Coupling protocol for thin layer chromatography/matrix-assisted laser desorption ionization

Mehl, John T; Gusev, Arkady I.; Hercules, David M.

doi:10.1007/bf02490873

Cited by 54 publications

(26 citation statements)

References 27 publications

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“…Methods for separation of gangliosides by thin layer chromatography (TLC) are well established [14,[43][44][45], and previous work in our own and in other laboratories has shown that TLC can be easily coupled to MALDI-TOF MS [31,46,47], with some compromise in instrument performance due to the irregular surface [31]. The ability to desorb gangliosides without fragmentation, as shown here, allows coupling TLC separations to MALDI-FTMS while retaining spectral quality.…”

Section: Resultsmentioning

confidence: 93%

High pressure matrix-assisted laser desorption/ionization Fourier transform mass spectrometry for minimization of ganglioside fragmentation

O’Connor

Mirgorodskaya

Costello

2002

J. Am. Soc. Mass Spectrom.

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Transiently elevating pressure in a matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS) source into the 1-10 mbar range during ionization decreases the metastable fragmentation of gangliosides. This allows detection of the molecular ion species without loss of the highly labile sialic acid residues. In these experiments, gangliosides with up to five sialic acids were ionized by MALDI and detected with the FTMS. In each case, when the high pressure collisional cooling was used, the singly charged molecular ion was the base peak in the spectra, both in the positive and negative ion modes, and minimal metastable fragmentation was observed. This result is promising, as the previously developed TLC separation methods can be coupled to MALDI-FTMS. (J Am Soc Mass Spectrom 2002, 13, 402-407)

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Section: Resultsmentioning

confidence: 93%

High pressure matrix-assisted laser desorption/ionization Fourier transform mass spectrometry for minimization of ganglioside fragmentation

O’Connor

Mirgorodskaya

Costello

2002

J. Am. Soc. Mass Spectrom.

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“…The dependence of the RSD of the analyte/IS ratio on the number of laser shots was studied by Gusev et al [107,132]. The authors found that the maximum signal intensity was obtained for first 40 laser shots, and the lowest RSD was achieved for the 40 laser shots after the first ten.…”

Section: Quantitative Characterization By Maldimentioning

confidence: 97%

Small molecule analysis by MALDI mass spectrometry

2002

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This review focuses on the application of matrix assisted laser desorption/ionization (MALDI) mass spectrometry to the characterization of molecules in the low mass range (<1500 Da). Despite its reputation to the contrary, MALDI is a powerful technique to provide both qualitative and quantitative determination of low molecular weight compounds. Several approaches to minimize interference via sample preparation and matrix selection are discussed, as well as coupling of MALDI to liquid and planar chromatographic techniques to extend its range of applicability.

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“…Both conditions must be fulfilled without major spreading of the analyte during the addition of the solvents, i.e., without compromising the achieved quality of chromatographic separation. Both important prerequisites were discussed in more detail in (Mehl et al 1997). …”

Section: Tlc/maldi Combinationmentioning

confidence: 98%

TLC/HPTLC with Direct Mass Spectrometric Detection: A Review of the Progress Achieved in the Last 5 Years

Schiller

Fuchs

Teuber

et al. 2010

High-Performance Thin-Layer Chromatography (HPTLC)

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Thin-layer chromatography (TLC) is a widely used, fast and inexpensive method. Even minor constituents of complex mixtures can be often characterized. Unfortunately, the unambiguous assignment of the spots to defined compounds is often difficult because common staining methods normally reveal only (at best) a substance class, not a specific compound. For instance, in the case of phospholipids, the lipid class, but not the detailed fatty acyl composition, can be determined (normal phase TLC). Nowadays mass spectrometry (MS) seems to be the most suitable method for analyte characterization due to its high sensitivity and mass accuracy. This became possible by the invention of soft ionization methods such as matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) MS and electrospray ionization (ESI) although many further desorption techniques are available nowadays. This review summarizes the so far available knowledge about direct TLC/MS couplings and gives an overview about selected compounds that could be successfully analyzed. Chromatographic Methods and Product IdentificationLiquid chromatographic methods, especially high-performance liquid chromatography (HPLC) and planar chromatography [in particular thin-layer (TLC) or highperformance thin-layer chromatography (HPTLC)], are indispensable tools of modern analytical chemistry (Hahn-Deinstrop 2006 l TLC is a very robust system and to a much lesser extent affected by impurities potentially present in the sample. Thus, TLC may also be used for the analysis of "suspicious" samples (for instance, crude tissue or food extracts) that might easily damage or plug the column of an HPLC system. l HPLC columns are normally rather expensive and are, thus, many times reused subsequent to careful washing to remove previous analytes. However, it can never be completely ruled out that there is some residual material on the column. Thus, it is often more difficult to certify an HPLC assay than a TLC assay because TLC does not provide any "memory" effects as a completely new stationary phase is used in all cases. The consumption of solvents is much lower than in the case of HPLC. This makes TLC less expensive and particularly less harmful and more environment friendly.l Although nowadays there are also "multiplexing" HPLC systems available (Woodbury et al. 1995) that allow the simultaneous analysis of several samples in parallel, the simple analysis of a multitude of samples is also a clear advantage of TLC.There is hardly a class of small molecules that have not yet been analyzed by TLC, and thus this review will not deal with improvements of the achievable separation quality, neither by optimizing the stationary phase nor the solvent system nor sample application.However, separation is only one (unequivocally very important) step of successful analysis, and the detection of the different analytes on the TLC plate and their structural elucidation is also very important. This particularly holds because the retardation factor (R f value) by which a com...

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Coupling protocol for thin layer chromatography/matrix-assisted laser desorption ionization

Cited by 54 publications

References 27 publications

High pressure matrix-assisted laser desorption/ionization Fourier transform mass spectrometry for minimization of ganglioside fragmentation

High pressure matrix-assisted laser desorption/ionization Fourier transform mass spectrometry for minimization of ganglioside fragmentation

Small molecule analysis by MALDI mass spectrometry

TLC/HPTLC with Direct Mass Spectrometric Detection: A Review of the Progress Achieved in the Last 5 Years

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