Covalent attachment of cholesterol oxidase and horseradish peroxidase on perlite through silanization: Activity, stability and co-immobilization

Torabi, Seyed-Fakhreddin; Khajeh, Khosro; Ghasempur, Salehe; Ghaemi, Nasser; Siadat, Seyed-Omid Ranaei

doi:10.1016/j.jbiotec.2007.04.015

Cited by 72 publications

(48 citation statements)

References 46 publications

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“…The optimum pH values of the enzyme activity were found as 8 and 7 for the free HRP and the immobilized HRP respectively [21]. Based on Torabi et al [22] covalently immobilized HRP on perlite surface activated with 3-aminopropytriethoxysilane via glutaraldehyde can cause change optimum pH of enzymes to the lower pH exactly. On the other hand, Fernandes et al [23] also reported that pH range of HRP increased after immobilization.…”

Section: Optimum Phmentioning

confidence: 97%

Immobilization Of Horseradish Peroxidase On Nanopolyaniline Surface Using Self Assembly Techniques For Biosensor Applications

Amira¹,

Ain²

2016

Fifth International Conference on Advances in Applied Science and Environmental Engineering - ASEE 2016

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Abstract-This review paper describes the immobilization of horseradish peroxidase (HRP) on nanopolyaniline (PANI) by using self-assembly technique. Some chemically synthesized conductive polymers such as PANI were used as support for HRP immobilization. Enzymes immobilized were depend on their properties of immobilize of HRP such as optimum pH, thermal stability and storage stability and all these were investigated. The structure of PANI was characterized by FTIR, FESEM and UV-VIS. The structure of PANI depends on the pH of the phosphate buffer and the type of organic solvent. Thus, the formation of monolayer especially nano-particles of PANI was prepared by using Langmuir-Blodgett technique. The contribution in the development of the biosensor application for hydrogen peroxidase (H 2 O 2 ) based on the HRP modified electrode was described.

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Section: Optimum Phmentioning

confidence: 97%

Immobilization Of Horseradish Peroxidase On Nanopolyaniline Surface Using Self Assembly Techniques For Biosensor Applications

Amira¹,

Ain²

2016

Fifth International Conference on Advances in Applied Science and Environmental Engineering - ASEE 2016

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“…The pH working range was shifted downward by one pH unit from 8.0-9.0 to 7.0-9.0 while during the removal of p-chlorophenol, pH range was also broadened toward acidic range with one pH unit from 7.5-9.5 to 6.5-9.5. Torabi et al [18] covalently immobilized HRP on perlite surface activated with 3-aminopropyltriethoxysilane via glutaraldehyde. Immobilization shifted optimum pH of enzymes to the lower pH.…”

Section: Ph Working Rangementioning

confidence: 99%

Cross-linking of Horseradish Peroxidase Enzyme to Fine Particles Generated by Nano Spray Dryer B-90

Dahili¹,

Feczkó²

2015

Period. Polytech. Chem. Eng.

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“…The studies conducted in the current decade, show that the effective Fenton-type catalysts are Fecontaining materials obtained by fixation of iron oxides on aluminosilicate matrix of clay minerals [14]. These materials have a large specific surface area and high iron content, and are of great interest as Fenton and photo-Fenton catalysts for processes of wastewater treatment.…”

Section: Introductionmentioning

confidence: 99%

Catalytic Properties of Fe-containing Layered Aluminosilicates in Photo-oxidation of Dye “Methyl Green”

Shadrina

Dashinamzhilova

Khankhasaeva

2017

IOP Conf. Ser.: Mater. Sci. Eng.

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Abstract. The iron-containing materials with an iron content of 40 mg/g and 52.5 mg/g, a specific surface area of 107 m 2 /g and 96 m 2 /g are developed on the basis of natural layered aluminosilicate (montmorillonite) and polyhydroxo complexes of iron. It is shown that the materials exhibit high catalytic activity in the photo-oxidation of dye "Methyl Green". The influence of physicochemical parameters (loading of the catalyst, a ratio of initial concentrations [H 2 O 2 ]/[MG] on the efficiency of the dye photo-oxidation was established. The optimum conditions, which made it possible to achieve high mineralization and 100 % the dye oxidation efficiency were determined: the catalyst loading equal to 1.0 g/l and the ratio of [H 2 O 2 ] and [MG] equal to stoichiometric ratio (55 mol/mol). The decrease of the total organic carbon content after photo-oxidation reaction was 56.5%. The average value of the quantum yield of the dye photo-oxidation was to 0.30 mol/Einstein. The results of the conducted research show that the developed iron-containing materials are the promising catalysts for photo-Fenton processes of oxidative degradation of organic compounds. The materials are of interest for use in wastewater treatment processes from toxic organic pollutants.

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Covalent attachment of cholesterol oxidase and horseradish peroxidase on perlite through silanization: Activity, stability and co-immobilization

Cited by 72 publications

References 46 publications

Immobilization Of Horseradish Peroxidase On Nanopolyaniline Surface Using Self Assembly Techniques For Biosensor Applications

Immobilization Of Horseradish Peroxidase On Nanopolyaniline Surface Using Self Assembly Techniques For Biosensor Applications

Cross-linking of Horseradish Peroxidase Enzyme to Fine Particles Generated by Nano Spray Dryer B-90

Catalytic Properties of Fe-containing Layered Aluminosilicates in Photo-oxidation of Dye “Methyl Green”

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