Coupling of CE‐MS for protein and peptide analysis

Mikšı́k, Ivan

doi:10.1002/jssc.201800817

Cited by 52 publications

(31 citation statements)

References 79 publications

(107 reference statements)

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“…Both, IEF and zone electrophoresis in CE format (CIEF and CZE) have been successfully combined with MS (CE–MS) for peptide and protein analysis . Technological advancements in instrumentation, coupling to mass spectrometer and improvements in separation performance have accompanied this development . Even though nanoLC–MS is still the most commonly used analysis platform for advanced proteomics , several studies have demonstrated that combination of nanoLC–MS and CE–MS methods increase the number of identified proteins as they are orthogonal and provide complementary results .…”

Section: Introductionmentioning

confidence: 99%

Sodium dodecyl sulfate free gel electrophoresis/electroelution sorting for peptide fractionation

Ramos

González

Sosa‐Acosta

et al. 2019

J of Separation Science

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Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide gel electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.

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Section: Introductionmentioning

confidence: 99%

Sodium dodecyl sulfate free gel electrophoresis/electroelution sorting for peptide fractionation

Ramos

González

Sosa‐Acosta

et al. 2019

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…It is well suited to highly charged polar metabolites such as organics acids, nucleotides, peptides and their conjugates. It is coupled to MS instruments using electrospray ionisation ( 27 ) . For high-throughput techniques where it is desirable to have low run time per sample direct infusion MS is often employed.…”

Section: Metabolomics: Role In Biomarker Discoverymentioning

confidence: 99%

“…Other 80 databases include MyCompoundID, a library of 8,021 endogenous human metabolites with 10, 583,901 predicted products of these metabolites nucleotides, peptides and their conjugates. It is coupled to MS instruments using electrospray 114 ionisation (ESI) (27) . For high through-put techniques where it is desirable to have low run 115 time per sample direct infusion mass spectrometry (DIMS) is often employed.…”

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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Role of metabolomics in identification of biomarkers related to food intake

2019

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24Dietary assessment methods including food-frequency questionnaires and food diaries are 25 associated with many measurement errors including energy under-reporting and incorrect 26 estimation of portion sizes. Such errors can lead to inconsistent results especially when 27 investigating the relationship between food intake and disease causation. To improve the 28 classification of a person's dietary intake and therefore clarify proposed links between diet 29 and disease, reliable and accurate dietary assessment methods are essential. Dietary 30 biomarkers have emerged as a complimentary approach to the traditional methods and in 31 recent years, metabolomics has developed as a key technology for the identification of new 32 dietary biomarkers. The objective of this review is to give an overview of the approaches 33 used for the identification of biomarkers and potential use of the biomarkers. 34Over the years a number of strategies have emerged for the discovery of dietary biomarkers 35 including acute and medium term interventions and cross-sectional/cohort study approaches. 36Examples of the different approaches will be presented. Concomitant with the focus on single 37 biomarkers of specific foods there is an interest in development of biomarker signatures for 38 the identification of dietary patterns. In the present review we present an overview of the 39 techniques used in food intake biomarker discover and the experimental approaches used for 40 biomarker discovery and challenges faced in the field. While significant progress has been 41 achieved in the field of dietary biomarkers in recent years a number of challenges remain. 42Addressing these challenges will be key to ensure success in implementing use of dietary 43 biomarkers. 44 45 48 research. One of the areas with great expectations is the field of dietary biomarkers or food 49 intake biomarkers. The interest in these biomarkers stems from the need for objective 50 measures of dietary intake. The traditional methods such as food frequency questionnaires 51 (FFQs), 24 h recalls and food diaries are all associated with a number of well-defined 52 limitations including under-reporting, recall errors and difficulty in assessment of portion 53 sizes (1)(2)(3) . Currently dietary biomarkers include 24h urinary sodium, nitrogen and 54 sucrose/fructose for estimation of salt, protein and sugar intake (4)(5)(6)(7) . In recent years, the 55 concept of biomarkers reflecting specific food intake has emerged. To date a number of 56 putative biomarkers exist for the intake of a range of foods including but not limited to red 57 meat, coffee, nuts, wine, vegetables, legumes, citrus fruit, tea, sugar sweetened beverages (7-58 11) . While some confusion exists in the literature over classification of biomarkers into 59 recovery or concentration biomarkers we prefer to use the newly defined flexible 60 classification scheme for biomarkers related to food intake (12) . Food intake biomarkers are 61 single metabolites, or a combination of metabolites, reflecting ...

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“…Son aşamada ise MS yöntemi ile bu proteinlerin kütleleri saptanmakta ve kesin ve doğru olarak proteinlerin tek tek tanımlanması gerçekleştirilmektedir. 3,14 Örnek içerisinde az miktarda bulunan ama belirteç (marker) olabilecek proteinlerin ayrımı için çok hassas bir çalışma gerekmektedir. Proteomik teknolojisine bu proteinlerin tanımlanması, hastalığın gelişimi ile ilgisi ve bu verilerin bilgi bankalarında toplanarak değerlendirilmesi ve normal biyolojik süreçler ile hastalıkların altında yatan mekanizmaların tanımlanması amaçlanmaktadır.…”

Section: Proteomi̇k Anali̇zlerunclassified