Coupling phenol bioremediation and biodiesel production by Tetradesmus obliquus: Optimization of phenol removal, biomass productivity and lipid content

Gomaa, Mohamed; El-Naeb, Eman H.; Hifney, Awatief F.; Adam, Mahmoud S.; Fawzy, Mustafa A.

doi:10.1016/j.sajb.2022.10.044

Cited by 4 publications

(3 citation statements)

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“…Furthermore, the simultaneous promotion in both phenol removal efficiency and lipid accumulation at nitrogen-replete conditions suggested that nitrate plays a crucial role in the biodegradation of phenol and its allocation to lipid biosynthesis. The optimum level of nitrates and its ratio to the dissolved organic matter play a crucial role in regulating the growth and biomass production (Ranadheer et al 2019 ; Fawzy et al 2022 ; Gomaa et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, several microalgae have been reported to mineralize phenol completely under sub-lethal concentrations (El-Gendy and Nassar 2021 ). The biodegradation of phenol by microalgae and its utilization as a carbon source for metabolism can increase the biomass productivity with simultaneous accumulation of certain metabolites (Gomaa et al 2022 ). For instance, algal cells tend to accumulate lipids under phenol stress, which may be ascribed as a detoxification mechanism (Das et al 2016 ; Cho et al 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, both autotrophic and heterotrophic mechanisms are present in the mixotrophic mode of growth (Gomaa and Ali 2021 ). The biodegradation of phenol by microalgae was reported to occur under mixotrophic or heterotrophic conditions (Semple and Cain 1996 ; Das et al 2016 ; Gomaa et al 2022 ). Several microalgae have been reported to use phenol as an energy and carbon source such as Chlorella sp., Scenedesmus obliquus , Spirulina sp.…”

Section: Introductionmentioning

confidence: 99%

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Hormesis effects of phenol on growth and cellular metabolites of Chlorella sp. under different nutritional conditions using response surface methodology

Gomaa

El-Naeb

Hifney

et al. 2023

Environ Sci Pollut Res

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The present study investigated the effects of different phenol concentrations (200 – 1000 mg L−1) towards Chlorella sp. under different culture conditions (light vs. dark) and NaNO3 concentrations (0 – 0.1 g L−1) using central composite design. Phenol induced hormesis effects on the algal growth and cellular metabolites. Nitrate was identified as a crucial factor for promoting the uptake of phenol by Chlorella cells, while light was a limiting factor for growth, but the phyco-toxicity of phenol was decreased in the dark. The pigment contents were generally increased in the treated cells to protect against the oxidative phenol stress. The incorporation of 200 mg L−1 phenol and 0.05 g L−1 NaNO3 to the illuminated cells markedly promoted biomass and lipid contents to 0.22 g L−1 and 26.26% w/w, which was 44 and 112% higher than the phenol-less control, respectively. Under the same conditions, the increase of phenol concentration to 600 mg L−1, the protein contents were increased to 18.59% w/w. Conversely, the algal cells were able to accumulate more than 60% w/w of soluble carbohydrates under dark conditions at 600 mg L−1 of phenol. Nitrate replete conditions stimulated lipid accumulation at the expense of protein biosynthesis. Furthermore, most of the treatments showed an increase of H2O2 and malonaldehyde contents, especially for the illuminated cells. However, catalase activity tended to increase under dark conditions, especially at low phenol and nitrate concentrations. This study is valuable in indicating the effects of phenol on microalgae by exploiting response surface methodology, which can be applied as a powerful tool in growth monitoring and toxicity assessment.

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Section: Discussionmentioning

confidence: 99%