Coupling of large haptens to proteins and cell surfaces: Preparation of stable, optimally sensitized erhythrocytes for hapten-specific, hemolytic plaque assays

Inman, John K.; Merchant, Bruce; Claflin, Latham; Tacey, Sue Ellen

doi:10.1016/0019-2791(73)90005-0

Cited by 84 publications

(32 citation statements)

References 22 publications

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“…OVA and GPA were also conjugated with DNP-,B-alanyl-glycyl-glycyl-azide (DAG) by the method of Inman et al . (10) . KLH and OVA were conjugated with 1-fluoro-2-nitrobenzene, 1-fluoro-4-nitrobenzene, picryl chloride (all from Eastman Organic Chemicals Div., Eastman Kodak Co .…”

Section: Table I Composition Of Synthetic Antigens Used In These Studiesmentioning

confidence: 99%

The specificity of cellular immune responses in guinea pigs. I. T cells specific for 2,4-dinitrophenyl-o-tyrosyl residues.

Janeway¹,

Cohen²,

Ben-Sasson³

et al. 1975

The Journal of Experimental Medicine

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Responses of thymus-derived (T) lymphocytes to hapten-protein conjugates are very largely specific for carrier-and conjugate-specific determinants ; such responses rarely show the hapten specificity which predominates in the responses of thymus-independent bone marrow-derived (B) lymphocytes to similar antigens (1) . These findings, and similar observations in other antigen systems, have led to the suggestion that T and B cells have distinctive libraries of specific receptors, or perhaps very different requirements for activation (2-4) . However, it has not in fact been possible to precisely analyze T-cell receptor specificity, except in a few cases, because simple, well defined chemical determinants can rarely be employed for this purpose . Nevertheless, T-cell responses apparently specific for hapten occur under certain circumstances, as described by Leskowitz et al . (5) in the p-azo-benzenearsonate system, and in recent extensions of these studies by Alkan et al . (6) . In addition, Benacerraf and Gell (7) reported that immunization of guinea pigs with 2,4,6-trinitrophenylated Mycobacteria induced a state of delayed hypersensitivity (DH)' which could be elicited by 2,4,6-trinitrophenyl (TNP) coupled to several proteins, and these results have recently been confirmed by Trefts et al .' In the present experiments, the specificity of T lymphocytes has been explored by immunizing inbred guinea pigs to 2,4-dinitrophenyl (DNP) Mycobacteria . We confirm that DH responses to a variety of DNP proteins can be obtained in such animals .and in addition we show that contact reactivity to 1-fluoro-2,4-dinitrobenzene (DNFB) and in vitro DNA-synthetic and migration inhibition responses are obtained . However, several lines of evidence clearly demonstrate *Present address :

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Section: Table I Composition Of Synthetic Antigens Used In These Studiesmentioning

confidence: 99%

The specificity of cellular immune responses in guinea pigs. I. T cells specific for 2,4-dinitrophenyl-o-tyrosyl residues.

Janeway¹,

Cohen²,

Ben-Sasson³

et al. 1975

The Journal of Experimental Medicine

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“…All other compounds were covalently bound to cells using haptens in the form of an acyl azide as previously described for NIP-AGG ( 11,14,15). These compounds included: N-(3-nitro-4-hydroxy-5-iodophenylacetyl)-B-alanylglycylglycyl (NIP-AGG) azide; N-(3-nitro-4-hydroxyphenylacetyl)-flalanylglycylglycyl (NP-AGG) azide; N-(3-nitro-4-hydroxy-5-iodophenylacetyl) (NIP) azide; and N-(2,4,6-trinitrophenyl)-fl-alanyglycylglycyl (TNP-AGG) azide.…”

mentioning

confidence: 99%

Cell-mediated lympholysis to H-2-matched target cells modified with a series of nitrophenyl compounds.

Rehn¹,

Inman²,

Shearer³

1976

The Journal of Experimental Medicine

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The specificity of C57BL/10 cytotoxic effector cells generated by in vitro sensitization with autologous spleen cells modified with a series of related nitrophenyl compounds was investigated. The failure of trinitrophenyl (TNP)-sensitized effector cells to lyse TNP-beta-alanylglycylglycyl(AGG)-modified target cells is presented as evidence contradicting the intimacy or dual receptor model or T-cell recognition in its simplest form. Data are also shown indicating that sensitization with N-(3-nitro-4-hydroxy-5-iodophenylacetyl)-AGG-modified stimulating cells generates noncross-reacting clones of cytotoxic effector cells.

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“…The number of direct plaque-forming cells (PFC) in spleen cell suspensions was determined by a modification of the Jeme hemolytic plaque assay as described by Merchant and Inman (16). SRBC or DNP-SRBC, prepared according to the procedure of Inman et al (17) were used as indicator erythrocytes.…”

Section: Methodsmentioning

confidence: 99%

Immunomodulating properties of two synthetic adjuvants: Dependence upon type of antigen, dose, and time of administration

Hilgers

Snippe

Jansze

et al. 1984

Cellular Immunology

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The effects of two synthetic adjuvants on the antibody response to sheep red blood cells (SRBC) as a thymus dependent (TD) antigen and to dinitropheny&s-Ficoll as a thymus-independent (TI-1) antigen were investigated in mice. Both dimethyldioctadecylammonium bromide (DDA) and dextran sulfate (DXS) augmented the humoral response to SRBC but not to dinitrophenylJgFicoll if injected simultaneously with antigen. Dose-response curves of both antigen and adjuvant revealed that DXS compared to DDA is a more effective adjuvant for the induction of a humoral response to SRBC. Intraperitoneal injection of DDA or DXS evoked a sequence of distinct immune responsive states in mice, measured by the capacity to develop an anti-SRBC response. A short immune-potentiating period (~6 hr) is followed by a suppressive, second immunepotentiating state. The immune suppressive state lasted for a period of about 8 days and was restricted to TD-antigens. Suppression could be totally overridden by injection of DDA or DXS simultaneously with antigen, suggesting that the suppressive state was reversible. The kinetics of the observed alteration of the immune response by DDA and DXS were very similar. It is concluded that differences in the modulation of the immune response by DDA and DXS are limited to the initial state. Long-term effects like the induction of a succession of distinct immune responsive states, are more or less similar for both adjuvants. Possible mechanisms by which these immunomodulators interfere with the immune system are discussed.

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Coupling of large haptens to proteins and cell surfaces: Preparation of stable, optimally sensitized erhythrocytes for hapten-specific, hemolytic plaque assays

Cited by 84 publications

References 22 publications

The specificity of cellular immune responses in guinea pigs. I. T cells specific for 2,4-dinitrophenyl-o-tyrosyl residues.

The specificity of cellular immune responses in guinea pigs. I. T cells specific for 2,4-dinitrophenyl-o-tyrosyl residues.

Cell-mediated lympholysis to H-2-matched target cells modified with a series of nitrophenyl compounds.

Immunomodulating properties of two synthetic adjuvants: Dependence upon type of antigen, dose, and time of administration

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