosensors and their associated techniques have been studied and developed. In this review current research activity on the fundamentals and applications of biosensors is summarized. In discussion of the former, molecular recognition elements, techniques and tools for biosensor construction, and basic biosensor devices are introduced. Coverage of the latter includes description of biosensors for environmental, food, and clinical fields. Chemical sensors developed in our laboratory for environmental monitoring are also described. This review mainly summarizes work performed by our group and by our colleagues, and refers to the main review articles summarizing each field of biosensor research in recent years.

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Section: Quartz Crystal Microbalance (Qcm) Sensorsmentioning

confidence: 99%

“…A DNA piezoelectric biosensor and PCR have been coupled to detect apolipoprotein E polymorphisms as a point mutation in a human gene [164]. The device was able to distinguish among the different synthetic oligonucleotides such as complementary, non-complementary, and mismatched DNA.…”

Section: Sensors For Other Body Constituentsmentioning

confidence: 99%

Current research activity in biosensors

Nakamura

Karube

2003

Analytical and Bioanalytical Chemistry

261

129

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“…Genetic defects were identified with a TSM biomedical sensor by measuring a single mismatch in a 15mer single-strand target of the p53 suppresser gene [127]. Other work reports a successful study on denaturation of DNA [128], the detection of DNA polymorphisms [129] and the real-time monitoring of enzymatic cleavage of nucleic acids [130]. In comparison to traditional DNA analysis methods [123], acoustic sensors offer a rapid and marker-free detection of nucleic acid hybridization.…”

Section: ) Acoustic Waves Piezoelectric Transducers and Acoustic Sementioning

confidence: 99%

Emerging biomedical sensing technologies and their applications

2003

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Abstract-Recent progress in biomedical sensing technologies has resulted in the development of several novel sensor products and new applications. Modern biomedical sensors developed with advanced microfabrication and signal processing techniques are becoming inexpensive, accurate, and reliable. A broad range of sensing mechanisms has significantly increased the number of possible target measurands that can be detected. The miniaturization of classical "bulky" measurement techniques has led to the realization of complex analytical systems, including such sensors as the BioChemLab-on-a-Chip. This rapid progress in miniature devices and instrumentation development will significantly impact the practice of medical care as well as future advances in the biomedical industry. Currently, electrochemical, optical, and acoustic wave sensing technologies have emerged as some of the most promising biomedical sensor technologies. In this paper, important features of these technologies, along with new developments and some of the applications, are presented.

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“…Then, streptavidin-biotin binding is exploited to attach and immobilize the aptamer (ala DNA, as discussed earlier). Such an approach was established by Tombelli and coworkers [116,148,149] for the detection of HIV-1 Tat (trans-activator of transcription) protein.…”

Section: Aptamersmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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Viruses are pathogenic microorganisms that can inhabit and replicate in human bodies causing a number of widespread infectious diseases such as influenza, gastroenteritis, hepatitis, meningitis, pneumonia, acquired immune deficiency syndrome (AIDS) etc. A majority of these viral diseases are contagious and can spread from infected to healthy human beings. The most important step in the treatment of these contagious diseases and to prevent their unwanted spread is to timely detect the disease-causing viruses. Gravimetric viral diagnostics based on quartz crystal microbalance (QCM) transducers and natural or synthetic receptors are miniaturized sensing platforms that can selectively recognize and quantify harmful virus species. Herein, a review of the label-free QCM virus sensors for clinical diagnostics and point of care (POC) applications is presented with major emphasis on the nature and performance of different receptors ranging from the natural or synthetic antibodies to selective macromolecular materials such as DNA and aptamers. A performance comparison of different receptors is provided and their limitations are discussed.

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Coupling of a DNA piezoelectric biosensor and polymerase chain reaction to detect apolipoprotein E polymorphisms

Cited by 63 publications

References 27 publications

Current research activity in biosensors

Current research activity in biosensors

Emerging biomedical sensing technologies and their applications

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

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